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ArXiv
ArXiv
arxiv
ArXiv
2331-8422
Cornell University

arXiv:2310.13803v4
2310.13803
4
preprint
Article
Noise robustness and metabolic load determine the principles of central dogma regulation
Lo Teresa W.
Choi Han James
Huang Dean
Wiggins Paul A.
15 8 2024
arXiv:2310.13803v420 10 2023
https://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License, which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.
http://arxiv.org/abs/2310.13803v4
nihpp-2310.13803v4.pdf
The processes of gene expression are inherently stochastic, even for essential genes required for growth. How does the cell maximize fitness in light of noise? To answer this question, we build a mathematical model to explore the trade-off between metabolic load and growth robustness. The model predicts novel principles of central dogma regulation: Optimal protein expression levels for many genes are in vast overabundance. Essential genes are transcribed above a lower limit of one message per cell cycle. Gene expression is achieved by load balancing between transcription and translation. We present evidence that each of these novel regulatory principles is observed. These results reveal that robustness and metabolic load determine the global regulatory principles that govern gene expression processes, and these principles have broad implications for cellular function.

Main: 10 pages, 6 figures; Supplemental Material: 27 pages, 12 figures
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