
==== Front
Wellcome Open Res
Wellcome Open Res
Wellcome Open Research
2398-502X
F1000 Research Limited London, UK

10.12688/wellcomeopenres.21665.1
Data Note
Articles
The genome sequence of the Blue-bordered Carpet moth Plemyria rubiginata (Denis & Schiffermüller) 1775
[version 1; peer review: 2 approved]

Boyes Douglas Investigation Resources https://orcid.org/0000-0002-8283-6324
1
Lees David C. Investigation Resources Writing – Review & Editing https://orcid.org/0000-0002-7580-8560
2
Wawman Denise C. Writing – Original Draft Preparation https://orcid.org/0000-0002-8172-4734
3
University of Oxford and Wytham Woods Genome Acquisition Lab
Natural History Museum Genome Acquisition Lab
Darwin Tree of Life Barcoding collective
Wellcome Sanger Institute Tree of Life Management, Samples and Laboratory team
Wellcome Sanger Institute Scientific Operations: Sequencing Operations
Wellcome Sanger Institute Tree of Life Core Informatics team
Tree of Life Core Informatics collective
Darwin Tree of Life Consortiuma
1 UK Centre for Ecology & Hydrology, Wallingford, England, UK
2 Natural History Museum, London, England, UK
3 Edward Grey Institute, Department of Biology, University of Oxford, Oxford, England, UK
a mark.blaxter@sanger.ac.uk
+ Deceased author

No competing interests were disclosed.

16 5 2024
2024
9 27110 5 2024
Copyright: © 2024 Boyes D et al.
2024
https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

We present a genome assembly from an individual female Plemyria rubiginata (the Blue-bordered Carpet moth; Arthropoda; Insecta; Lepidoptera; Geometridae). The genome sequence is 356.2 megabases in span. Most of the assembly is scaffolded into 30 chromosomal pseudomolecules, including the Z and W sex chromosomes. The mitochondrial genome has also been assembled and is 17.64 kilobases in length.

Plemyria rubiginata
Blue-bordered Carpet moth
genome sequence
chromosomal
Lepidoptera
Wellcome Trust206194 218328 This work was supported by Wellcome through core funding to the Wellcome Sanger Institute [206194, <a href=https://doi.org/10.35802/206194>https://doi.org/10.35802/206194</a>] and the Darwin Tree of Life Discretionary Award [218328, <a href=https://doi.org/10.35802/218328>https://doi.org/10.35802/218328 </a>]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
==== Body
pmcSpecies taxonomy

Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia; Obtectomera; Geometroidea; Geometridae; Larentiinae; Plemyria; Plemyria rubiginata (Denis & Schiffermüller) 1775 (NCBI:txid934957).

Background

The Blue-bordered Carpet Plemyria rubiginata ([Denis & Schiffermüller], 1775) is a moth in the family Geometridae with a narrow forewing 12–15 mm long (typically 21–26 mm wingspan; Hausmann & Viidalepp, 2012).

Two subspecies have traditionally been recognised in the United Kingdom: P. rubiginata rubiginata with a whitish background and blue border, and a large brown blotch on the leading edge of the wing; and 'subspecies’ P. rubiginata plumbata (Curtis, 1837), distinguished by the brown blotch extending to become a full crossband, a darker and brownish border, and a ground colour of greyish-beige ( Waring et al., 2017). However, the latter is internationally treated as f. plumbata (possibly an example of increasing northwards melanism at higher latitudes and elevations in northern England and Scotland, also in Norway and possibly Japan). By contrast, P. rubiginata japonica Inoue, 1955 is treated as validly distinct from the nominotypical subspecies from eastern Siberia and Mongolia to Japan ( Hausmann & Viidalepp, 2012). Randle et al. (2019) show a continuous distribution, although P. rubiginata is more scattered in Scotland and more localised in Ireland. Broadly, the species spans from 0–1600 m across almost the entire Palaearctic from north-western Portugal and Spain to China and Japan and the southern shores of the Mediterranean to northern Scandinavia and north-western Russia, with an isolated population in Turkey ( GBIF Secretariat, 2024; BOLD accessed 08/04/2024; Hausmann & Viidalepp, 2012).

Despite not being a species observed in large numbers, the Blue-bordered Carpet has shown a dramatic increase in abundance of 173% since 1970, although no change in distribution ( Randle et al., 2019). The moth is univoltine, flying between early June and late September in the UK with some sign of a shift to an earlier modal abundance ( Randle et al., 2019); and flying from mid-July at higher elevations in Europe ( Hausmann & Viidalepp, 2012).

P. rubiginata overwinters as an egg on the twigs of its foodplant, and the greenish larvae with yellow lateral stripes feed among spinnings on twig from April to June, preferring alder Alnus glutinosa (L.) Gaertn. or blackthorn Prunus spinosa L., but are also found on apple Malus spp., plum Prunus spp., hawthorn Crataegus monogyna Jacq. and birch Betula spp. ( Sterling et al., 2012; Waring et al., 2017) foodplants also utilised by the Nearctic sister species, P. georgii Hulst, 1896. ( Choi, 1998),

P. rubiginata occupies a single BIN cluster on BOLD (08/04/2024), BOLD:AAC1712, with up to 2.77% pairwise divergence within it ( n = 33), with Russian and Chinese exemplars showing highest divergences (~1.43% to the nearest exemplars). There are eight exemplars DNA barcoded from the UK (08/04/2024), the most northerly being from Lancashire but none so far from Scotland and Ireland, but at present there seems to be no mitochondrial evidence for genetic distinctiveness of northern UK populations. The Nearctic P. georgii (BOLD:AAB0097) by contrast is at least 5.65% divergent from it (although a species of Chloroclystis, BOLD:AFD5638, approaches P. rubiginata more closely at 4.17%).

The morphology and taxonomy of Plemyria Hübner, 1825 was reviewed by Choi (1998) and Hausmann & Viidalepp (2012), the genus comprising just the above two species. Plemyria belongs to the larentiinae tribe Cidariini. Based on a morphological analysis of adults and immatures, Choi (1997) placed the genus adjacent to Chloroclystis Hübner, 1825 and sister to Thera Stephens, 1831 + ( Pennithera Viidalepp, 1980 + Heterothera Inoue, 1943). The genus was not, however, included in the molecular larentiine phylogeny of Õunap et al. (2016).

The genome will be useful in investigating industrial melanism, stimulating more DNA barcoding, and clarifying the phylogeography of the species and the history of its sister species split, as well as establishing the phylogenetic placement of Plemyria.

Genome sequence report

The genome was sequenced from one female Plemyria rubiginata ( Figure 1) collected from Wytham Woods, Oxfordshire, UK (51.76, –1.34). A total of 94-fold coverage in Pacific Biosciences single-molecule HiFi long reads was generated. Primary assembly contigs were scaffolded with chromosome conformation Hi-C data. Manual assembly curation corrected 15 missing joins or mis-joins and removed 8 haplotypic duplications, reducing the assembly length by 1.07% and the scaffold number by 9.80%.

Figure 1. Photograph of the Plemyria rubiginata (ilPleRubi1) specimen used for genome sequencing.

The final assembly has a total length of 356.2 Mb in 45 sequence scaffolds with a scaffold N50 of 12.8 Mb ( Table 1). The snail plot in Figure 2 provides a summary of the assembly statistics, while the distribution of assembly scaffolds on GC proportion and coverage is shown in Figure 3. The cumulative assembly plot in Figure 4 shows curves for subsets of scaffolds assigned to different phyla. Most (99.87%) of the assembly sequence was assigned to 30 chromosomal-level scaffolds, representing 28 autosomes and the Z and W sex chromosomes. Chromosome-scale scaffolds confirmed by the Hi-C data are named in order of size ( Figure 5; Table 2). While not fully phased, the assembly deposited is of one haplotype. Contigs corresponding to the second haplotype have also been deposited. The mitochondrial genome was also assembled and can be found as a contig within the multifasta file of the genome submission.

Table 1. Genome data for Plemyria rubiginata, ilPleRubi1.1.

Project accession data	
Assembly identifier	ilPleRubi1.1	
Species	Plemyria rubiginata	
Specimen	ilPleRubi1	
NCBI taxonomy ID	934957	
BioProject	PRJEB65207	
BioSample ID	SAMEA7701302	
Isolate information	ilPleRubi1, female: whole organism (DNA sequencing)
ilPleRubi2: whole organism (Hi-C and RNA sequencing)	
Assembly metrics *	Benchmark	
Consensus quality (QV)	63.1	≥ 50	
k-mer completeness	100.0%	≥ 95%	
BUSCO **	C:98.5%[S:97.9%,D:0.6%],F:0.3%,M:1.2%,n:5,286	C ≥ 95%	
Percentage of assembly mapped to chromosomes	99.87%	≥ 95%	
Sex chromosomes	ZW	localised homologous pairs	
Organelles	Mitochondrial genome: 17.64 kb	complete single alleles	
Raw data accessions	
PacificBiosciences Sequel IIe	ERR11867205, ERR11867206	
Hi-C Illumina	ERR11872574	
PolyA RNA-Seq Illumina	ERR12245586	
Genome assembly	
Assembly accession	GCA_963576535.1	
Accession of alternate haplotype	GCA_963576495.1	
Span (Mb)	356.2	
Number of contigs	68	
Contig N50 length (Mb)	11.8	
Number of scaffolds	45	
Scaffold N50 length (Mb)	12.8	
Longest scaffold (Mb)	15.64	
* Assembly metric benchmarks are adapted from column VGP-2020 of “Table 1: Proposed standards and metrics for defining genome assembly quality” from Rhie et al. (2021).

** BUSCO scores based on the lepidoptera_odb10 BUSCO set using version v5.4.3. C = complete [S = single copy, D = duplicated], F = fragmented, M = missing, n = number of orthologues in comparison. A full set of BUSCO scores is available at https://blobtoolkit.genomehubs.org/view/Plemyria_rubiginata/dataset/GCA_963576535.1/busco.

Figure 2. Genome assembly of Plemyria rubiginata, ilPleRubi1.1: metrics.

The BlobToolKit snail plot shows N50 metrics and BUSCO gene completeness. The main plot is divided into 1,000 size-ordered bins around the circumference with each bin representing 0.1% of the 356,234,577 bp assembly. The distribution of scaffold lengths is shown in dark grey with the plot radius scaled to the longest scaffold present in the assembly (15,641,424 bp, shown in red). Orange and pale-orange arcs show the N50 and N90 scaffold lengths (12,846,343 and 8,926,186 bp), respectively. The pale grey spiral shows the cumulative scaffold count on a log scale with white scale lines showing successive orders of magnitude. The blue and pale-blue area around the outside of the plot shows the distribution of GC, AT and N percentages in the same bins as the inner plot. A summary of complete, fragmented, duplicated and missing BUSCO genes in the lepidoptera_odb10 set is shown in the top right. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/Plemyria_rubiginata/dataset/GCA_963576535.1/snail.

Figure 3. Genome assembly of Plemyria rubiginata, ilPleRubi1.1: BlobToolKit GC-coverage plot.

Sequences are coloured by phylum. Circles are sized in proportion to sequence length. Histograms show the distribution of sequence length sum along each axis. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/Plemyria_rubiginata/dataset/GCA_963576535.1/blob.

Figure 4. Genome assembly of Plemyria rubiginata, ilPleRubi1.1: BlobToolKit cumulative sequence plot.

The grey line shows cumulative length for all sequences. Coloured lines show cumulative lengths of sequences assigned to each phylum using the buscogenes taxrule. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/Plemyria_rubiginata/dataset/GCA_963576535.1/cumulative.

Figure 5. Genome assembly of Plemyria rubiginata, ilPleRubi1.1: Hi-C contact map of the ilPleRubi1.1 assembly, visualised using HiGlass.

Chromosomes are shown in order of size from left to right and top to bottom. An interactive version of this figure may be viewed at https://genome-note-higlass.tol.sanger.ac.uk/l/?d=N6e0u10NSg-QOuCMZCLXhQ.

Table 2. Chromosomal pseudomolecules in the genome assembly of Plemyria rubiginata, ilPleRubi1.

INSDC accession	Chromosome	Length (Mb)	GC%	
OY754906.1	1	15.54	38.0	
OY754907.1	2	15.54	38.0	
OY754908.1	3	14.99	38.0	
OY754909.1	4	14.82	38.0	
OY754910.1	5	13.99	37.5	
OY754911.1	6	13.97	38.0	
OY754912.1	7	13.87	37.5	
OY754913.1	8	13.47	38.0	
OY754914.1	9	13.31	37.5	
OY754915.1	10	13.13	37.5	
OY754916.1	11	13.09	37.5	
OY754917.1	12	12.85	38.0	
OY754918.1	13	12.76	38.0	
OY754919.1	14	12.3	37.5	
OY754920.1	15	12.3	37.5	
OY754921.1	16	12.25	38.0	
OY754922.1	17	12.12	38.5	
OY754923.1	18	11.96	38.0	
OY754924.1	19	11.83	38.0	
OY754925.1	20	11.73	38.0	
OY754926.1	21	11.09	38.0	
OY754927.1	22	10.6	38.5	
OY754928.1	23	10.65	38.0	
OY754929.1	24	8.93	39.0	
OY754930.1	25	8.44	38.0	
OY754931.1	26	7.39	38.0	
OY754932.1	27	6.13	39.5	
OY754933.1	28	5.64	38.5	
OY754934.1	W	2.41	40.0	
OY754905.1	Z	15.64	38.0	
OY754935.1	MT	0.02	18.5	

The estimated Quality Value (QV) of the final assembly is 63.1 with k-mer completeness of 100.0%, and the assembly has a BUSCO v completeness of 98.5% (single = 97.9%, duplicated = 0.6%), using the lepidoptera_odb10 reference set ( n = 5,286).

Metadata for specimens, BOLD barcode results, spectra estimates, sequencing runs, contaminants and pre-curation assembly statistics are given at https://links.tol.sanger.ac.uk/species/934957.

Methods

Sample acquisition and nucleic acid extraction

A female Plemyria rubiginata (specimen ID Ox000535, ToLID ilPleRubi1) was collected from Wytham Woods, Oxfordshire (biological vice-county Berkshire), UK (latitude 51.76, longitude –1.34) on 2020-06-25 using a light trap. The specimen was collected and identified by Douglas Boyes (University of Oxford) and preserved on dry ice.

The specimen used for Hi-C and RNA sequencing (specimen ID NHMUK014584832, ToLID ilPleRubi2) was hand-picked in Lucas Wood, High Wycombe, London (latitude 51.63, longitude –0.74) on 2022-06-23. The specimen was collected and identified by David Lees (Natural History Museum) and preserved by dry-freezing at –80 °C.

The workflow for high molecular weight (HMW) DNA extraction at the Wellcome Sanger Institute (WSI) Tree of Life Core Laboratory includes a sequence of core procedures: sample preparation; sample homogenisation, DNA extraction, fragmentation, and clean-up. In sample preparation, the ilPleRubi1 sample was weighed and dissected on dry ice ( Jay et al., 2023). Tissue from the whole organism was homogenised using a PowerMasher II tissue disruptor ( Denton et al., 2023a).

HMW DNA was extracted using the Automated MagAttract v1 protocol ( Sheerin et al., 2023). DNA was sheared into an average fragment size of 12–20 kb in a Megaruptor 3 system with speed setting 30 ( Todorovic et al., 2023). Sheared DNA was purified by solid-phase reversible immobilisation ( Strickland et al., 2023): in brief, the method employs a 1.8X ratio of AMPure PB beads to sample to eliminate shorter fragments and concentrate the DNA. The concentration of the sheared and purified DNA was assessed using a Nanodrop spectrophotometer and Qubit Fluorometer and Qubit dsDNA High Sensitivity Assay kit. Fragment size distribution was evaluated by running the sample on the FemtoPulse system.

RNA was extracted from tissue of ilPleRubi2 in the Tree of Life Laboratory at the WSI using the RNA Extraction: Automated MagMax™ mirVana protocol ( do Amaral et al., 2023). The RNA concentration was assessed using a Nanodrop spectrophotometer and a Qubit Fluorometer using the Qubit RNA Broad-Range Assay kit. Analysis of the integrity of the RNA was done using the Agilent RNA 6000 Pico Kit and Eukaryotic Total RNA assay.

Protocols developed by the WSI Tree of Life laboratory are publicly available on protocols.io ( Denton et al., 2023b).

Sequencing

Pacific Biosciences HiFi circular consensus DNA sequencing libraries were constructed according to the manufacturers’ instructions. Poly(A) RNA-Seq libraries were constructed using the NEB Ultra II RNA Library Prep kit. DNA and RNA sequencing was performed by the Scientific Operations core at the WSI on Pacific Biosciences Sequel IIe (HiFi) and Illumina NovaSeq 6000 (RNA-Seq) instruments. Hi-C data were also generated from tissue of ilPleRubi2 using the Arima2 kit and sequenced on the Illumina NovaSeq 6000 instrument.

Genome assembly and curation

Assembly was carried out with Hifiasm ( Cheng et al., 2021) and haplotypic duplication was identified and removed with purge_dups ( Guan et al., 2020). The assembly was then scaffolded with Hi-C data ( Rao et al., 2014) using YaHS ( Zhou et al., 2023). The assembly was checked for contamination and corrected using the TreeVal pipeline ( Pointon et al., 2023). Manual curation was performed using JBrowse2 ( Diesh et al., 2023), HiGlass ( Kerpedjiev et al., 2018) and PretextView ( Harry, 2022). The mitochondrial genome was assembled using MitoHiFi ( Uliano-Silva et al., 2023), which runs MitoFinder ( Allio et al., 2020) or MITOS ( Bernt et al., 2013) and uses these annotations to select the final mitochondrial contig and to ensure the general quality of the sequence.

Final assembly evaluation

The final assembly was post-processed and evaluated with the three Nextflow ( Di Tommaso et al., 2017) DSL2 pipelines “sanger-tol/readmapping” ( Surana et al., 2023a), “sanger-tol/genomenote” ( Surana et al., 2023b), and “sanger-tol/blobtoolkit” ( Muffato et al., 2024). The pipeline sanger-tol/readmapping aligns the Hi-C reads with bwa-mem2 ( Vasimuddin et al., 2019) and combines the alignment files with SAMtools ( Danecek et al., 2021). The sanger-tol/genomenote pipeline transforms the Hi-C alignments into a contact map with BEDTools ( Quinlan & Hall, 2010) and the Cooler tool suite ( Abdennur & Mirny, 2020), which is then visualised with HiGlass ( Kerpedjiev et al., 2018). It also provides statistics about the assembly with the NCBI datasets ( Sayers et al., 2024) report, computes k-mer completeness and QV consensus quality values with FastK and MerquryFK, and a completeness assessment with BUSCO ( Manni et al., 2021).

The sanger-tol/blobtoolkit pipeline is a Nextflow port of the previous Snakemake Blobtoolkit pipeline ( Challis et al., 2020). It aligns the PacBio reads with SAMtools and minimap2 ( Li, 2018) and generates coverage tracks for regions of fixed size. In parallel, it queries the GoaT database ( Challis et al., 2023) to identify all matching BUSCO lineages to run BUSCO ( Manni et al., 2021). For the three domain-level BUSCO lineage, the pipeline aligns the BUSCO genes to the Uniprot Reference Proteomes database ( Bateman et al., 2023) with DIAMOND ( Buchfink et al., 2021) blastp. The genome is also split into chunks according to the density of the BUSCO genes from the closest taxonomically lineage, and each chunk is aligned to the Uniprot Reference Proteomes database with DIAMOND blastx. Genome sequences that have no hit are then chunked with seqtk and aligned to the NT database with blastn ( Altschul et al., 1990). All those outputs are combined with the blobtools suite into a blobdir for visualisation.

All three pipelines were developed using the nf-core tooling ( Ewels et al., 2020), use MultiQC ( Ewels et al., 2016), and make extensive use of the Conda package manager, the Bioconda initiative ( Grüning et al., 2018), the Biocontainers infrastructure ( da Veiga Leprevost et al., 2017), and the Docker ( Merkel, 2014) and Singularity ( Kurtzer et al., 2017) containerisation solutions.

Table 3 contains a list of relevant software tool versions and sources.

Table 3. Software tools: versions and sources.

Software tool	Version	Source	
BEDTools	2.30.0	https://github.com/arq5x/bedtools2	
Blast	2.14.0	ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/	
BlobToolKit	4.3.7	https://github.com/blobtoolkit/blobtoolkit	
BUSCO	5.4.3 and 5.5.0	https://gitlab.com/ezlab/busco	
bwa-mem2	2.2.1	https://github.com/bwa-mem2/bwa-mem2	
Cooler	0.8.11	https://github.com/open2c/cooler	
DIAMOND	2.1.8	https://github.com/bbuchfink/diamond	
fasta_windows	0.2.4	https://github.com/tolkit/fasta_windows	
FastK	427104ea91c78c3b8b8b49f1a7d6bbeaa869ba1c	https://github.com/thegenemyers/FASTK	
GoaT CLI	0.2.5	https://github.com/genomehubs/goat-cli	
Hifiasm	0.19.5-r587	https://github.com/chhylp123/hifiasm	
HiGlass	44086069ee7d4d3f6f3f0012569789ec138f42b84aa44357826c0b6753eb28de	https://github.com/higlass/higlass	
MerquryFK	d00d98157618f4e8d1a9190026b19b471055b22e	https://github.com/thegenemyers/MERQURY.FK	
MitoHiFi	3	https://github.com/marcelauliano/MitoHiFi	
MultiQC	1.14, 1.17, and 1.18	https://github.com/MultiQC/MultiQC	
NCBI Datasets	15.12.0	https://github.com/ncbi/datasets	
Nextflow	23.04.0-5857	https://github.com/nextflow-io/nextflow	
PretextView	0.2	https://github.com/wtsi-hpag/PretextView	
purge_dups	1.2.5	https://github.com/dfguan/purge_dups	
samtools	1.16.1, 1.17, and 1.18	https://github.com/samtools/samtools	
sanger-tol/genomenote	1.1.1	https://github.com/sanger-tol/genomenote	
sanger-tol/readmapping	1.2.1	https://github.com/sanger-tol/readmapping	
Seqtk	1.3	https://github.com/lh3/seqtk	
Singularity	3.9.0	https://github.com/sylabs/singularity	
TreeVal	1.0.0	https://github.com/sanger-tol/treeval	
YaHS	1.2a.2	https://github.com/c-zhou/yahs	

Wellcome Sanger Institute – Legal and Governance

The materials that have contributed to this genome note have been supplied by a Darwin Tree of Life Partner. The submission of materials by a Darwin Tree of Life Partner is subject to the ‘Darwin Tree of Life Project Sampling Code of Practice’, which can be found in full on the Darwin Tree of Life website here. By agreeing with and signing up to the Sampling Code of Practice, the Darwin Tree of Life Partner agrees they will meet the legal and ethical requirements and standards set out within this document in respect of all samples acquired for, and supplied to, the Darwin Tree of Life Project.

Further, the Wellcome Sanger Institute employs a process whereby due diligence is carried out proportionate to the nature of the materials themselves, and the circumstances under which they have been/are to be collected and provided for use. The purpose of this is to address and mitigate any potential legal and/or ethical implications of receipt and use of the materials as part of the research project, and to ensure that in doing so we align with best practice wherever possible. The overarching areas of consideration are:

•   Ethical review of provenance and sourcing of the material

•   Legality of collection, transfer and use (national and international)

Each transfer of samples is further undertaken according to a Research Collaboration Agreement or Material Transfer Agreement entered into by the Darwin Tree of Life Partner, Genome Research Limited (operating as the Wellcome Sanger Institute), and in some circumstances other Darwin Tree of Life collaborators.

Data availability

European Nucleotide Archive: Plemyria rubiginata (blue-bordered carpet). Accession number PRJEB65207; https://identifiers.org/ena.embl/PRJEB65207 ( Wellcome Sanger Institute, 2023). The genome sequence is released openly for reuse. The Plemyria rubiginata genome sequencing initiative is part of the Darwin Tree of Life (DToL) project. All raw sequence data and the assembly have been deposited in INSDC databases. The genome will be annotated using available RNA-Seq data and presented through the Ensembl pipeline at the European Bioinformatics Institute. Raw data and assembly accession identifiers are reported in Table 1.

Author information

Members of the University of Oxford and Wytham Woods Genome Acquisition Lab are listed here: https://doi.org/10.5281/zenodo.7125292.

Members of the Natural History Museum Genome Acquisition Lab are listed here: https://doi.org/10.5281/zenodo.7139035.

Members of the Darwin Tree of Life Barcoding collective are listed here: https://doi.org/10.5281/zenodo.4893703.

Members of the Wellcome Sanger Institute Tree of Life Management, Samples and Laboratory team are listed here: https://doi.org/10.5281/zenodo.10066175.

Members of Wellcome Sanger Institute Scientific Operations: Sequencing Operations are listed here: https://doi.org/10.5281/zenodo.10043364.

Members of the Wellcome Sanger Institute Tree of Life Core Informatics team are listed here: https://doi.org/10.5281/zenodo.10066637.

Members of the Tree of Life Core Informatics collective are listed here: https://doi.org/10.5281/zenodo.5013541.

Members of the Darwin Tree of Life Consortium are listed here: https://doi.org/10.5281/zenodo.4783558.

10.21956/wellcomeopenres.23938.r99779
Reviewer response for version 1
Arya Varun 1Referee https://orcid.org/0000-0002-0901-5693

1 Entomology & Agricultural Zoology, Banaras Hindu University (Ringgold ID: 30114), Varanasi, Uttar Pradesh, India
20 9 2024 Copyright: © 2024 Arya V
2024
https://creativecommons.org/licenses/by/4.0/ This is an open access peer review report distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Version 1recommendationapprove
This study presents a comprehensive genomic assembly of Plemyria rubiginata, commonly referred to as the 'blue-bordered carpet moth.' As present in the manuscript, the assembled sequences were successfully mapped to both autosomes and sex chromosomes.

Minor comments: Please make the following changes in the keywords: italicize “ Plemyria rubiginata.” Keep “Blue-bordered Carpet moth” in lower case, also in other places in the manuscript while writing. The name of the insect orders, if used in the middle of the sentence, shall not be capitalized. Write “Lepidoptera” in lower case.

In the background: instead of “([Denis & Schiffermüller], 1775)”, write it as “(Denis & Schiffermüller, 1775)”. Also correct it in the title. In the manuscript, “However, the latter is internationally treated as f. plumbata”, the identity of “ f. plumbata” is not clear if the authors have mentioned it as a distinct sub species or is it a typing error. The citation of “([Denis & Schiffermüller], 1775)” can be rewritten as “(Denis & Schiffermüller, 1775)” in both the background and the title.

Are sufficient details of methods and materials provided to allow replication by others?

Yes

Is the rationale for creating the dataset(s) clearly described?

Yes

Are the datasets clearly presented in a useable and accessible format?

Yes

Are the protocols appropriate and is the work technically sound?

Yes

Reviewer Expertise:

Entomology & Agricultural Zoology, Plant Protection, Molecular Entomology, Insect Ecology

I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard.

10.21956/wellcomeopenres.23938.r97168
Reviewer response for version 1
Havill Nathan P 1Referee
1 Northern Research Station, Hamden, USA
10 9 2024 Copyright: © 2024 Havill NP
2024
https://creativecommons.org/licenses/by/4.0/ This is an open access peer review report distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Version 1recommendationapprove
The article, "The genome sequence of the Blue-bordered Carpet moth  Plemyria rubiginata..." reports a genome high quality assembly for a female individual of this species. The methods and analyses appear to be robust, and the genome sequence is a welcome addition to the growing number of lepidopteran examples. I have only minor edits to suggest below:

Title: Do not capitalize “Blue” and “Carpet”. Also, change to, “… Plemyria rubiginata (Denis & Schiffermüller, 1775).

Keywords: Please replace the words that are already in the title with others.

Background: Change to, “… Plemyria rubiginata (Denis & Schiffermüller, 1775).

Instead of “.. treated as f. plumbata,” do you mean “treated as P. plumbata” ?

This might be better: “By contrast, P . rubiginata japonica Inoue, 1955, from eastern Siberia and Mongolia to Japan, is treated as validly distinct from the nominotypical subspecies (Hausmann & Viidalepp, 2012).

From the sentence construction, is not clear which food plants are also utilized by P. georgii. All of them? Please make this more clear.

This might be better: “The morphology and taxonomy of Plemyria Hübner, 1825, the genus comprising just the above two species, was reviewed by Choi (1998) and Hausmann & Viidalepp (2012).”

Are sufficient details of methods and materials provided to allow replication by others?

Yes

Is the rationale for creating the dataset(s) clearly described?

Yes

Are the datasets clearly presented in a useable and accessible format?

Yes

Are the protocols appropriate and is the work technically sound?

Yes

Reviewer Expertise:

Insect population genetics and phylogenetics.

I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard.

Competing interests: No competing interests were disclosed.

Competing interests: No competing interests were disclosed.
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