
==== Front
Int J Hematol
Int J Hematol
International Journal of Hematology
0925-5710
1865-3774
Springer Nature Singapore Singapore

39136895
3830
10.1007/s12185-024-03830-z
Original Article
A lower initial dose of bosutinib for patients with chronic myeloid leukemia patients resistant and/or intolerant to prior therapy: a single-arm, multicenter, phase 2 trial (BOGI trial)
Ureshino Hiroshi 1
http://orcid.org/0000-0002-6758-3787
Takahashi Naoto naotot@doc.med.akita-u.ac.jp

2
Ikezoe Takayuki 3
Kameoka Yoshihiro 2
Kimura Satoshi 3
Fukushima Noriyasu 4
Ichinohe Tatsuo 1
Takamori Ayako 5
Kawaguchi Atsushi 6
Miura Masatomo 7
Kimura Shinya 8
1 https://ror.org/03t78wx29 grid.257022.0 0000 0000 8711 3200 Department of Hematology and Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan
2 https://ror.org/03hv1ad10 grid.251924.9 0000 0001 0725 8504 Department of Hematology, Nephrology and Rheumatology, Akita University Graduate School of Medicine, 1‑1‑1, Hondo, Akita, Akita 010‑8543 Japan
3 https://ror.org/012eh0r35 grid.411582.b 0000 0001 1017 9540 Department of Hematology, Fukushima Medical University, Fukushima, Japan
4 Department of Internal Medicine, Karatsu Red Cross Hospital, Karatsu, Japan
5 https://ror.org/04f4wg107 grid.412339.e 0000 0001 1172 4459 Clinical Research Center, Saga University Hospital, Saga, Japan
6 https://ror.org/04f4wg107 grid.412339.e 0000 0001 1172 4459 Education and Research Center for Community Medicine, Faculty of Medicine, Saga University, Saga, Japan
7 https://ror.org/03hv1ad10 grid.251924.9 0000 0001 0725 8504 Department of Pharmacokinetics, Akita University Graduate School of Medicine, Akita, Japan
8 https://ror.org/04f4wg107 grid.412339.e 0000 0001 1172 4459 Hematology, Respiratory Medicine and Oncology, Department of Internal Medicine, Saga University, Saga, Japan
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© The Author(s) 2024
2024
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Although bosutinib is generally safe and effective, drug-related toxicities (DRTs) such as diarrhea or increased transaminase levels often lead to treatment discontinuation. To clarify whether a lower initial dose of bosutinib (i.e., starting at 200 mg) would reduce rates of discontinuation due to DRTs, we conducted a phase 2 study of BOsutinib Gradual Increase (BOGI trial, UMIN 000032282) as a second/third-line treatment for chronic myeloid leukemia (CML). Between February 4, 2019 and May 24, 2022, 35 patients were enrolled. The rate of bosutinib discontinuation at 12 months was 25.7% vs. 35.9% in a historical control study (Japanese phase 1/2 study) (p = 0.102). The rate of bosutinib discontinuation due to DRTs was significantly lower, at 11.4% vs. 28.2% (p = 0.015). The incidence of grade 3/4 transaminase elevation was 20% vs. 29% (p = 0.427), while the incidence of diarrhea was 3% vs. 25% (p = 0.009). The median dose intensity of bosutinib was higher (391.7 mg/day vs. 353.9 mg/day). Pharmacokinetic analysis of bosutinib showed that patients who achieved a major molecular response tended to have high trough concentrations. Thus, a low initial dose of bosutinib followed by dose escalation reduced discontinuation due to severe DRTs while maintaining high dose intensity and efficacy.

Supplementary Information

The online version contains supplementary material available at 10.1007/s12185-024-03830-z.

Keywords

Chronic myeloid leukemia
Bosutinib
Tyrosine kinase inhibitor
Lower dose initiation
Pharmacokinetics
http://dx.doi.org/10.13039/100010793 Pfizer Japan issue-copyright-statement© The Japanese Society of Hematology 2024
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pmcIntroduction

The introduction of ABL1 tyrosine kinase inhibitors (TKIs) has improved survival outcomes for patients with chronic phase chronic myeloid leukemia (CML-CP) markedly [1]. Although imatinib, a pioneering ABL TKI, has many benefits for patients with CML-CP, second-generation TKIs are more effective [2, 3]. Bosutinib is a second-generation ABL TKI approved for the treatment of CML-CP resistant and/or intolerant to prior therapy, as well as for newly diagnosed patients [4–6]. Several clinical trials report the efficacy and safety of bosutinib; however, the drug is associated with drug-related toxicities (DRTs) such as diarrhea or increased transaminase levels, which often lead to treatment discontinuation [6, 7]. The standard initial daily dose of bosutinib is 400 mg for newly diagnosed patients and 500 mg for resistant/intolerant patients, although some experts recommend a lower initiating dose (200–300 mg) to reduce the incidence of adverse events (AEs) [8]; however, the safety and efficacy of lower dose initiation of bosutinib have not been investigated in detail [9]. Moreover, the association between the pharmacokinetics (PK) of low-dose bosutinib and its safety/efficacy are unclear [10, 11].

Hence, we conducted a phase 2 study of BOsutinib Gradual Increase as a second/third-line treatment for CML-CP (BOGI trial, UMIN 000032282) to ascertain whether a lower initiating dose of bosutinib (200 mg daily) would reduce discontinuation rates or treatment interruptions due to DRTs. We also investigated the PK of bosutinib at different doses.

Methods

Study design and patient enrollment

The BOGI trial was a single-arm, multicenter, phase 2 trial conducted across four Japanese hospitals; the aim was to investigate the safety and efficacy of low-dose initiation of bosutinib therapy for patients with CML-CP that was resistant and/or intolerant to prior therapy. Those included were CML-CP patients who developed at least one line of treatment failure (resistant and/or intolerant), were aged ≧18 years, had an Eastern Cooperative Oncology Group performance status score of 0–2, and had adequate organ function (lung, liver, and renal function parameters within normal ranges). Patients newly diagnosed with CML-CP, those with accelerated phase CML or blast phase CML, those who received any other chemotherapy (except hydroxyurea), those with a T315I or V299L BCR::ABL mutation, those with inflammatory bowel disease, those who received cytochrome P-450 CYP3A4 inhibitors or inducers, those infected with human immunodeficiency virus and/or hepatitis B/C virus, those who were pregnant or breastfeeding, and those with critical complications (as defined by the attending physician) were excluded. The trial was approved by the institutional review board of each participating hospital (UMIN 000032282). All procedures involving human participants were undertaken in accordance with the principles of the Declaration of Helsinki, and all participants provided written informed consent. The protocol is available in the appendix (Supplementary appendix).

Procedures

After enrollment, patients received an initial daily dose of bosutinib (200 mg) orally. If no grade 3 or higher AEs occurred, the dose was escalated by 100 mg daily (up to 500 mg daily) every 2 weeks (Supplementary appendix). Major BCR::ABL mRNA levels were measured using a commercially available quantitative reverse transcription PCR (RT-qPCR) kit. Measurements were made at a central laboratory (SRL, Tokyo, Japan or Bio Medical Laboratories, Tokyo, Japan) and in accordance with the standardized international scale (IS). Plasma trough concentrations of bosutinib were measured at inVentive Health Clinical (NJ, USA) using a validated liquid chromatography/tandem mass spectrometry (LC/MS/MS) assay. Major BCR::ABL mRNA levels and plasma trough concentrations of bosutinib were measured every 3 months (i.e., at 3, 6, 9, and 12 months post-initiation). Two or three-color flow cytometry was performed (Bio Medical Laboratories, Tokyo, Japan) at 3, 6, and 12 months post-initiation to assess T cell and natural killer (NK) cell profiles. T cell and natural killer cell subsets were defined as follows: CD8 T cells = CD3+CD8+; NK cells = CD3− CD56+ and CD16+ CD56+; and regulatory T (Treg) cells = CD4+ CD25+ CD127−.

The primary endpoint was the rate of treatment discontinuation at 12 months due to DRTs. Secondary endpoints included the treatment interruption rate, the mean bosutinib dose, number of dosing days and relative dose intensity up until 12 months, achievement of a cytogenetic response (CCyR; equivalent for BCR::ABL1 mRNA ≦ 1.0%) at 6 and 12 months, and the cumulative rates of major molecular response (MMR; BCR::ABL1 mRNA ≦ 0.1%) or deep molecular response (DMR; BCR::ABL1 mRNA ≦ 0.01%) at 3, 6, 9 and 12 months. Sample size was calculated using research and biostatistics Cancer Research and Biostatistics SWOG statistical tools (null proportion = 0.32; alternative proportion = 0.14; one-sided α error = 5%; β error = 20%; power 80%), with the discontinuation rate of the previous Japanese phase 1/2 study of bosutinib as a reference [10]. Considering protocol deviations, the official target enrollment sample size for this trial was 35.

Statistical analysis

For the primary endpoint, the rate of bosutinib discontinuation at 12 months due to DRTs and the one-sided 95% confidence interval (CI) were estimated using the Wilson method. The binomial test was used to evaluate whether the percentage was <32%. For the safety and secondary endpoints, Fisher’s exact test was used for categorical variables and the Mann–Whitney test for continuous variables. The Pearson correlation coefficient is used to determine correlation between bosutinib trough concentrations and several variables. Significant differences between the three groups were assessed using one-way ANOVA (post-hoc tests using Bonferroni), and those between two groups were assessed using the Mann–Whitney U test. The MMR and rate of DMR, as well as the two-sided 95% CI, were also estimated using the Wilson method. Receiver operating characteristic (ROC) curve analysis was done to determine optimal bosutinib concentration. The significance level for the primary hypothesis test was 0.05 (one-sided), and that for all other secondary endpoints was 0.05 (two-sided). Statistical analyses were performed using SAS version 9.4 (SAS Institute Inc., Cary, NC, USA). The study is registered with the UMIN Clinical Trials Registry (UMIN 000032282).

Results

Patient characteristics

Between February 4, 2019 and May 24, 2022, 35 patients from four Japanese hospitals were enrolled. The median age was 61 years (range, 26–81); 19 patients were male and 16 were female, and 16, 12, and seven had low, intermediate, and high Sokal scores, respectively. 11 patients were resistant to prior therapy and 24 were intolerant, respectively. The numbers of patients previously treated with TKIs were as follows: 19 (54%) received one TKI, 12 (34%) received three, and four (11%) received three. Prior TKIs were imatinib (n = 8, 23%), dasatinib (n = 27, 77%), nilotinib (n = 16, 46%), and bosutinib (n = 1, 3%, the patient who received prior treatment with bosutinib was intolerant to standard dosage of bosutinib); 23 of 35 patients (66%) had medical complications, which included hypertension (10, 29%), diabetes mellitus (five, 14%), cardiovascular disease (three, 9%), solid tumors (two, 6%), and cerebrovascular disease (one, 3%). The median major BCR::ABL mRNA level was 0.1% (0.0–34.6%), and serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were 23 U/L (11–63) and 20 U/L (12–94), respectively (Table 1).Table 1 Patient characteristics

	n (%), median (range)	
Age (years)	61 (26–81)	
Sex, male	19 (54)	
Sokal risk	
 Low	16 (46)	
 Intermediate	12 (34)	
 High	7 (20)	
Numbers of prior TKIs	
 One	19 (54)	
 Two	12 (34)	
 Three	4 (11)	
Prior TKIs	
 Imatinib	8 (23)	
 Dasatinib	27 (77)	
 Nilotinib	16 (46)	
 Bosutinib	1 (3)	
BCR::ABL1 mRNA %	0.1 (0–34.6)	
 100–10%	6 (17)	
 10–1.0%	5 (14)	
 1.0–0.1%	6 (17)	
 0.1–0.01%	10 (29)	
 0.01–0.0032%	4 (11)	
 0.0032–0%	4 (11)	
AST (IU/L)	23 (11–63)	
ALT (IU/L)	20 (12–94)	
Complications	23 (66)	
 Hypertension	10 (29)	
 Diabates millitus	5 (14)	
 Cardiovascular disease	3 (9)	
 Solid tumors	2 (6)	
 Cerebrovascular disease	2 (6)	
 Liver disease	0 (0)	
 Autoimmune disorders	0 (0)	
 Others	15 (43)	
Reason of switching to bosutinib	
 Resistant to prior TKI	11 (31)	
 Intolerant to prior TKI	24 (69)	

The bosutinib discontinuation rate due to DRTs was lower than that reported previously

In the intention to treat population, nine of 35 patients had discontinued bosutinib at 12 months; four of these discontinuations were due to DRTs. The total bosutinib discontinuation rate was 25.7% ([95% CI, 15.6–39.3%] vs. 35.9% (14/39) for the Japanese phase 1/2 study [10]; p = 0.102), and the bosutinib discontinuation rate due to DRTs was 11.4% ([95% CI, 5.2–23.2%] vs. 28.2% (11/39, p = 0.015); the differences were significant. Common grade 3 to 4 AEs were consistent with the known safety profiles of bosutinib; these included skin rash (26%), increased AST (9%), and increased ALT (20%); there were no differences in grade 3–4 skin rash (p = 1.00), increased AST (p = 0.690), and increased ALT (p = 0.554) between the present study and the previous the Japanese phase 1/2 study; the incidence of diarrhea was low (3% vs. 25% in the Japanese phase 1/2 study; p = 0.018)). No pleural effusion (0%), cardiovascular events (0%), and only one cerebral hemorrhage (3%), were observed (Table 2). These results suggest that a lower initiating dose of bosutinib reduces the discontinuation rate due to DRTs; thus, the primary endpoint was met. A total of 21 patients (60.0%) interrupted bosutinib at least once due to grade 3 or higher AEs, except for one patient who discontinued due to a grade 2 pleural effusion at the decision of the physician. The other reasons for interruptions were skin rash (n = 9), liver dysfunction (n = 7), diarrhea (n = 1), appetite loss (n = 1), acute enterocolitis (n = 1), increased lipase (n = 1), nausea (n = 1), general fatigue (n = 1), cerebral hemorrhage (n = 1), poisoning rash (n = 1) and anemia (n = 1). Median bosutinib interruption duration was 16 days (range, 6–64). The mean and median bosutinib dose, the number of bosutinib dosing days, and the relative dose intensity up until 12 months were 388.7 (204.9–477.3) mg/day, 391.7 (204.9–477.3) mg, 351.0 (20–365) days, and 78% (41–95), respectively. A swimmer plot detailing treatment duration and outcomes for individuals in the study is shown in Fig. 1.Table 2 Adverse events and laboratory abnormalities during bosutinib treatment (grades 3–4)

	n	%	
Adverse events	20	57	
 Leukocytopenia	0	0	
 Lymphopenia	2	6	
 Neutropenia	1	3	
 Anemia	1	3	
 Thrombocytopenia	0	0	
 Skin rash	9	26	
 Nausea, vomiting	1	3	
 Diarrhea	1	3	
 AST increased	3	9	
 ALT increased	7	20	
 Increased lipase	1	3	
 Acute enterocolitis	1	3	
 Cerebral hemorrhage	1	3	
 Appetite loss	1	3	
 Fatigue	1	3	
 Poisoning rash	1	3	

Fig. 1 Swimmer plot displaying the treatment course. Each bar represents an individual patient

Bosutinib efficacy was high even at the lower initiating dose

BCR::ABL1 mRNA ≦1% was achieved by 28/35 patients (80.0%) at 6 months, and by 26/35 patients (74.3%) at 12 months. Notably, all patients who could continue bosutinib until 12 months achieved BCR::ABL1 mRNA ≦1%, equivalent to a CCyR. The cumulative incidences of MMR and DMR at 12 months were 23/35 (65.7%; 95% CI, 49.2–79.2%) and 15/35 (42.9%; 95% CI, 28.0–59.1%), respectively (Fig. 2A). For those who could be evaluated. The cumulative incidence of a molecular response at each time point is presented in Fig. 2B. Taken together, the results suggest that bosutinib efficacy remains high in CML-CP patients who were resistant and/or intolerant to prior therapy, even when used at a lower initiating dose.Fig. 2 A Major molecular response (MMR) and deep molecular response (DMR) at 12 months. The graph shows the MMR or DMR rates at 12 months after initiation of bosutinib treatment. B Dose distribution for all 35 patients from baseline until 12 months

PK of bosutinib

The average trough concentration of bosutinib at 3, 6 and 12 months was 114.4, 118.0 and 112.6 ng/mL, respectively. When we investigated factors that can affect the bosutinib trough concentration (i.e., patient age, sex, body weight, body mass index, liver function, and renal function), we found that none did (Table S1). Furthermore, there was no difference in average trough concentrations between those who continued (26 patients) and those that discontinued treatment due to AEs (six patients): 116.9 vs. 98.9 ng/mL, respectively (p = 0.409, Mann–Whitney U test; Fig. 3A). With respect to an association between achievement of MMR or DMR and the bosutinib trough concentration, we found that patients who achieved molecular remission tended to have higher trough concentrations: MMR = 117.0 vs. 64.1 ng/mL, respectively (p = 0.210; Fig. 3B) and DMR = 117.7 vs. 104.0 ng/mL, respectively (p = 0.355; Fig. 3C). The optimal trough concentration for a 1 log reduction was 121.0 ng/mL (based on ROC curve analysis and Youden index criteria) (Fig. 3D).Fig. 3 Pharmacokinetics of bosutinib. Trough concentrations of bosutinib in patients who continued treatment (A), or achieved of major molecular response (MMR, B) or a deep molecular response (DMR, C). ROC curve shows that the optimal trough concentration required for a 1 log reduction was 121.0 ng/mL (D). IS, international scale

T cell and NK cell dynamics after initiation of bosutinib treatment

We performed flow cytometry for sequential assessment of changes in the T cell and NK cell profiles following initiation of bosutinib treatment. The percentages of CD8+ T cells, CD3−CD56+ NK cells, CD16+CD56+ NK cells, and Tregs changed little after bosutinib initiation (Figure S1). Next, we evaluated the association between immune cell numbers and bosutinib treatment outcomes. There were no differences in the percentage of CD8+ T cells and NK cells between patients who achieved DMR and those that did not (Fig. 4A–C); however, patients who achieved DMR at 12 months had a lower percentage of Tregs than those who did not (5.7% vs. 7.2%, respectively; p = 0.043; Fig. 4D). These results indicate that bosutinib does not affect most immune cell populations, whereas the low numbers of Tregs might play a role in patient responses to bosutinib.Fig. 4 Flow cytometry analysis of T cells and natural killer (NK) cells at 12 months post-treatment initiation. Percentage of CD3+ T cells (A), CD16+CD56+ NK cells (B), CD3−CD56+NK cells (C), and regulatory T cells according to the molecular response (D). DMR, deep molecular response

Discussion

International prospective clinical trials show that bosutinib is a safe and effective second-generation TKI for patients with newly diagnosed or pre-treatment refractory-intolerant CML-CP [4–7], although DRT forces dose reduction in some patients; however, sub-analyses indicate an adequate molecular response in such patients [12, 13]. Establishing the optimal dose of bosutinib is necessary to achieve optimal efficacy while avoiding AEs; therefore, some experts recommend a lower initiating dose [8].

Mita et al. [14] conducted a retrospective study comparing a dose-escalation regimen of bosutinib versus a standard dose starting regimen. The initiating dose of bosutinib was 100 mg daily, escalating to 500 mg daily; the result was a reduction in interruption or discontinuation rates due to AEs when compared with the standard 500 mg daily fixed dose [14]. The recent BODO study, a phase 2 trial examining the safety and efficacy of step-in dosing of bosutinib [9], reported that a 300 mg daily initiation regimen failed to reduce gastrointestinal AEs or discontinuation rates; 17 of the 57 patients (29.8%) discontinued bosutinib due to AEs [9]. In the present BOGI trial, the initial dose of bosutinib was 200 mg daily; subsequently, the dose was escalated by 100 mg daily (up to 500 mg daily) every 2 weeks. The primary endpoint was the bosutinib discontinuation rate due to DRTs at 12 months; the result was 11.4%; hence, the primary endpoint was met. The lower rate in the BOGI trial compared with that in the Japanese phase 1/2 study [10] was attributed to an apparent reduction in the incidence of diarrhea. Furthermore, a previous study reported a correlation between the average bosutinib concentration during the early treatment phase (< 1 week) and occurrence of grade 3 diarrhea, suggesting that a lower initiating dose of bosutinib reduce diarrhea and therefore AEs [15]. These results suggest that a 300 mg daily initiating dose may be too high for a certain subset of patients, whereas 100 to 200 mg daily may be optimal [14, 16].

Approximately 10–20% of patients who receive bosutinib developed a grade 3 to 4 increase in transaminase levels, leading to discontinuation [4, 6, 7]; grade 3 to 4 increases in transaminase are more common in Japanese patients than in non-Japanese patients (36.2 vs. 17.7%, respectively); this leads directly to bosutinib discontinuation in 30.4% of Japanese patients [17]. In the BOGI trial, we noted a similar frequency of grade 3 to 4 increases in transaminase (AST, 9%; ALT, 20%) [4, 6, 7], although these did not lead to bosutinib discontinuation. Since it is not uncommon for initial elevations in transaminase to resolve spontaneously, close monitoring and early evaluation of liver enzymes, along with appropriate modifications in the drug dose, might have helped these patients to continue bosutinib throughout the BOGI trial. Furthermore, the median dose of bosutinib in patients enrolled in the BOGI trial was relatively higher (391.7 mg/day) than that in the previous Japanese clinical study (353.9 mg/day) [17], although the cumulative incidences of MMR and DMR at 12 months (MMR, 65.7%; DMR, 42.9%) were comparable with those in the BELA or BYOND trials [4, 7]; therefore, a lower initiating dose (followed by subsequent dose escalation) regimen can maintain dose intensity, resulting in an adequate therapeutic effect. Since the continuation rates and efficacy of bosutinib are associated [4, 6, 7, 18], frequent monitoring of liver enzyme along with dose modification is important to avoid bosutinib discontinuation due to increased transaminase levels while at the same time enabling optimal efficacy. Although the observation period was short, the low incidence of cardiovascular AEs in the BOGI trial was notable.

We also measured trough concentrations of bosutinib over time to identify the effective therapeutic range that exhibits optimal safety and efficacy. Unfortunately, we found no significant difference in the trough concentrations between patients who discontinued bosutinib due to AEs (n = 6) and those who were able to continue (n = 26). This may be due to the fact that few patients had their concentrations measured at the time of AE onset and/or at the time of bosutinib discontinuation. Only six of nine patients who discontinued bosutinib due to AEs had bosutinib concentrations measured at the time determined by the protocol. Bosutinib trough concentrations are associated with gastrointestinal toxicity; however, the target trough concentration needed to avoid AEs remains unclear [15]. Patients who achieved an MMR tended to have higher trough concentrations, a finding consistent with a previous report; the optimal trough concentration for a 1 log reduction in the BOGI trial was 121.0 ng/mL. Further PK studies are needed to establish target concentrations and the optimal individual doses require to ensure safety and efficacy.

Several lines of evidence suggest that CML is sensitive to immunotherapy [19], and that responses to TKI therapy can be determined not only by direct binding capacity for BCR::ABL but also by anti-tumor immune responses [20, 21]. Imatinib or dasatinib often increase NK cell numbers or decrease Treg numbers, leading to favorable clinical responses [22–24], while nilotinib or bosutinib affect immune cells only weakly [23]. Tregs, which are major mediators of mammalian self-tolerance, suppress anti-tumor immunity, making them obstacles to successful cancer treatment [25]. In the present study, we found that bosutinib did not affect T and NK cells, as reported previously [23, 26]; however, we found that the outcome of bosutinib treatment might be dependent on the number of Tregs. Lower Treg numbers are associated with successful achievement of a DMR [24, 27], as well as maintenance of a treatment free remission in CML patients because they activate anti-CML immune responses [28]. These results suggest that although bosutinib itself might have no direct effect on immune cells, immune cells may affect the therapeutic efficacy of bosutinib.

Conclusion

A lower initiating dose of bosutinib reduces drug discontinuation rates, or interruption of treatment, due to severe DRTs, particularly diarrhea, while maintaining therapeutic efficacy. Although bosutinib concentration levels might correlate with safety and efficacy, the target trough concentrations required for optimal safety and efficacy remain unclear. Further large-scale studies are needed to further investigate the PK of bosutinib.

Supplementary Information

Below is the link to the electronic supplementary material.Supplementary file1 (DOCX 17 KB)

Supplementary file2 (PDF 209 KB)

Supplementary file3 (DOCX 165 KB)

Acknowledgements

This study was funded by Pfizer Japan Inc., Tokyo, Japan. T. Ikezoe is an editor of International Journal of Hematology.

Author contributions

N.T. and Shinya. K. made substantial contributions to study conception, design, and data analysis and interpretation. N.T., Ikezoe T., Y.K., Satoshi K, N.F., Ichinohe T., and Shinya K. enrolled patients and collected data. M.M. performed pharmacokinetics analysis. A.T. and A.K. collected data and performed statistical analyses. H.U., N.T., A.T. and A.K. wrote the paper. H.U., N.T. and Shinya K. critically reviewed the drafts. All authors approved the final version.

Data availability

All data can be assessed by contacting the corresponding author (NT).

Declarations

Conflict of interest

NT declares personal fees from Novartis, Pfizer Inc., and Otsuka Pharmaceutical Co., Ltd. T. Ikezoe received honoraria from Pfizer, Otsuka Pharmaceuticals and Novartis, and research funding from Pfizer, Novartis and Otsuka Pharmaceuticals. T. Ichinohe received honoraria from Novaritis. Shinya Kimura received honoraria from Pfizer, Otsuka Pharmaceuticals, Novartis and Bristol-Myers-Squibb, and research funding from Pfizer, Bristol-Myers-Squibb and Ohara Pharmaceuticals. All other authors declare no competing interests.

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