
==== Front
Wellcome Open Res
Wellcome Open Res
Wellcome Open Research
2398-502X
F1000 Research Limited London, UK

10.12688/wellcomeopenres.22425.1
Data Note
Articles
The genome sequence of the oak pinhole borer, Platypus cylindrus Fabricius, 1792
[version 1; peer review: 2 approved, 1 approved with reservations]

Barclay Maxwell V. L. Investigation Resources https://orcid.org/0000-0003-4989-2014
1
Vassiliades Danaë Investigation Resources 1
Bayfield Farrell Will Investigation Resources https://orcid.org/0000-0002-1127-5072
1
Cristóvão Joana Investigation Resources 1
Matsumoto Keita Investigation Resources 1
Geiser Michael Investigation Resources 1
Telfer Mark G. Investigation Resources 2
Natural History Museum Genome Acquisition Lab
University of Oxford and Wytham Woods Genome Acquisition Lab
Darwin Tree of Life Barcoding collective
Wellcome Sanger Institute Tree of Life Management, Samples and Laboratory team
Wellcome Sanger Institute Scientific Operations: Sequencing Operations
Wellcome Sanger Institute Tree of Life Core Informatics team
Tree of Life Core Informatics collective
Darwin Tree of Life Consortiuma
1 Natural History Museum, London, England, UK
2 Independent researcher, Ventnor, Isle of Wight, England, UK
a mark.blaxter@sanger.ac.uk
No competing interests were disclosed.

11 6 2024
2024
9 30516 5 2024
Copyright: © 2024 Barclay MVL et al.
2024
https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

We present a genome assembly from an individual male Platypus cylindrus (the oak pinhole borer; Arthropoda; Insecta; Coleoptera; Curculionidae). The genome sequence is 147.5 megabases in span. Most of the assembly is scaffolded into 8 chromosomal pseudomolecules, including the X and Y sex chromosomes. The mitochondrial genome has also been assembled and is 19.29 kilobases in length. Gene annotation of this assembly on Ensembl identified 13,468 protein coding genes.

Platypus cylindrus
oak pinhole borer
genome sequence
chromosomal
Coleoptera
Wellcome Trust206194 218328 This work was supported by Wellcome through core funding to the Wellcome Sanger Institute [206194, <a href=https://doi.org/10.35802/206194>https://doi.org/10.35802/206194</a>] and the Darwin Tree of Life Discretionary Award [218328, <a href=https://doi.org/10.35802/218328>https://doi.org/10.35802/218328 </a>]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
==== Body
pmcSpecies taxonomy

Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Coleoptera; Polyphaga; Cucujiformia; Curculionoidea; Curculionidae; Platypodinae; Platypus; Platypus cylindrus Fabricius, 1792 (NCBI:txid298138).

Background

The genome of the oak pinhole borer, Platypus cylindrus, was sequenced as part of the Darwin Tree of Life Project, a collaborative effort to sequence all named eukaryotic species in the Atlantic Archipelago of Britain and Ireland. Here we present a chromosomally complete genome sequence for Platypus cylindrus, based on one male specimen from Bookham Commons, England, UK.

Genome sequence report

The genome was sequenced from one male Platypus cylindrus ( Figure 1) collected from Bookham Commons, England, UK (51.29, –0.39). A total of 181-fold coverage in Pacific Biosciences single-molecule HiFi long reads was generated. Primary assembly contigs were scaffolded with chromosome conformation Hi-C data. Manual assembly curation corrected 34 missing joins or mis-joins and removed 4 haplotypic duplications, reducing the assembly length by 0.38% and the scaffold number by 16.13%, and increasing the scaffold N50 by 6.57%.

Figure 1. Photographs of the Platypus cylindrus (icPlaCyli4) specimen used for genome sequencing: a) dorsal view, b) lateral view, c) ventral view.

The final assembly has a total length of 147.5 Mb in 25 sequence scaffolds with a scaffold N50 of 15.2 Mb ( Table 1). The snailplot in Figure 2 provides a summary of the assembly statistics, while the distribution of assembly scaffolds on GC proportion and coverage is shown in Figure 3. The cumulative assembly plot in Figure 4 shows curves for subsets of scaffolds assigned to different phyla. Most (96.77%) of the assembly sequence was assigned to 8 chromosomal-level scaffolds, representing 6 autosomes and the X and Y sex chromosomes. Chromosome-scale scaffolds confirmed by the Hi-C data are named in order of size ( Figure 5; Table 2). Y chromosome scaffolds were identified but were not scaffolded, as Hi-C data are from a female sample. While not fully phased, the assembly deposited is of one haplotype. Contigs corresponding to the second haplotype have also been deposited. The mitochondrial genome was also assembled and can be found as a contig within the multifasta file of the genome submission.

Table 1. Genome data for Platypus cylindrus, icPlaCyli4.1.

Project accession data	
Assembly identifier	icPlaCyli4.1	
Species	Platypus cylindrus	
Specimen	icPlaCyli4	
NCBI taxonomy ID	298138	
BioProject	PRJEB59384	
BioSample ID	SAMEA110019313	
Isolate information	icPlaCyli4, male (DNA sequencing)
icPlaCyli3, female (Hi-C sequencing)	
Assembly metrics *	Benchmark	
Consensus quality (QV)	62.7	≥ 50	
k-mer completeness	100.0%	≥ 95%	
BUSCO **	C:96.2%[S:95.0%,D:1.2%],F:1.2%,M:2.6%,n:2,124	C ≥ 95%	
Percentage of assembly mapped to chromosomes	96.77%	≥ 95%	
Sex chromosomes	XY	localised homologous pairs	
Organelles	Mitochondrial genome: 19.29 kb	complete single alleles	
Raw data accessions	
PacificBiosciences SEQUEL II	ERR10841325	
Hi-C Illumina	ERR10851522	
Genome assembly	
Assembly accession	GCA_949748235.1	
Accession of alternate haplotype	GCA_949748395.1	
Span (Mb)	147.5	
Number of contigs	128	
Contig N50 length (Mb)	2.4	
Number of scaffolds	25	
Scaffold N50 length (Mb)	15.2	
Longest scaffold (Mb)	53.44	
Genome annotation	
Number of protein-coding genes	13,468	
Number of gene transcripts	13,768	
* Assembly metric benchmarks are adapted from column VGP-2020 of “Table 1: Proposed standards and metrics for defining genome assembly quality” from Rhie et al. (2021).

** BUSCO scores based on the endopterygota_odb10 BUSCO set using version 5.3.2. C = complete [S = single copy, D = duplicated], F = fragmented, M = missing, n = number of orthologues in comparison. A full set of BUSCO scores is available at https://blobtoolkit.genomehubs.org/view/icPlaCyli4_1/dataset/icPlaCyli4_1/busco.

Figure 2. Genome assembly of Platypus cylindrus, icPlaCyli4.1: metrics.

The BlobToolKit Snailplot shows N50 metrics and BUSCO gene completeness. The main plot is divided into 1,000 size-ordered bins around the circumference with each bin representing 0.1% of the 147,483,955 bp assembly. The distribution of scaffold lengths is shown in dark grey with the plot radius scaled to the longest scaffold present in the assembly (53,441,370 bp, shown in red). Orange and pale-orange arcs show the N50 and N90 scaffold lengths (15,234,454 and 11,925,517 bp), respectively. The pale grey spiral shows the cumulative scaffold count on a log scale with white scale lines showing successive orders of magnitude. The blue and pale-blue area around the outside of the plot shows the distribution of GC, AT and N percentages in the same bins as the inner plot. A summary of complete, fragmented, duplicated and missing BUSCO genes in the endopterygota_odb10 set is shown in the top right. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/icPlaCyli4_1/dataset/icPlaCyli4_1/snail.

Figure 3. Genome assembly of Platypus cylindrus, icPlaCyli4.1: BlobToolKit GC-coverage plot.

Sequences are coloured by phylum. Circles are sized in proportion to sequence length. Histograms show the distribution of sequence length sum along each axis. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/icPlaCyli4_1/dataset/icPlaCyli4_1/blob.

Figure 4. Genome assembly of Platypus cylindrus, icPlaCyli4.1: BlobToolKit cumulative sequence plot.

The grey line shows cumulative length for all sequences. Coloured lines show cumulative lengths of sequences assigned to each phylum using the buscogenes taxrule. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/icPlaCyli4_1/dataset/icPlaCyli4_1/cumulative.

Figure 5. Genome assembly of Platypus cylindrus, icPlaCyli4.1: Hi-C contact map of the icPlaCyli4.1 assembly, visualised using HiGlass.

Chromosomes are shown in order of size from left to right and top to bottom. An interactive version of this figure may be viewed at https://genome-note-higlass.tol.sanger.ac.uk/l/?d=b-vYOkPgQiCy-EjqgJSJdQ.

Table 2. Chromosomal pseudomolecules in the genome assembly of Platypus cylindrus, icPlaCyli4.

INSDC accession	Chromosome	Length (Mb)	GC%	
OX456488.1	1	53.44	31.0	
OX456489.1	2	19.33	30.5	
OX456490.1	3	15.23	31.5	
OX456491.1	4	12.45	30.5	
OX456493.1	5	12.27	31.5	
OX456494.1	6	11.93	30.5	
OX456492.1	X	12.28	31.0	
OX456495.1	Y	2.77	31.0	
OX456496.1	MT	0.02	20.5	

The estimated Quality Value (QV) of the final assembly is 62.7 with k-mer completeness of 100.0%, and the assembly has a BUSCO v5.3.2 completeness of 96.2% (single = 95.0%, duplicated = 1.2%), using the endopterygota_odb10 reference set ( n = 2,124).

Metadata for specimens, barcode results, spectra estimates, sequencing runs, contaminants and pre-curation assembly statistics are given at https://links.tol.sanger.ac.uk/species/298138.

Genome annotation report

The Platypus cylindrus genome assembly (GCA_949748235.1) was annotated using the Ensembl rapid annotation pipeline ( Table 1; https://rapid.ensembl.org/Platypus_cylindrus_GCA_949748235.1/Info/Index). The resulting annotation includes 13,768 transcribed mRNAs from 13,468 protein-coding genes.

Methods

Sample acquisition and nucleic acid extraction

The specimen used for genome sequencing, a male Platypus cylindrus (specimen ID NHMUK014439781, ToLID icPlaCyli4), was collected from Bookham Commons, England, UK (latitude 51.29, longitude –0.39) on 2021-09-19. The specimen was collected by Maxwell Barclay, Michael Geiser, Danaë Vassiliades, Will Bayfield Farrell and Joana Cristovao (Natural History Museum) and identified by Maxwell Barclay (Natural History Museum), and then preserved by dry freezing at –80°C.

The specimen used for Hi-C sequencing was a female Platypus cylindrus (specimen ID Ox001653, ToLID icPlaCyli3) was collected from Wytham Woods, Oxfordshire (biological vice-county Berkshire), UK (latitude 51.77, longitude –1.33) on 2021-07-08. The specimen was collected and identified by Mark Telfer (independent researcher) and preserved on dry ice.

The workflow for high molecular weight (HMW) DNA extraction at the Wellcome Sanger Institute (WSI) includes a sequence of core procedures: sample preparation; sample homogenisation, DNA extraction, fragmentation, and clean-up. In sample preparation, the icPlaCyli4 sample was weighed and dissected on dry ice ( Jay et al., 2023). Tissue from the head and thorax was homogenised using a PowerMasher II tissue disruptor ( Denton et al., 2023a).

HMW DNA was extracted in the WSI Scientific Operations core using the Automated MagAttract v2 protocol ( Oatley et al., 2023). The DNA was sheared into an average fragment size of 12–20 kb in a Megaruptor 3 system with speed setting 31 ( Bates et al., 2023). Sheared DNA was purified by solid-phase reversible immobilisation ( Strickland et al., 2023): in brief, the method employs a 1.8X ratio of AMPure PB beads to sample to eliminate shorter fragments and concentrate the DNA. The concentration of the sheared and purified DNA was assessed using a Nanodrop spectrophotometer and Qubit Fluorometer and Qubit dsDNA High Sensitivity Assay kit. Fragment size distribution was evaluated by running the sample on the FemtoPulse system.

Protocols developed by the WSI Tree of Life laboratory are publicly available on protocols.io ( Denton et al., 2023b).

Sequencing

Pacific Biosciences HiFi circular consensus DNA sequencing libraries were constructed according to the manufacturers’ instructions. DNA sequencing was performed by the Scientific Operations core at the WSI on a Pacific Biosciences SEQUEL II instrument. Hi-C data were also generated from whole organism tissue of icPlaCyli3 using the Arima2 kit and sequenced on the Illumina NovaSeq 6000 instrument.

Genome assembly, curation and evaluation

Assembly was carried out with Hifiasm ( Cheng et al., 2021) and haplotypic duplication was identified and removed with purge_dups ( Guan et al., 2020). The assembly was then scaffolded with Hi-C data ( Rao et al., 2014) using YaHS ( Zhou et al., 2023). The assembly was checked for contamination and corrected using the gEVAL system ( Chow et al., 2016) as described previously ( Howe et al., 2021). Manual curation was performed using gEVAL, HiGlass ( Kerpedjiev et al., 2018) and Pretextview ( Harry, 2022). The mitochondrial genome was assembled using MitoHiFi ( Uliano-Silva et al., 2023), which runs MitoFinder ( Allio et al., 2020) or MITOS ( Bernt et al., 2013) and uses these annotations to select the final mitochondrial contig and to ensure the general quality of the sequence.

A Hi-C map for the final assembly was produced using bwa-mem2 ( Vasimuddin et al., 2019) in the Cooler file format ( Abdennur & Mirny, 2020). To assess the assembly metrics, the k-mer completeness and QV consensus quality values were calculated in Merqury ( Rhie et al., 2020). This work was done using Nextflow ( Di Tommaso et al., 2017) DSL2 pipelines “sanger-tol/readmapping” ( Surana et al., 2023a) and “sanger-tol/genomenote” ( Surana et al., 2023b). The genome was analysed within the BlobToolKit environment ( Challis et al., 2020) and BUSCO scores ( Manni et al., 2021; Simão et al., 2015) were calculated.

Table 3 contains a list of relevant software tool versions and sources.

Table 3. Software tools: versions and sources.

Software tool	Version	Source	
BlobToolKit	4.2.1	https://github.com/blobtoolkit/blobtoolkit	
BUSCO	5.3.2	https://gitlab.com/ezlab/busco	
gEVAL	N/A	https://geval.org.uk/	
Hifiasm	0.16.1-r375	https://github.com/chhylp123/hifiasm	
HiGlass	1.11.6	https://github.com/higlass/higlass	
Merqury	MerquryFK	https://github.com/thegenemyers/MERQURY.FK	
MitoHiFi	2	https://github.com/marcelauliano/MitoHiFi	
PretextView	0.2	https://github.com/wtsi-hpag/PretextView	
purge_dups	1.2.3	https://github.com/dfguan/purge_dups	
sanger-tol/genomenote	v1.0	https://github.com/sanger-tol/genomenote	
sanger-tol/readmapping	1.1.0	https://github.com/sanger-tol/readmapping/tree/1.1.0	
YaHS	1.2a	https://github.com/c-zhou/yahs	

Genome annotation

The BRAKER2 pipeline ( Brůna et al., 2021) was used in the default protein mode to generate annotation for the Platypus cylindrus assembly (GCA_949748235.1) in Ensembl Rapid Release.

Wellcome Sanger Institute – Legal and Governance

The materials that have contributed to this genome note have been supplied by a Darwin Tree of Life Partner. The submission of materials by a Darwin Tree of Life Partner is subject to the ‘Darwin Tree of Life Project Sampling Code of Practice’, which can be found in full on the Darwin Tree of Life website here. By agreeing with and signing up to the Sampling Code of Practice, the Darwin Tree of Life Partner agrees they will meet the legal and ethical requirements and standards set out within this document in respect of all samples acquired for, and supplied to, the Darwin Tree of Life Project.

Further, the Wellcome Sanger Institute employs a process whereby due diligence is carried out proportionate to the nature of the materials themselves, and the circumstances under which they have been/are to be collected and provided for use. The purpose of this is to address and mitigate any potential legal and/or ethical implications of receipt and use of the materials as part of the research project, and to ensure that in doing so we align with best practice wherever possible. The overarching areas of consideration are:

•     Ethical review of provenance and sourcing of the material

•     Legality of collection, transfer and use (national and international)

Each transfer of samples is further undertaken according to a Research Collaboration Agreement or Material Transfer Agreement entered into by the Darwin Tree of Life Partner, Genome Research Limited (operating as the Wellcome Sanger Institute), and in some circumstances other Darwin Tree of Life collaborators.

Data availability

European Nucleotide Archive: Platypus cylindrus. Accession number PRJEB59384; https://identifiers.org/ena.embl/PRJEB59384 ( Wellcome Sanger Institute, 2023). The genome sequence is released openly for reuse. The Platypus cylindrus genome sequencing initiative is part of the Darwin Tree of Life (DToL) project. All raw sequence data and the assembly have been deposited in INSDC databases. Raw data and assembly accession identifiers are reported in Table 1.

Author information

Members of the Natural History Museum Genome Acquisition Lab are listed here: https://doi.org/10.5281/zenodo.7139035.

Members of the University of Oxford and Wytham Woods Genome Acquisition Lab are listed here: https://doi.org/10.5281/zenodo.7125292.

Members of the Darwin Tree of Life Barcoding collective are listed here: https://doi.org/10.5281/zenodo.4893703.

Members of the Wellcome Sanger Institute Tree of Life Management, Samples and Laboratory team are listed here: https://doi.org/10.5281/zenodo.10066175.

Members of Wellcome Sanger Institute Scientific Operations: Sequencing Operations are listed here: https://doi.org/10.5281/zenodo.10043364.

Members of the Wellcome Sanger Institute Tree of Life Core Informatics team are listed here: https://doi.org/10.5281/zenodo.10066637.

Members of the Tree of Life Core Informatics collective are listed here: https://doi.org/10.5281/zenodo.5013541.

Members of the Darwin Tree of Life Consortium are listed here: https://doi.org/10.5281/zenodo.4783558.

10.21956/wellcomeopenres.24704.r95872
Reviewer response for version 1
Palmieri Luciano 1Referee https://orcid.org/0000-0002-4813-2614

1 Competence Centre for Plant Health, Free University of Bozen-Bolzano, Bolzano, BZ, Italy
18 9 2024 Copyright: © 2024 Palmieri L
2024
https://creativecommons.org/licenses/by/4.0/ This is an open access peer review report distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Version 1recommendationapprove
This manuscript presents the genome assembly of Platypus cylindrus, as part of the Darwin Tree of Life Project, which aims to sequence all named eukaryotic species in Britain and Ireland. The assembled genome spans 147.5 Mbases, with most of the sequence scaffolded into 8 chromosomal pseudomolecules, including the X and Y sex chromosomes. Additionally, the mitochondrial genome (19.29 Kbases) was successfully assembled, and automatic gene annotation identified 13,468 protein-coding genes. The genome was sequenced using PacBio HiFi long reads from a male specimen, and scaffolded with Hi-C data. The assembly is of high quality, with a BUSCO completeness score of 96.2%.

This study is part of a growing body of work from the same group, which has developed and refined an efficient pipeline for generating high-quality genome assemblies. This pipeline has been used to describe the genomes of dozens of insect species, making significant contributions to our understanding of insect  genomics. The rationale for creating this dataset is not often clear, while it provides valuable data for understanding the genetics of P. cylindrus, the broader ecological and evolutionary context of this species is less emphasized. Nonetheless, the described genome provides a foundational resource for further research into the genetics, evolution, and ecological role of this species.

Are sufficient details of methods and materials provided to allow replication by others?

Yes

Is the rationale for creating the dataset(s) clearly described?

Partly

Are the datasets clearly presented in a useable and accessible format?

Yes

Are the protocols appropriate and is the work technically sound?

Yes

Reviewer Expertise:

ColeopteraSystematicsBiogeographyBiodiversityTaxonomyEntomologySystematic EntomologyInsect TaxonomyPhylogenetic AnalysisEvolutionInsect IdentificationNatural HistoryScientific Digital PhotographySpecies DiversityPhylogeneticsMolecular PhylogeneticsMolecular TaxonomyMolecular SystematicsInvertebrate ZoologyZoological NomenclatureFicusParasitoidsMacroevolutionGenome AssemblyPhylogenomics

I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard.

10.21956/wellcomeopenres.24704.r95869
Reviewer response for version 1
Tandonnet Sophie 1Referee https://orcid.org/0000-0001-7559-0154

1 Universitat de Barcelona, Barcelona, Catalonia, Spain
4 9 2024 Copyright: © 2024 Tandonnet S
2024
https://creativecommons.org/licenses/by/4.0/ This is an open access peer review report distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Version 1recommendationapprove-with-reservations
In this note, the authors present a highly complete and continuous genome assembly for the species Platypus cylindrus. It is an addition to the growing database of high quality genomes coming from the Darwin Tree of Life project. The note is well written and the data correctly referenced and available. The annotation is available containing 13,468 protein coding genes.

Below are some suggestions, questions and general comments minor comment: I missed reading more background on this species: ecology, special characteristics, lifestyle, habitat, etc…

The abstract states that "Most of the assembly is scaffolded into 8 chromosomal pseudomolecules, including the X and Y sex chromosomes." but later in the report it is said "Y chromosome scaffolds were identified but were not scaffolded". Looking at the PRJEB60808 project page on ENA, I did find the Y chromosome as a single sequence. So I'm confused: is the Y chromosome on ENA only a part of the Y? Or were all the scaffolds tagged as Y collated together?  Could the authors clarify this point?

Related to the previous point: how were the X and Y chromosome material identified? Was a coverage analysis performed? Was it based on synteny? If so, with which species? I think it is very cool to have the Y chromosome (which is usually hard to assemble well) and it may be useful for other researchers to have more information on how the Y was identified.

Add "from an additional female specimen" in the sentence "Primary assembly contigs were scaffolded with chromosome conformation Hi-C data." in the report section. This is because other assemblies have both the HiC and the Pacbio/10X sequencing from the same individual.

For the genome annotation: please add the protein database or transcriptome data used as this can influence the completeness of the annotation. Is 13,468 protein coding genes around the expected number of genes for a beetle? What is the proteome BUSCO score?

The PRJEB59383 page states that "This project collects the genomic and transcriptomic data generated for Platypus cylindrus". However I couldn't locate the transcriptomic data. 

The MT genome is 19.29 kilobases in length: did the authors check for internal duplication in this sequence?

Figure 5: Why are there "white bands" (no contacts) in the Hi-C contact map?

Sample acquisition and nucleic acid extraction: the text seems to relate to the handling of the male sample. How was the female sample handled for HiC data generation?

Are sufficient details of methods and materials provided to allow replication by others?

Partly

Is the rationale for creating the dataset(s) clearly described?

Yes

Are the datasets clearly presented in a useable and accessible format?

Yes

Are the protocols appropriate and is the work technically sound?

Yes

Reviewer Expertise:

Evolutionary Biology, Genetics, sex determination, bioinformatics, non-model organisms

I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above.

10.21956/wellcomeopenres.24704.r89813
Reviewer response for version 1
Angus Robert 1Referee
1 Department of Life Sciences (Insects), The Natural History Museum, London, UK
26 7 2024 Copyright: © 2024 Angus R
2024
https://creativecommons.org/licenses/by/4.0/ This is an open access peer review report distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Version 1recommendationapprove
These authors present this piece of work as part of the Natural History Museum’s project to accumulate DNA genotypes of all British animals. As such it is a valuable contribution, based on a recently captured reliably identified beetle. It is a straightforward account using their standard protocols.

A sound and useful piece of work.

Are sufficient details of methods and materials provided to allow replication by others?

Yes

Is the rationale for creating the dataset(s) clearly described?

Yes

Are the datasets clearly presented in a useable and accessible format?

Yes

Are the protocols appropriate and is the work technically sound?

Yes

Reviewer Expertise:

NA

I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard.

Competing interests: No competing interests were disclosed.

Competing interests: No competing interests were disclosed.

Competing interests: No competing interests were disclosed.
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