
==== Front
bioRxiv
BIORXIV
bioRxiv
2692-8205
Cold Spring Harbor Laboratory

10.1101/2024.08.29.610390
preprint
1
Article
Validation of 3D cryoEM single particle reconstruction correctness and handedness with Ewald’s sphere correction
Bromberg Raquel http://orcid.org/0000-0003-3526-6362

Guo Yirui http://orcid.org/0000-0001-7705-5454

Borek Dominika http://orcid.org/0000-0002-4321-6253

Otwinowski Zbyszek http://orcid.org/0000-0003-3640-8545

30 8 2024
2024.08.29.610390https://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License, which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.
http://biorxiv.org/lookup/doi/10.1101/2024.08.29.610390
nihpp-2024.08.29.610390.pdf
Abstract

The correct description of quantum scattering places the observed scattering contributions on the Ewald’s sphere and its Friedel mate. In electron microscopy, due to the large radius of the Ewald’s sphere, these contributions are typically merged during data analysis. We present an approach that separates and factorizes these contributions into inversion-symmetric and inversion-antisymmetric components. The correlations between reconstructions derived from these symmetric and antisymmetric components enable the automatic determination of handedness and provide additional validation for the quality of 3D reconstructions. These correlations are robust enough to be routinely used in single-particle reconstructions, even at resolutions below the limit where the curvature of the Ewald’s sphere affects the overall signal-to-noise ratio.

Synopsis

The implementation of Ewald’s sphere correction that separates the chirality-sensitive component provides handedness determination and validation of 3D reconstruction.
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pmc
