
==== Front
Open Forum Infect Dis
Open Forum Infect Dis
ofid
Open Forum Infectious Diseases
2328-8957
Oxford University Press US

38798899
10.1093/ofid/ofae250
ofae250
Diagnostic Methods and Tools
Brief Report
AcademicSubjects/MED00290
Reactivation of Multidrug-Resistant HSV-1 in a Post–Allogenic Hematopoietic Stem Cell Transplant Patient: Dynamic Detection of the Rare A605V Mutation by Next-Generation Sequencing
https://orcid.org/0000-0003-1176-2601
Zheng Shuxuan Department of Medical Microbiology, UMC Utrecht, Utrecht, The Netherlands

Rümke Lidewij L W Department of Medical Microbiology, UMC Utrecht, Utrecht, The Netherlands

Tello Rubio Bruno Department of Medical Microbiology, UMC Utrecht, Utrecht, The Netherlands

Rogers Malbert R C Department of Medical Microbiology, UMC Utrecht, Utrecht, The Netherlands

van Sluis Geerte L Department of Haematology UMC Utrecht, Utrecht, The Netherlands

Kuball Jürgen H E Department of Haematology UMC Utrecht, Utrecht, The Netherlands

Riezebos-Brilman Annelies Department of Medical Microbiology, UMC Utrecht, Utrecht, The Netherlands

Lebbink Robert Jan Department of Medical Microbiology, UMC Utrecht, Utrecht, The Netherlands

https://orcid.org/0000-0002-9787-1827
Verduyn Lunel Frans M Department of Medical Microbiology, UMC Utrecht, Utrecht, The Netherlands

Shuxuan Zheng and Lidewij L. W. Rümke equal contribution

Correspondence: Frans M. Verduyn Lunel, MD, PhD, Department of Medical Microbiology, UMC Utrecht, Utrecht, The Netherlands (f.m.verduynlunel@umcutrecht.nl).
Potential conflicts of interest. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

5 2024
03 5 2024
03 5 2024
11 5 ofae25001 2 2024
26 4 2024
01 5 2024
25 5 2024
© The Author(s) 2024. Published by Oxford University Press on behalf of Infectious Diseases Society of America.
2024
https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We present an immunocompromised patient with a multiresistant herpes simplex virus–1 reactivation with a rare mutation (A605V) in the viral DNA polymerase gene. Next-generation sequencing suggests the presence of multiple drug-resistant strains before treatment and altered ratios during treatment, affecting the clinical response to aciclovir and foscarnet.

aciclovir
foscarnet
HSV-1
multidrug resistance
stem cell transplantation
==== Body
pmcHematopoietic stem cell transplant (HSCT) recipients face a substantial risk of severe herpes simplex virus (HSV) reactivation [1]. Drugs of choice for prophylaxis and therapy are nucleoside analogues aciclovir (ACV) and its prodrug valaciclovir (ValACV) [2]. Resistance to ACV and ValACV often coincides with cross-resistance to other nucleoside analogues such as penciclovir and its prodrug famciclovir [3]. In infections refractory to nucleoside analogues, other therapeutic options are the more toxic agents: foscarnet (FOS; a pyrophosphate analogue) and cidofovir (CDV; a nucleotide analogue) [2, 4]. Resistance to HSV antivirals can be attributed to changes in the viral enzyme thymidine kinase (TK; encoded by the UL23 gene) and viral DNA polymerase (DNA pol, encoded by the UL30 gene) [4, 5]. To exert activity, ACV first needs to be phosphorylated by viral TK and cellular kinases before targeting DNA pol, whereas FOS directly inhibits viral DNA pol (UL30) [6]. In contrast to TK, functional DNA pol is essential for viral replication, and therefore DNA pol mutations in clinical isolates rarely occur [4, 6, 7]. Drug-resistant mutations in HSV-1 can be identified at the genetic level using various techniques, including conventional Sanger sequencing and, more recently, next-generation sequencing (NGS) [2, 8]. The latter has the additional advantage of enhanced sensitivity to detect minor variants in mixed populations [8]. In this study, we present an HSCT patient experiencing reactivation of HSV-1 that is resistant to ACV and FOS treatment. This resistance was attributed to a mutation in the TK gene (R281STOP, associated with ACV resistance) as well as a less common mutation in the DNA pol gene (A605V, associated with ACV and FOS resistance). Notably, these mutations were detected at distinct time points during the course of treatment by means of NGS to monitor the kinetics of the infection. Moreover, we conducted phenotypic resistance testing to further elucidate the properties of the infrequent A605V mutation.

CASE REPORT

We present a 61-year-old male with chronic lymphocytic leukaemia who underwent a nonmyeloablative allogeneic cord blood stem cell transplantation (allo-HSCT). Before transplantation, the patient was HSV-1 seropositive. ValACV (500 mg, 2 times a day orally) was prescribed as varicella zoster and HSV prophylaxis 1 week before allo-HSCT. At day 22, a swab of oral ulcerations tested positive for HSV-1 by real-time polymerase chain reaction (PCR), and ValACV treatment (500 mg, 3 times a day orally) was initiated and after 1 day modified to intravenous ACV (5 mg/kg, thrice daily) (Figure 1). Due to persistent herpetic stomatitis, intravenous ACV was increased to 10 mg/kg thrice daily on day 34. This adjustment was followed by a transition to intravenous FOS on day 36 due to the identification of a mutation in the HSV-1 TK gene, specifically a premature stop codon at amino acid position 281 (R281STOP), which coincided with a concurrent reactivation of cytomegalovirus (CMV). The R281STOP mutation is associated with reduced susceptibility to ACV, brivudine, and famciclovir in vitro [2]. Despite the administration of FOS, there was no discernible improvement in the herpetic lesions. Consequently, the treatment was reverted to ValACV. Subsequent genotypic resistance analysis of a patient sample collected at day 49 unveiled a rare mutation in the DNA pol gene (A605V). This mutation is known to confer resistance to both ACV (ACVr) and FOS (FOSr) [2]. Following this discovery, treatment with ACV was switched to intravenous CDV administration. Valganciclovir was added to the antiviral regimen due to persistent CMV reactivation. After 3 weeks on CDV, the herpetic lesions improved and CDV was switched to oral famciclovir to prevent varicella-zoster virus (VZV) and HSV reactivation. As there were no other oral alternatives for prophylaxis, famciclovir was prescribed despite the possibility of cross-resistance of (latent) HSV-1 to famciclovir based on the previously detected R281STOP mutation. On day 104, the oral herpetic lesions recurred, and repeated genotypic analysis revealed re-emergence of the R281STOP mutation, but not the A605V mutation. Unfortunately, the patient died afterwards from complications of graft-vs-host disease.

Figure 1. Overview of the clinical course and treatment of multidrug-resistant herpes simplex virus 1 reactivation in a post–allogenic hematopoietic stem cell transplant patient. The patient was treated for HSV-1 stomatitis as follows: day 22 after transplantation, positive HSV-1 real-time PCR, treatment with valaciclovir initiated; day 34, switch to aciclovir due to disease progression; day 36, switch to foscarnet due to concurrent CMV reactivation and the finding of an acyclovir-associated resistance mutation (R281STOP) in the TK gene; day 49, detection of mutation in the DNA pol gene (A605V) associated with resistance to aciclovir and foscarnet, subsequent switch to cidofovir at day 59 until resolution of HSV-1 stomatitis; day 104, recurrence of herpetic lesions, R281STOP mutation detected, A605V mutation not detected. Abbreviations: CMV, cytomegalovirus; DNA pol, DNA polymerase; HSV, herpes simplex virus; PCR, polymerase chain reaction; qPCR, quantitative PCR; TK, thymidine kinase.

RESULTS

Based on the dynamic occurrence of the 2 drug resistance mutations during treatment, we hypothesized that multiple drug-resistant HSV-1 variants might be present in the patient. To validate this hypothesis, we performed NGS on patient samples obtained at days 22, 28, 34, 64, and 104 post-allo-HSCT. The regions of the viral TK and pol genes that include the R281STOP and A605V mutations were PCR-amplified and subjected to Illumina sequencing. Upon aligning the sequences to the reference HSV-1 strain (GenBank accession number: JQ673480), both mutations were identified. Notably, at day 22, post-transplantation sequences derived from both TK_R281STOP- and DNA pol_A605V-containing viruses were simultaneously present in, respectively, 39.94% and 0.13% of the sequences (Table 1). At that time point, antiviral therapy had not yet been initiated. Under antiviral pressure in vivo, the ratio of HSV-1 strains carrying these mutations changed dramatically over time.

Table 1. Summary of the Results Obtained From Breseq

Days After Transplantation	TK_R281STOP, %	Total Reads	DNA Pol_A605V, %	Total Reads	
22	39.94	145 163	0.13	373 554	
28	98.77	257 124	0.83	396 946	
34	94.76	358 015	0.66	372 147	
64	1.09	282 560	31.21	632 980	
104	99.75	400 428	0.30	413 501	
The mutation percentages (TK_R281STOP and DNA_pol_A605 V) were calculated by dividing the total amount of mapped reads containing the specific alternative base at the specific position by the total amount of reads mapped to that position (Total Reads). TK and DNA pol fragments were amplified in different runs causing differences in total number of reads (Supplementary Data).

In vitro phenotypic characterization was performed to validate the phenotypic antiviral resistance profile of our patient-derived HSV-1 isolate harboring the DNA pol_A605V mutation. Our analysis confirmed resistance of the HSV-1 DNA pol_A605V isolate to FOS with concentrations exceeding the known effective threshold of ∼100 µg/mL (Supplementary Figure 1a). At higher concentrations (600–1200 µg/mL), FOS was able to inhibit virus replication, thereby abrogating virus-induced cell death. We also investigated resistance of the HSV-1 DNA pol_A605V variant to ACV (Supplementary Figure 1b). Our results indicate that the clinical HSV-1 DNA pol_A605V isolate is resistant to ACV, with an ACV IC50 of 4.54 µg/mL. This finding aligns with the established definition of ACV resistance, where IC50 values >2 µg/mL are generally considered a cutoff for ACV resistance [9]. The ACVr and FOSr phenotypes of the DNA pol_A605V-harboring isolate corresponded with clinical failure on ACV and FOS in our patient.

DISCUSSION

Here, we report the first clinical case of an immunosuppressed patient with HSV-1 reactivation after allo-HSCT with 2 concurrent antiviral resistance mutations (TK_R281STOP and DNA pol_A605V). Further characterization using NGS revealed that these mutations were presented with dynamic ratios during antiviral treatment. This emphasizes that HSV-1 strains carrying resistance mutations can persist in latent HSV-1 and reappear under antiviral pressure.

The rare A605V mutation in the DNA pol gene has been reported occasionally [4, 10–12]. The mutation is associated with in vitro resistance to both ACV and FOS [2], which corresponds with the in vivo clinical failure on ACV and FOS observed in our patient. During follow-up, the A605 V mutation became undetectable by Sanger sequencing of the DNA pol gene in the absence of ACV and FOS, whereas the premature stopcodon mutation on position 281 in the TK gene re-appeared on ACV treatment. NGS detected the presence of both the DNA pol_A605V and TK_R281STOP mutations before antiviral treatment and indicates a mixed infection with the 2 mutations throughout the post-allo-HSCT period. Previous research has characterized HSV-1 strains with mutations in the essential DNA pol gene as having attenuated growth. TK activity is not essential for viral replication in replicating cells, such as cells used in standard cell culture [13], but is essential for viral replication in nonreplicating cells. As both the DNA pol_A605V variant and the TK_R281STOP variant retain replicative capacity [14], albeit at a reduced level, the possibility exists that both mutants established latency in nonreplicating cells such as neurons, which may explain the existence of both mutants before antiviral pressure. Different viral growth capacity of the HSV-1 resistance mutation–carrying strains may have contributed to the dynamic ratios of the 2 mutations, with the DNA pol_A605V mutant possibly exhibiting lower replicating capacity than the TK_R281STOP mutant. Thus, the 2 mutants showed compatible cooccurrence on antiviral treatment.

A diagnostic swab from the oral mucosa Is not necessarily representative of all variants that are present in a swarm of viruses in an immunocompromised person with extensive lesions.

Sanger sequencing could have missed the variant with the R281STOP mutation obtained while the patient was receiving FOS. It can also not be excluded that HSV strains may differ from each other between the anatomical locations within the same patient, though the latter seems of minor importance as clinically significant lesions only existed in the oral mucosa.

Our findings highlight that multiple HSV-1 strains carrying different resistance-associated mutations can persist in a patient. The utilization of NGS genotyping on clinical samples is a potent method for detection of low-abundant strains carrying drug resistance mutations and facilitates swift adjustments to effective treatment strategies.

Supplementary Material

ofae250_Supplementary_Data

Acknowledgments

We thank Anita Schürch for the valuable bioinformatic input. We acknowledge the Utrecht Sequencing Facility (USEQ) for providing sequencing service and data.

Patient consent. The authors obtained written informed consent from the patient to publish individual patient data.

Ethical approval. The work presented conforms to the local currently applied standards as stated by the local ethical committee. In accordance with The Medical Research Ethics Committee (METC) Utrecht, the study was not subjected to the Medical Research Involving Human Subjects Act (WMO), and, therefore, an approval under the WMO is not required from the institutional review board of University Medical Center Utrecht.

Financial support. USEQ is subsidized by the University Medical Center Utrecht and The Netherlands X-omics Initiative (NWO project 184.034.019).

Supplementary Data

Supplementary materials are available at Open Forum Infectious Diseases online. Consisting of data provided by the authors to benefit the reader, the posted materials are not copyedited and are the sole responsibility of the authors, so questions or comments should be addressed to the corresponding author.
==== Refs
References

1 Young  J-AH, Weisdorf  DJ. 312 - Infections in recipients of hematopoietic stem cell transplants. In: Bennett  JE  Dolin  R and Blaser  MJ, eds. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. 8th ed. W.B. Saunders; 2015:3425–39.e5.
2 Sauerbrei  A, Bohn-Wippert  K, Kaspar  M, Krumbholz  A, Karrasch  M, Zell  R. Database on natural polymorphisms and resistance-related non-synonymous mutations in thymidine kinase and DNA polymerase genes of herpes simplex virus types 1 and 2. J Antimicrob Chemother  2016; 71 :6–16.26433780
3 Schubert  A, Gentner  E, Bohn  K, Schwarz  M, Mertens  T, Sauerbrei  A. Single nucleotide polymorphisms of thymidine kinase and DNA polymerase genes in clinical herpes simplex virus type 1 isolates associated with different resistance phenotypes. Antiviral Res  2014; 107 :16–22.24747042
4 Piret  J, Boivin  G. Resistance of herpes simplex viruses to nucleoside analogues: mechanisms, prevalence, and management. Antimicrob Agents Chemother  2011; 55 :459–72.21078929
5 Frobert  E, Ooka  T, Cortay  JC, Lina  B, Thouvenot  D, Morfin  F. Herpes simplex virus thymidine kinase mutations associated with resistance to acyclovir: a site-directed mutagenesis study. Antimicrob Agents Chemother  2005; 49 :1055–9.15728902
6 Zarrouk  K, Piret  J, Boivin  G. Herpesvirus DNA polymerases: structures, functions and inhibitors. Virus Res  2017; 234 :177–92.28153606
7 Morfin  F, Thouvenot  D. Herpes simplex virus resistance to antiviral drugs. J Clin Virol  2003; 26 :29–37.12589832
8 Mercier-Darty  M, Boutolleau  D, Rodriguez  C, Burrel  S. Added value of ultra-deep sequencing (UDS) approach for detection of genotypic antiviral resistance of herpes simplex virus (HSV). Antiviral Res  2019; 168 :128–33.31158412
9 Schalkwijk  HH, Snoeck  R, Andrei  G. Acyclovir resistance in herpes simplex viruses: prevalence and therapeutic alternatives. Biochem Pharmacol  2022; 206 :115322.36309081
10 Saijo  M, Suzutani  T, Morikawa  S, Kurane  I. Genotypic characterization of the DNA polymerase and sensitivity to antiviral compounds of foscarnet-resistant herpes simplex virus type 1 (HSV-1) derived from a foscarnet-sensitive HSV-1 strain. Antimicrob Agents Chemother  2005; 49 :606–11.15673740
11 Frobert  E, Burrel  S, Ducastelle-Lepretre  S, et al  Resistance of herpes simplex viruses to acyclovir: an update from a ten-year survey in France. Antiviral Res  2014; 111 :36–41.25218782
12 Kakiuchi  S, Tsuji  M, Nishimura  H, et al  Association of the emergence of acyclovir-resistant herpes simplex virus type 1 with prognosis in hematopoietic stem cell transplantation patients. J Infect Dis  2017; 215 :865–73.28453848
13 Field  HJ, Wildy  P. The pathogenicity of thymidine kinase-deficient mutants of herpes simplex virus in mice. J Hyg (Lond)  1978; 81 :267–77.212476
14 Gus’kova  AA, Skoblov  MY, Korovina  AN, et al  Antiherpetic properties of acyclovir 5'-hydrogenphosphonate and the mutation analysis of herpes virus resistant strains. Chem Biol Drug Des  2009; 74 :382–9.19691467
