PMID- 10659995
OWN - NLM
STAT- MEDLINE
DCOM- 20000313
LR  - 20190921
IS  - 0898-6568 (Print)
IS  - 0898-6568 (Linking)
VI  - 11
IP  - 12
DP  - 1999 Dec
TI  - Co-expression of human Kir3 subunits can yield channels with different functional
      properties.
PG  - 871-83
AB  - To date, no comprehensive study has been done on all combinations of the human
      homologues of the Kir3.0 channel family, and the human homologue of Kir3.3 has
      not yet been identified. To obtain support for the contention that most of the
      functional data on non-human Kir3.0 channels can be extrapolated to human
      channels, we have cloned the human homologues of the Kir3.0 family, including the
      yet unidentified human Kir3.3, and the human Kir4.1. The expression pattern of
      these channels in various human brain areas and peripheral tissues, analysed by
      Northern blot analysis, allows for the existence of various homomeric and
      heteromeric forms of human Kir3.0 channels. Expression studies of all possible
      combinations in Xenopus oocytes indicated that in homomeric Kir3.2c and
      heteromeric Kir3.1/3.2c channels mediate, in our studies, inward currents with
      largest amplitude of any other Kir3.0 channel combinations, followed by
      heteromeric Kir3.1/3.4 and homomeric Kir4.1 channels. Channel combinations which 
      include Kir3.3 are detrimental to the formation of functional channels. The
      co-expression experiments with different Kir channel subunits indicate the
      selective formation of certain channel combinations, suggesting that channel
      specificity is not solely dependent on spatial and temporal regulation of Kir
      subunit expression.
FAU - Schoots, O
AU  - Schoots O
AD  - Laboratory for Molecular Neurobiology, Centre for Addiction and Mental Health,
      Clarke Div., Toronto, Ontario, Canada.
FAU - Wilson, J M
AU  - Wilson JM
FAU - Ethier, N
AU  - Ethier N
FAU - Bigras, E
AU  - Bigras E
FAU - Hebert, T E
AU  - Hebert TE
FAU - Van Tol, H H
AU  - Van Tol HH
LA  - eng
SI  - GENBANK/U50964
SI  - GENBANK/U52152
SI  - GENBANK/U52153
SI  - GENBANK/U52154
SI  - GENBANK/U52155
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Signal
JT  - Cellular signalling
JID - 8904683
RN  - 0 (DNA, Complementary)
RN  - 0 (G Protein-Coupled Inwardly-Rectifying Potassium Channels)
RN  - 0 (KCNJ9 protein, human)
RN  - 0 (Kcnj9 protein, mouse)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Potassium Channels)
RN  - 0 (Potassium Channels, Inwardly Rectifying)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - RWP5GA015D (Potassium)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Brain/metabolism
MH  - Cerebellum/metabolism
MH  - DNA, Complementary/genetics
MH  - Female
MH  - G Protein-Coupled Inwardly-Rectifying Potassium Channels
MH  - GTP-Binding Proteins/physiology
MH  - Humans
MH  - Ion Channel Gating
MH  - Ion Transport
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/chemistry/metabolism
MH  - Oocytes
MH  - Organ Specificity
MH  - Potassium/*metabolism
MH  - Potassium Channels/chemistry/*metabolism
MH  - *Potassium Channels, Inwardly Rectifying
MH  - Recombinant Fusion Proteins/metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Species Specificity
MH  - Xenopus laevis
EDAT- 2000/02/05 09:00
MHDA- 2000/03/18 09:00
CRDT- 2000/02/05 09:00
PHST- 2000/02/05 09:00 [pubmed]
PHST- 2000/03/18 09:00 [medline]
PHST- 2000/02/05 09:00 [entrez]
AID - S0898-6568(99)00059-5 [pii]
AID - 10.1016/s0898-6568(99)00059-5 [doi]
PST - ppublish
SO  - Cell Signal. 1999 Dec;11(12):871-83. doi: 10.1016/s0898-6568(99)00059-5.