PMID- 10659855
OWN - NLM
STAT- MEDLINE
DCOM- 20000211
LR  - 20131121
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 403
IP  - 6767
DP  - 2000 Jan 20
TI  - The joining of ribosomal subunits in eukaryotes requires eIF5B.
PG  - 332-5
AB  - Initiation of eukaryotic protein synthesis begins with the ribosome separated
      into its 40S and 60S subunits. The 40S subunit first binds eukaryotic initiation 
      factor (eIF) 3 and an eIF2-GTP-initiator transfer RNA ternary complex. The
      resulting complex requires eIF1, eIF1A, eIF4A, eIF4B and eIF4F to bind to a
      messenger RNA and to scan to the initiation codon. eIF5 stimulates hydrolysis of 
      eIF2-bound GTP and eIF2 is released from the 48S complex formed at the initiation
      codon before it is joined by a 60S subunit to form an active 80S ribosome. Here
      we show that hydrolysis of eIF2-bound GTP induced by eIF5 in 48S complexes is
      necessary but not sufficient for the subunits to join. A second factor termed
      eIF5B (relative molecular mass 175,000) is essential for this process. It is a
      homologue of the prokaryotic initiation factor IF2 (re and, like it, mediates
      joining of subunits and has a ribosome-dependent GTPase activity that is
      essential for its function.
FAU - Pestova, T V
AU  - Pestova TV
AD  - Department of Microbiology and Immunology, State University of New York Health
      Science Center at Brooklyn, 11203, USA. tpestova@netmail.hscbklyn.edu
FAU - Lomakin, I B
AU  - Lomakin IB
FAU - Lee, J H
AU  - Lee JH
FAU - Choi, S K
AU  - Choi SK
FAU - Dever, T E
AU  - Dever TE
FAU - Hellen, C U
AU  - Hellen CU
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (Codon, Initiator)
RN  - 0 (Eukaryotic Initiation Factor-1)
RN  - 0 (Eukaryotic Initiation Factor-2)
RN  - 0 (Eukaryotic Initiation Factor-3)
RN  - 0 (Eukaryotic Initiation Factor-5)
RN  - 0 (Peptide Initiation Factors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (eukaryotic peptide initiation factor-1A)
RN  - 34273-04-6 (Guanylyl Imidodiphosphate)
RN  - 4A6ZS6Q2CL (Puromycin)
RN  - 6042-08-6 (methionylpuromycin)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Catalysis
MH  - Codon, Initiator
MH  - Eukaryotic Initiation Factor-1/metabolism
MH  - Eukaryotic Initiation Factor-2/metabolism
MH  - Eukaryotic Initiation Factor-3
MH  - Eukaryotic Initiation Factor-5
MH  - GTP Phosphohydrolases/metabolism
MH  - Guanosine Triphosphate/metabolism
MH  - Guanylyl Imidodiphosphate/metabolism
MH  - Humans
MH  - Hydrolysis
MH  - Molecular Sequence Data
MH  - *Peptide Chain Initiation, Translational
MH  - Peptide Initiation Factors/*metabolism
MH  - Puromycin/*analogs & derivatives/biosynthesis
MH  - RNA, Messenger/metabolism
MH  - Recombinant Proteins/metabolism
MH  - Ribosomes/*metabolism
EDAT- 2000/02/05 09:00
MHDA- 2000/02/19 09:00
CRDT- 2000/02/05 09:00
PHST- 2000/02/05 09:00 [pubmed]
PHST- 2000/02/19 09:00 [medline]
PHST- 2000/02/05 09:00 [entrez]
AID - 10.1038/35002118 [doi]
PST - ppublish
SO  - Nature. 2000 Jan 20;403(6767):332-5. doi: 10.1038/35002118.