PMID- 10657629
OWN - NLM
STAT- MEDLINE
DCOM- 20000309
LR  - 20190515
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 164
IP  - 4
DP  - 2000 Feb 15
TI  - Cloning and characterization of IL-10-related T cell-derived inducible factor
      (IL-TIF), a novel cytokine structurally related to IL-10 and inducible by IL-9.
PG  - 1814-9
AB  - IL-9 is a Th2 cytokine active on various cell types such as T and B lymphocytes, 
      mast cells, and eosinophils, and potentially involved in allergy and asthma. To
      understand better the molecular mechanisms underlying the activity of this
      cytokine, we used a cDNA subtraction method to identify genes specifically
      induced by IL-9 in mouse T cells. One of the IL-9-regulated genes isolated by
      this approach turned out to encode a 180-amino acid long protein, including a
      potential signal peptide, and showing 22% amino acid identity with IL-10. This
      protein, designated IL-10-related T cell-derived inducible factor (IL-TIF), is
      induced by IL-9 in thymic lymphomas, T cells, and mast cells, and by lectins in
      freshly isolated splenocytes. Experiments concerning the mechanism regulating
      IL-TIF expression in T cells indicate that IL-9 induction is rapid (within 1 h), 
      does not require protein synthesis, and depends on the activation of the Janus
      kinase (JAK)-STAT pathway. In vivo, constitutive expression of IL-TIF was
      detected by RT-PCR in thymus and brain, suggesting that the role of this new
      factor is not restricted to the immune system. Transfection of HEK293 cells with 
      the IL-TIF cDNA resulted in the production of a glycosylated protein of about 25 
      kDa that was found to induce STAT activation in mesangial and neuronal cell
      lines. Further studies will have to address the possibility that some of the IL-9
      activities may be mediated by IL-TIF.
FAU - Dumoutier, L
AU  - Dumoutier L
AD  - Ludwig Institute for Cancer Research, Brussels, Belgium.
FAU - Louahed, J
AU  - Louahed J
FAU - Renauld, J C
AU  - Renauld JC
LA  - eng
SI  - GENBANK/AJ249491
SI  - GENBANK/AJ249492
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Cytokines)
RN  - 0 (IL-10-related T cell-derived inducible factor, IL-TIF)
RN  - 0 (Interleukin-9)
RN  - 0 (RNA, Messenger)
RN  - 130068-27-8 (Interleukin-10)
SB  - AIM
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Cytokines/biosynthesis/*chemistry/*genetics
MH  - Gene Expression Regulation/immunology
MH  - Humans
MH  - Interleukin-10/*chemistry
MH  - Interleukin-9/*physiology
MH  - Lymphoma, T-Cell/genetics/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - RNA, Messenger/biosynthesis
MH  - *Sequence Homology, Amino Acid
MH  - T-Lymphocytes/*metabolism
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 2000/02/05 09:00
MHDA- 2000/03/11 09:00
CRDT- 2000/02/05 09:00
PHST- 2000/02/05 09:00 [pubmed]
PHST- 2000/03/11 09:00 [medline]
PHST- 2000/02/05 09:00 [entrez]
AID - ji_v164n4p1814 [pii]
AID - 10.4049/jimmunol.164.4.1814 [doi]
PST - ppublish
SO  - J Immunol. 2000 Feb 15;164(4):1814-9. doi: 10.4049/jimmunol.164.4.1814.