PMID- 10656795
OWN - NLM
STAT- MEDLINE
DCOM- 20000307
LR  - 20071115
IS  - 0022-2836 (Print)
IS  - 0022-2836 (Linking)
VI  - 295
IP  - 4
DP  - 2000 Jan 28
TI  - Post-translational modification of p53 protein in response to ionizing radiation 
      analyzed by mass spectrometry.
PG  - 853-64
AB  - The p53 tumor suppressor protein promotes cell cycle arrest or apoptosis in
      response to DNA damage and other forms of stress. p53 protein functions as a
      transcription factor by binding to specific DNA sequences and regulating the
      transcription of target genes. This activity of p53 is reported to be regulated
      by phosphorylation and acetylation occuring at various sites on the molecule.
      Here, we have used a direct and non-radioactive approach involving mass
      spectrometric analysis of p53 protein to identify sites that are covalently
      modified in vivo, either constitutively or in response to ionizing radiation.
      Following partial purification by immuno-affinity chromatography and enzymatic
      in-gel digestion, the resulting p53 peptides were analyzed by MALDI-TOF and
      nanoelectrospray mass spectrometry. Mass spectrometry analyses identified four
      sites at the N terminus that were phosphorylated in response to irradiation, a
      single constitutive phosphorylation site at serine 315 and several acetylation
      sites.
CI  - Copyright 2000 Academic Press.
FAU - Abraham, J
AU  - Abraham J
AD  - Ontario Cancer Institute/Princess Margaret Hospital and Department of Medical
      Biophysics, Toronto, Ontario, M5G 2M9, Canada.
FAU - Kelly, J
AU  - Kelly J
FAU - Thibault, P
AU  - Thibault P
FAU - Benchimol, S
AU  - Benchimol S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Mol Biol
JT  - Journal of molecular biology
JID - 2985088R
RN  - 0 (Peptide Fragments)
RN  - 0 (Protein Isoforms)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 17885-08-4 (Phosphoserine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Humans
MH  - Leukemia, Myeloid, Acute
MH  - Molecular Sequence Data
MH  - Peptide Fragments/chemistry/isolation & purification
MH  - Phosphorylation
MH  - Phosphoserine/analysis
MH  - Protein Isoforms/chemistry/metabolism/radiation effects
MH  - Protein Processing, Post-Translational/*radiation effects
MH  - Radiation, Ionizing
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Tumor Cells, Cultured
MH  - Tumor Suppressor Protein p53/*chemistry/*metabolism/radiation effects
EDAT- 2000/02/05 09:00
MHDA- 2000/03/11 09:00
CRDT- 2000/02/05 09:00
PHST- 2000/02/05 09:00 [pubmed]
PHST- 2000/03/11 09:00 [medline]
PHST- 2000/02/05 09:00 [entrez]
AID - 10.1006/jmbi.1999.3415 [doi]
AID - S0022-2836(99)93415-0 [pii]
PST - ppublish
SO  - J Mol Biol. 2000 Jan 28;295(4):853-64. doi: 10.1006/jmbi.1999.3415.