PMID- 10656792
OWN - NLM
STAT- MEDLINE
DCOM- 20000307
LR  - 20171116
IS  - 0022-2836 (Print)
IS  - 0022-2836 (Linking)
VI  - 295
IP  - 4
DP  - 2000 Jan 28
TI  - DNA polymerase switching: II. Replication factor C abrogates primer synthesis by 
      DNA polymerase alpha at a critical length.
PG  - 803-14
AB  - A crucial event in DNA replication is the polymerase switch from the synthesis of
      a short RNA/DNA primer by DNA polymerase alpha/primase to the pro?cessive
      elongation by DNA polymerase delta. In order to shed light on the role of
      replication factor C (RF-C) in this process, the effects of RF-C on DNA
      polymerase alpha were investigated. We show that RF-C stalls DNA polymerase alpha
      after synthesis of approximately 30 nucleotides, while not inhibiting the
      polymerase activity per se. This suggested that RF-C and the length of the primer
      may be two important factors contributing to the polymerase switch. Furthermore
      the DNA binding properties of RF-C were tested. Band shift experiments indicated 
      that RF-C has a preference for 5' recessed ends and double-stranded DNA over 3'
      ends. Finally PCNA can be loaded onto a DNA template carrying a RNA primer,
      suggesting that a DNA moiety is not necessarily required for the loading of the
      clamp. Thus we propose a model where RF-C, upon binding to the RNA/DNA primer,
      influences primer synthesis and sets the conditions for a polymerase switch after
      recruiting PCNA to DNA.
CI  - Copyright 2000 Academic Press.
FAU - Mossi, R
AU  - Mossi R
AD  - Department of Veterinary Biochemistry, University of Zurich-Irchel,
      Winterthurerstrasse 190, Zurich, CH-8057, Switzerland.
FAU - Keller, R C
AU  - Keller RC
FAU - Ferrari, E
AU  - Ferrari E
FAU - Hubscher, U
AU  - Hubscher U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Mol Biol
JT  - Journal of molecular biology
JID - 2985088R
RN  - 0 (BCL2-related protein A1)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (MATA1 protein, S cerevisiae)
RN  - 0 (Minor Histocompatibility Antigens)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.7.- (DNA Polymerase I)
RN  - EC 2.7.7.- (DNA Primase)
RN  - EC 3.6.4.- (Replication Protein C)
SB  - IM
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA/*biosynthesis/*chemistry
MH  - DNA Polymerase I/*metabolism
MH  - DNA Primase/metabolism
MH  - *DNA Replication
MH  - DNA-Binding Proteins/*metabolism
MH  - Escherichia coli
MH  - *Homeodomain Proteins
MH  - Humans
MH  - Minor Histocompatibility Antigens
MH  - Molecular Sequence Data
MH  - Oligodeoxyribonucleotides
MH  - Proliferating Cell Nuclear Antigen/metabolism
MH  - *Proto-Oncogene Proteins c-bcl-2
MH  - Recombinant Proteins/metabolism
MH  - Replication Protein C
MH  - *Repressor Proteins
MH  - *Saccharomyces cerevisiae Proteins
MH  - Templates, Genetic
EDAT- 2000/02/05 09:00
MHDA- 2000/03/11 09:00
CRDT- 2000/02/05 09:00
PHST- 2000/02/05 09:00 [pubmed]
PHST- 2000/03/11 09:00 [medline]
PHST- 2000/02/05 09:00 [entrez]
AID - 10.1006/jmbi.1999.3395 [doi]
AID - S0022-2836(99)93395-8 [pii]
PST - ppublish
SO  - J Mol Biol. 2000 Jan 28;295(4):803-14. doi: 10.1006/jmbi.1999.3395.