PMID- 10656791
OWN - NLM
STAT- MEDLINE
DCOM- 20000307
LR  - 20171116
IS  - 0022-2836 (Print)
IS  - 0022-2836 (Linking)
VI  - 295
IP  - 4
DP  - 2000 Jan 28
TI  - DNA polymerase switching: I. Replication factor C displaces DNA polymerase alpha 
      prior to PCNA loading.
PG  - 791-801
AB  - An important not yet fully understood event in DNA replication is the DNA
      polymerase (pol) switch from pol alpha to pol delta. Indirect evidence suggested 
      that the clamp loader replication factor C (RF-C) plays an important role, since 
      a replication competent protein complex containing pol alpha, pol delta and RF-C 
      could perform pol switching in the presence of proliferating cell nuclear antigen
      (PCNA). By using purified pol alpha/primase, pol delta, RF-C, PCNA and RP-A we
      show that: (i) RF-C can inhibit pol alpha in the presence of ATP prior to PCNA
      loading, (ii) RF-C decreases the affinity of pol alpha for the 3'OH primer ends, 
      (iii) the inhibition of pol alpha by RF-C is released upon PCNA loading, (iv) ATP
      hydrolysis is required for PCNA loading and subsequent release of inhibition of
      pol alpha, (v) under these conditions a switching from pol alpha/primase to pol
      delta is evident. Thus, RF-C appears to be critical for the pol alpha to pol
      delta switching. Based on these results, a model is proposed in which RF-C
      induces the pol switching by sequestering the 3'-OH end from pol alpha and
      subsequently recruiting PCNA to DNA.
CI  - Copyright 2000 Academic Press.
FAU - Maga, G
AU  - Maga G
AD  - Istituto di Genetica Biochimica ed Evoluzionistica IGBE-CNR, Pavia, I-27100,
      Italy.
FAU - Stucki, M
AU  - Stucki M
FAU - Spadari, S
AU  - Spadari S
FAU - Hubscher, U
AU  - Hubscher U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Mol Biol
JT  - Journal of molecular biology
JID - 2985088R
RN  - 0 (BCL2-related protein A1)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (MATA1 protein, S cerevisiae)
RN  - 0 (Minor Histocompatibility Antigens)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Repressor Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 2.7.7.- (DNA Polymerase I)
RN  - EC 2.7.7.- (DNA Polymerase III)
RN  - EC 2.7.7.- (DNA Primase)
RN  - EC 3.6.4.- (Replication Protein C)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Cattle
MH  - Cytosol/enzymology
MH  - DNA Polymerase I/*metabolism
MH  - DNA Polymerase III/*metabolism
MH  - DNA Primase/metabolism
MH  - *DNA Replication
MH  - DNA-Binding Proteins/*metabolism
MH  - HeLa Cells
MH  - *Homeodomain Proteins
MH  - Humans
MH  - Kinetics
MH  - Minor Histocompatibility Antigens
MH  - Proliferating Cell Nuclear Antigen/*metabolism
MH  - *Proto-Oncogene Proteins c-bcl-2
MH  - Replication Protein C
MH  - *Repressor Proteins
MH  - *Saccharomyces cerevisiae Proteins
MH  - Simian virus 40/genetics/physiology
MH  - Substrate Specificity
MH  - Thymus Gland/enzymology
MH  - Virus Replication
EDAT- 2000/02/05 09:00
MHDA- 2000/03/11 09:00
CRDT- 2000/02/05 09:00
PHST- 2000/02/05 09:00 [pubmed]
PHST- 2000/03/11 09:00 [medline]
PHST- 2000/02/05 09:00 [entrez]
AID - 10.1006/jmbi.1999.3394 [doi]
AID - S0022-2836(99)93394-6 [pii]
PST - ppublish
SO  - J Mol Biol. 2000 Jan 28;295(4):791-801. doi: 10.1006/jmbi.1999.3394.