PMID- 10656772
OWN - NLM
STAT- MEDLINE
DCOM- 20000309
LR  - 20220818
IS  - 0012-1606 (Print)
IS  - 0012-1606 (Linking)
VI  - 218
IP  - 2
DP  - 2000 Feb 15
TI  - Membrane fusion proteins are required for oskar mRNA localization in the 
      Drosophila egg chamber.
PG  - 314-25
AB  - We used a genetic screen in Drosophila to identify mutations which disrupt the 
      localization of oskar mRNA during oogenesis. Based on the hypothesis that some 
      cytoskeletal components which are required during the mitotic divisions will also 
      be required for oskar mRNA localization during oogenesis, we designed the 
      following genetic screen. We screened for P-element insertions in genes which 
      slow down the blastoderm mitotic divisions. A secondary genetic screen was to 
      generate female germ-line clones of these potential cell division cycle genes and 
      to identify those which cause the mislocalization of oskar mRNA. We identified 
      mutations in ter94 which disrupt the localization of oskar mRNA to the posterior 
      pole of the oocyte. Ter94 is a member of the CDC48p/VCP subfamily of AAA proteins 
      which are involved in homotypic fusion of the endoplasmic reticulum during 
      mitosis. Consistent with the function of the yeast ortholog, ter94-mutant egg 
      chambers are defective in the assembly of the endoplasmic reticulum. We tested 
      whether other membrane biosynthesis genes are required for localizing oskar mRNA 
      during oogenesis. We found that ovaries that are mutant for syntaxin-1a, rop, and 
      synaptotagmin are also defective in oskar mRNA localization during oogenesis. We 
      suggest a pathway for the role of membrane assembly proteins on oskar mRNA 
      localization.
CI  - Copyright 2000 Academic Press.
FAU - Ruden, D M
AU  - Ruden DM
AD  - Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas 
      66045, USA. ruden@eagle.cc.ukans.edu
FAU - Sollars, V
AU  - Sollars V
FAU - Wang, X
AU  - Wang X
FAU - Mori, D
AU  - Mori D
FAU - Alterman, M
AU  - Alterman M
FAU - Lu, X
AU  - Lu X
LA  - eng
GR  - R01 AA012276/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (Drosophila Proteins)
RN  - 0 (Insect Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (osk protein, Drosophila)
SB  - IM
MH  - Animals
MH  - Drosophila/*genetics
MH  - *Drosophila Proteins
MH  - Endoplasmic Reticulum/metabolism
MH  - Female
MH  - Insect Proteins/*genetics
MH  - *Membrane Fusion
MH  - Mutation
MH  - Oogenesis
MH  - Phenotype
MH  - RNA, Messenger/genetics/*metabolism
EDAT- 2000/02/05 09:00
MHDA- 2000/03/11 09:00
CRDT- 2000/02/05 09:00
PHST- 2000/02/05 09:00 [pubmed]
PHST- 2000/03/11 09:00 [medline]
PHST- 2000/02/05 09:00 [entrez]
AID - S0012-1606(99)99583-0 [pii]
AID - 10.1006/dbio.1999.9583 [doi]
PST - ppublish
SO  - Dev Biol. 2000 Feb 15;218(2):314-25. doi: 10.1006/dbio.1999.9583.