PMID- 10656688
OWN - NLM
STAT- MEDLINE
DCOM- 20000210
LR  - 20131121
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 19
IP  - 3
DP  - 2000 Jan 20
TI  - Cell cycle regulated expression and phosphorylation of hpttg proto-oncogene
      product.
PG  - 403-9
AB  - We recently isolated a cDNA for hpttg, the human homolog of rat pituitary tumor
      transforming gene. Now we have analysed the expression of hpttg as a function of 
      cell proliferation. hPTTG protein level is up-regulated in rapidly proliferating 
      cells, is down-regulated in response to serum starvation or cell confluence, and 
      is regulated in a cell cycle-dependent manner, peaking in mitosis. In addition,
      we show that hPTTG is phosphorylated during mitosis. Immunodepletion and in vitro
      phosphorylation experiments, together with the use of a specific inhibitor,
      indicate that Cdc2 is the kinase that phosphorylates hPTTG. These results suggest
      that hpttg is induced by, and may have a role in, regulatory pathways involved in
      the control of cell proliferation.
FAU - Ramos-Morales, F
AU  - Ramos-Morales F
AD  - Departamento de Microbiologia, Facultad de Biologia, Universidad de Seville,
      Spain.
FAU - Dominguez, A
AU  - Dominguez A
FAU - Romero, F
AU  - Romero F
FAU - Luna, R
AU  - Luna R
FAU - Multon, M C
AU  - Multon MC
FAU - Pintor-Toro, J A
AU  - Pintor-Toro JA
FAU - Tortolero, M
AU  - Tortolero M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Oncogene Proteins)
RN  - 0 (Securin)
RN  - EC 2.7.11.22 (CDC2 Protein Kinase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - CDC2 Protein Kinase/physiology
MH  - COS Cells
MH  - Cell Cycle
MH  - Cell Division
MH  - HeLa Cells
MH  - Humans
MH  - Mitosis
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/*metabolism
MH  - Oncogene Proteins/*metabolism
MH  - Phosphorylation
MH  - Securin
MH  - src Homology Domains
EDAT- 2000/02/03 00:00
MHDA- 2000/02/03 00:01
CRDT- 2000/02/03 00:00
PHST- 2000/02/03 00:00 [pubmed]
PHST- 2000/02/03 00:01 [medline]
PHST- 2000/02/03 00:00 [entrez]
AID - 10.1038/sj.onc.1203320 [doi]
PST - ppublish
SO  - Oncogene. 2000 Jan 20;19(3):403-9. doi: 10.1038/sj.onc.1203320.