PMID- 10656685
OWN - NLM
STAT- MEDLINE
DCOM- 20000210
LR  - 20211203
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 19
IP  - 3
DP  - 2000 Jan 20
TI  - The human non-muscle alpha-actinin protein encoded by the ACTN4 gene suppresses
      tumorigenicity of human neuroblastoma cells.
PG  - 380-6
AB  - alpha-Actinins are actin-binding proteins important in organization of the
      cytoskeleton and in cell adhesion. We have cloned and characterized a cDNA from
      human neuroblastoma cell variants which encodes the second non-muscle
      alpha-actinin isoform designated ACTN4 (actinin-4). mRNA encoded by the ACTN4
      gene, mapped to chromosome 4, is abundant in non-tumorigenic, substrate-adherent 
      human neuroblastoma cell variants but absent or only weakly expressed in
      malignant, poorly substrate-adherent neuroblasts. It is also present in many
      adherent tumor cell lines of diverse tissue origins. Cell lines typically
      co-express ACTN4 and ACTN1, a second non-muscle alpha-actinin gene. Expression is
      correlated with substrate adhesivity. Analysis of deduced amino acid sequences
      suggests that the two isoforms may differ in function and in regulation by
      calcium. Moreover, ACTN4 exhibits tumor suppressor activity. Stable clones
      containing increased levels of alpha-actinin, isolated from highly malignant
      neuroblastoma stem cells [BE(2)-C] after transfection with a full-length ACTN4
      cDNA, show decreased anchorage-independent growth ability, loss of tumorigenicity
      in nude mice, and decreased expression of the N-myc proto-oncogene.
FAU - Nikolopoulos, S N
AU  - Nikolopoulos SN
AD  - Department of Biological Sciences, Fordham University, Bronx, New York, NY 10458 
      USA.
FAU - Spengler, B A
AU  - Spengler BA
FAU - Kisselbach, K
AU  - Kisselbach K
FAU - Evans, A E
AU  - Evans AE
FAU - Biedler, J L
AU  - Biedler JL
FAU - Ross, R A
AU  - Ross RA
LA  - eng
SI  - GENBANK/U48734
PT  - Journal Article
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (MAS1 protein, human)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Proto-Oncogene Mas)
RN  - 0 (RNA, Messenger)
RN  - 11003-00-2 (Actinin)
SB  - IM
MH  - Actinin/analysis/genetics/*physiology
MH  - Animals
MH  - Base Sequence
MH  - Cell Adhesion
MH  - Cell Differentiation
MH  - Cell Line
MH  - Cell Transformation, Neoplastic
MH  - Chromosome Mapping
MH  - Cytoskeleton/chemistry
MH  - *Genes, Tumor Suppressor
MH  - Humans
MH  - Mice
MH  - Mice, Nude
MH  - Microfilament Proteins/analysis
MH  - Molecular Sequence Data
MH  - Neuroblastoma/*genetics
MH  - Proto-Oncogene Mas
MH  - RNA, Messenger/analysis
EDAT- 2000/02/03 00:00
MHDA- 2000/02/03 00:01
CRDT- 2000/02/03 00:00
PHST- 2000/02/03 00:00 [pubmed]
PHST- 2000/02/03 00:01 [medline]
PHST- 2000/02/03 00:00 [entrez]
AID - 10.1038/sj.onc.1203310 [doi]
PST - ppublish
SO  - Oncogene. 2000 Jan 20;19(3):380-6. doi: 10.1038/sj.onc.1203310.