PMID- 10656681
OWN - NLM
STAT- MEDLINE
DCOM- 20000210
LR  - 20191126
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 19
IP  - 3
DP  - 2000 Jan 20
TI  - Self-association of the SET domains of human ALL-1 and of Drosophila TRITHORAX
      and ASH1 proteins.
PG  - 351-7
AB  - The human ALL-1 gene is involved in acute leukemia through gene fusions, partial 
      tandem duplications or a specific deletion. Several sequence motifs within the
      ALL-1 protein, such as the SET domain, PHD fingers and the region with homology
      to DNA methyl transferase are shared with other proteins involved in
      transcription regulation through chromatin alterations. However, the function of 
      these motifs is still not clear. Studying ALL-1 presents an additional challenge 
      because the gene is the human homologue of Drosophila trithorax. The latter is a 
      member of the trithorax-Polycomb gene family which acts to determine the body
      pattern of Drosophila by maintaining expression or repression of the
      Antennapedia-bithorax homeotic gene complex. Here we apply yeast two hybrid
      methodology, in vivo immunoprecipitation and in vitro 'pull down' techniques to
      show self association of the SET motifs of ALL-1, TRITHORAX and ASH1 proteins
      (Drosophila ASH1 is encoded by a trithorax-group gene). Point mutations in
      evolutionary conserved residues of TRITHORAX SET, abolish the interaction.
      SET-SET interactions might act in integrating the activity of ALL-1 (TRX and
      ASH1) protein molecules, simultaneously positioned at different maintenance
      elements and directing expression of the same or different target genes.
FAU - Rozovskaia, T
AU  - Rozovskaia T
AD  - Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot,
      Israel.
FAU - Rozenblatt-Rosen, O
AU  - Rozenblatt-Rosen O
FAU - Sedkov, Y
AU  - Sedkov Y
FAU - Burakov, D
AU  - Burakov D
FAU - Yano, T
AU  - Yano T
FAU - Nakamura, T
AU  - Nakamura T
FAU - Petruck, S
AU  - Petruck S
FAU - Ben-Simchon, L
AU  - Ben-Simchon L
FAU - Croce, C M
AU  - Croce CM
FAU - Mazo, A
AU  - Mazo A
FAU - Canaani, E
AU  - Canaani E
LA  - eng
GR  - CA 50507/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (ASH1 protein, Drosophila)
RN  - 0 (Chromatin)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (KMT2A protein, human)
RN  - 0 (Transcription Factors)
RN  - 0 (Trl protein, Drosophila)
RN  - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein)
RN  - EC 2.1.1.43 (ASH1L protein, human)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
SB  - IM
MH  - Amino Acid Motifs
MH  - Amino Acid Sequence
MH  - Animals
MH  - Chromatin/chemistry
MH  - Conserved Sequence
MH  - DNA-Binding Proteins/*chemistry
MH  - Drosophila
MH  - *Drosophila Proteins
MH  - Histone-Lysine N-Methyltransferase
MH  - Humans
MH  - Molecular Sequence Data
MH  - Myeloid-Lymphoid Leukemia Protein
MH  - Point Mutation
MH  - Precipitin Tests
MH  - *Proto-Oncogenes
MH  - Transcription Factors/*chemistry
EDAT- 2000/02/03 00:00
MHDA- 2000/02/03 00:01
CRDT- 2000/02/03 00:00
PHST- 2000/02/03 00:00 [pubmed]
PHST- 2000/02/03 00:01 [medline]
PHST- 2000/02/03 00:00 [entrez]
AID - 10.1038/sj.onc.1203307 [doi]
PST - ppublish
SO  - Oncogene. 2000 Jan 20;19(3):351-7. doi: 10.1038/sj.onc.1203307.