PMID- 10656257
OWN - NLM
STAT- MEDLINE
DCOM- 20000217
LR  - 20071114
IS  - 1044-7431 (Print)
IS  - 1044-7431 (Linking)
VI  - 14
IP  - 6
DP  - 1999 Dec
TI  - Reciprocal Id expression and myelin gene regulation in Schwann cells.
PG  - 519-28
AB  - Id proteins are thought to act as dominant negative antagonists of basic
      helix-loop-helix (bHLH) transcription factors that direct differentiation in
      various cell types. We found that Schwann cells express all four Id-family genes 
      and that their transcript levels were reciprocally regulated in pairs during
      nerve maturation in vivo and cAMP-mediated differentiation in vitro. The rapid
      induction as part of the early response to axonal membranes and cytokines
      suggested that Id3 is involved in myelin gene repression. An inverse relationship
      between Id1/3 and myelin P0 expression was consistent with a role for these two
      Id proteins as inhibitors of differentiation, and Id1/3 proteins strongly
      repressed myelin gene promoter activity. Nuclear factors isolated from Schwann
      cells and intact sciatic nerves were found to bind three different HLH
      recognition sequences (E boxes) in the proximal region of the P0 promoter, and
      production of these DNA binding complexes was altered during differentiation.
      These data support the concept that Id proteins regulate myelin gene expression
      by controlling the formation of specific bHLH DNA binding complexes with
      different E-box preferences.
FAU - Thatikunta, P
AU  - Thatikunta P
AD  - Children's Hospital of Philadelphia and the Department of Neurology, University
      of Pennsylvania, 19104-4318, USA.
FAU - Qin, W
AU  - Qin W
FAU - Christy, B A
AU  - Christy BA
FAU - Tennekoon, G I
AU  - Tennekoon GI
FAU - Rutkowski, J L
AU  - Rutkowski JL
LA  - eng
GR  - NS21700/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Neurosci
JT  - Molecular and cellular neurosciences
JID - 9100095
RN  - 0 (ID1 protein, rat)
RN  - 0 (Inhibitor of Differentiation Protein 1)
RN  - 0 (Myelin P0 Protein)
RN  - 0 (Myelin Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Aging/*metabolism
MH  - Animals
MH  - Cell Differentiation
MH  - Female
MH  - *Gene Expression Regulation
MH  - Gene Expression Regulation, Developmental
MH  - Helix-Loop-Helix Motifs
MH  - Inhibitor of Differentiation Protein 1
MH  - Myelin P0 Protein/*genetics
MH  - Myelin Proteins/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Repressor Proteins
MH  - Schwann Cells/cytology/*metabolism
MH  - Sciatic Nerve/growth & development/*physiology
MH  - Transcription Factors/*genetics/*metabolism
MH  - *Transcription, Genetic
EDAT- 2000/02/03 09:00
MHDA- 2000/02/19 09:00
CRDT- 2000/02/03 09:00
PHST- 2000/02/03 09:00 [pubmed]
PHST- 2000/02/19 09:00 [medline]
PHST- 2000/02/03 09:00 [entrez]
AID - S1044-7431(99)90799-6 [pii]
AID - 10.1006/mcne.1999.0799 [doi]
PST - ppublish
SO  - Mol Cell Neurosci. 1999 Dec;14(6):519-28. doi: 10.1006/mcne.1999.0799.