PMID- 10656251
OWN - NLM
STAT- MEDLINE
DCOM- 20000217
LR  - 20071115
IS  - 1044-7431 (Print)
IS  - 1044-7431 (Linking)
VI  - 14
IP  - 6
DP  - 1999 Dec
TI  - Cloning and expression of VEMA: a novel ventral midline antigen in the rat CNS.
PG  - 428-43
AB  - A variety of molecules expressed at the midline of the developing central nervous
      system (CNS) control multiple aspects of pattern formation and axon guidance. We 
      recently identified monoclonal antibody (mAb) CARO 2 as a novel marker of the
      ventral midline in the developing rat CNS, and the corresponding antigen as a
      membrane-associated 28-kDa protein. We report here the isolation of cDNA clones
      encoding the mAb CARO 2 antigen, which we rename VEMA, for ventral midline
      antigen. The deduced amino acid sequence of VEMA contains a single transmembrane 
      domain near its N-terminus and several tyrosine-based internalization motifs.
      These structural features are consistent with the association of VEMA to
      intracellular membranes. In situ hybridization analyses demonstrate that VEMA
      mRNA is predominantly expressed at the ventral midline. The restricted
      distribution of VEMA, as well as several characteristics of its primary
      structure, suggest a role for this protein in regulating axon guidance in the
      mammalian CNS.
FAU - Runko, E
AU  - Runko E
AD  - Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 
      10461, USA.
FAU - Wideman, C
AU  - Wideman C
FAU - Kaprielian, Z
AU  - Kaprielian Z
LA  - eng
SI  - GENBANK/AF163321
GR  - R29 NS34847-01/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Neurosci
JT  - Molecular and cellular neurosciences
JID - 9100095
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (VEMA antigen)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Base Sequence
MH  - Brain/cytology/*embryology/metabolism
MH  - Cell Line
MH  - Embryo, Mammalian
MH  - *Gene Expression Regulation, Developmental
MH  - Gene Library
MH  - Humans
MH  - Membrane Proteins/chemistry/*genetics/*metabolism
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/chemistry/*genetics/*metabolism
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Spinal Cord/cytology/*embryology/metabolism
MH  - Transcription, Genetic
MH  - Transfection
EDAT- 2000/02/03 09:00
MHDA- 2000/02/19 09:00
CRDT- 2000/02/03 09:00
PHST- 2000/02/03 09:00 [pubmed]
PHST- 2000/02/19 09:00 [medline]
PHST- 2000/02/03 09:00 [entrez]
AID - S1044-7431(99)90794-7 [pii]
AID - 10.1006/mcne.1999.0794 [doi]
PST - ppublish
SO  - Mol Cell Neurosci. 1999 Dec;14(6):428-43. doi: 10.1006/mcne.1999.0794.