PMID- 10655503
OWN - NLM
STAT- MEDLINE
DCOM- 20000302
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 97
IP  - 3
DP  - 2000 Feb 1
TI  - Comparative genomic sequence analysis of the human and mouse cystic fibrosis
      transmembrane conductance regulator genes.
PG  - 1172-7
AB  - The identification of the cystic fibrosis transmembrane conductance regulator
      gene (CFTR) in 1989 represents a landmark accomplishment in human genetics. Since
      that time, there have been numerous advances in elucidating the function of the
      encoded protein and the physiological basis of cystic fibrosis. However, numerous
      areas of cystic fibrosis biology require additional investigation, some of which 
      would be facilitated by information about the long-range sequence context of the 
      CFTR gene. For example, the latter might provide clues about the sequence
      elements responsible for the temporal and spatial regulation of CFTR expression. 
      We thus sought to establish the sequence of the chromosomal segments encompassing
      the human CFTR and mouse Cftr genes, with the hope of identifying conserved
      regions of biologic interest by sequence comparison. Bacterial clone-based
      physical maps of the relevant human and mouse genomic regions were constructed,
      and minimally overlapping sets of clones were selected and sequenced, eventually 
      yielding approximately 1.6 Mb and approximately 358 kb of contiguous human and
      mouse sequence, respectively. These efforts have produced the complete sequence
      of the approximately 189-kb and approximately 152-kb segments containing the
      human CFTR and mouse Cftr genes, respectively, as well as significant amounts of 
      flanking DNA. Analyses of the resulting data provide insights about the
      organization of the CFTR/Cftr genes and potential sequence elements regulating
      their expression. Furthermore, the generated sequence reveals the precise
      architecture of genes residing near CFTR/Cftr, including one known gene
      (WNT2/Wnt2) and two previously unknown genes that immediately flank CFTR/Cftr.
FAU - Ellsworth, R E
AU  - Ellsworth RE
AD  - Genome Technology Branch, National Human Genome Research Institute, National
      Institutes of Health, Bethesda, MD 20892, USA.
FAU - Jamison, D C
AU  - Jamison DC
FAU - Touchman, J W
AU  - Touchman JW
FAU - Chissoe, S L
AU  - Chissoe SL
FAU - Braden Maduro, V V
AU  - Braden Maduro VV
FAU - Bouffard, G G
AU  - Bouffard GG
FAU - Dietrich, N L
AU  - Dietrich NL
FAU - Beckstrom-Sternberg, S M
AU  - Beckstrom-Sternberg SM
FAU - Iyer, L M
AU  - Iyer LM
FAU - Weintraub, L A
AU  - Weintraub LA
FAU - Cotton, M
AU  - Cotton M
FAU - Courtney, L
AU  - Courtney L
FAU - Edwards, J
AU  - Edwards J
FAU - Maupin, R
AU  - Maupin R
FAU - Ozersky, P
AU  - Ozersky P
FAU - Rohlfing, T
AU  - Rohlfing T
FAU - Wohldmann, P
AU  - Wohldmann P
FAU - Miner, T
AU  - Miner T
FAU - Kemp, K
AU  - Kemp K
FAU - Kramer, J
AU  - Kramer J
FAU - Korf, I
AU  - Korf I
FAU - Pepin, K
AU  - Pepin K
FAU - Antonacci-Fulton, L
AU  - Antonacci-Fulton L
FAU - Fulton, R S
AU  - Fulton RS
FAU - Minx, P
AU  - Minx P
FAU - Hillier, L W
AU  - Hillier LW
FAU - Wilson, R K
AU  - Wilson RK
FAU - Waterston, R H
AU  - Waterston RH
FAU - Miller, W
AU  - Miller W
FAU - Green, E D
AU  - Green ED
LA  - eng
SI  - GENBANK/AC000061
SI  - GENBANK/AC000111
SI  - GENBANK/AC002431
SI  - GENBANK/AC002465
SI  - GENBANK/AC002529
SI  - GENBANK/AC002542
SI  - GENBANK/AC003045
SI  - GENBANK/AC003084
SI  - GENBANK/AC003987
SI  - GENBANK/AC004029
SI  - GENBANK/AC004240
SI  - GENBANK/AC004536
SI  - GENBANK/AC006326
SI  - GENBANK/AC006389
SI  - GENBANK/AC006926
SI  - GENBANK/AC007874
SI  - GENBANK/AF162137
GR  - LM05110/LM/NLM NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (CFTR protein, human)
RN  - 126880-72-6 (Cystic Fibrosis Transmembrane Conductance Regulator)
SB  - IM
MH  - Animals
MH  - Cystic Fibrosis Transmembrane Conductance Regulator/*genetics
MH  - *Genes
MH  - Humans
MH  - Mice/*genetics
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Regulatory Sequences, Nucleic Acid
MH  - Sequence Alignment
MH  - Sequence Homology, Nucleic Acid
MH  - Species Specificity
PMC - PMC15558
EDAT- 2000/02/03 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/02/03 09:00
PHST- 2000/02/03 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/02/03 09:00 [entrez]
AID - 10.1073/pnas.97.3.1172 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2000 Feb 1;97(3):1172-7. doi: 10.1073/pnas.97.3.1172.