PMID- 10655141
OWN - NLM
STAT- MEDLINE
DCOM- 20000314
LR  - 20071114
IS  - 0014-4835 (Print)
IS  - 0014-4835 (Linking)
VI  - 70
IP  - 2
DP  - 2000 Feb
TI  - A novel lens epithelium gene, LEP503, is highly conserved in different vertebrate
      species and is developmentally regulated in postnatal rat lens.
PG  - 159-68
AB  - The development of the lens is dependent on the proliferation of lens epithelial 
      cells and their differentiation into fiber cells near the lens bow/equator.
      Identification of genes specifically expressed in the lens epithelial cells and
      their functions may provide insight into molecular events that regulate the
      processes of lens epithelial cell differentiation. In this study, a novel lens
      epithelium gene product, LEP503, identified from rat by a subtractive cDNA
      cloning strategy was investigated in the genome organization, mRNA expression and
      protein localization. The genomic sequences for LEP503 isolated from rat, mouse
      and human span 1754 bp, 1694 bp and 1895 bp regions encompassing the 5'-flanking 
      region, two exons, one intron and 3'-flanking region. All exon-intron junction
      sequences conform to the GT/AG rule. Both mouse and human LEP503 genes show very 
      high identity (93% for mouse and 79% for human) to rat LEP503 gene in the exon 1 
      that contains an open reading frame coding for a protein of 61 amino acid
      residues with a leucine-rich domain. The deduced protein sequences also show high
      identity (91% between mouse and rat and 77% between human and rat). Western blot 
      shows that LEP503 is present as a specific approximately 6.9 kDa band in the
      water-insoluble-urea-soluble fraction of lens cortex where lens epithelium is
      included. Immuno-staining shows that LEP503 is localized in the epithelial cells 
      along the entire anterior surface of rat lens. Developmentally, LEP503 is
      expressed at a low level at newborn, and then the expression level increases by
      about ten-fold around postnatal day 14 and remains at this high level for about
      25 days before it drops back to the low level by postnatal day 84. These data
      suggest that the LEP503 may be an important lens epithelial cell gene involving
      the processes of epithelial cell differentiation.
CI  - Copyright 2000 Academic Press.
FAU - Wen, Y
AU  - Wen Y
AD  - Membrane Biology Laboratory, Department of Medicine, West Los Angeles VA Medical 
      Center and UCLA, Los Angeles, CA 90073, USA. ywen@ucla.edu
FAU - Sachs, G
AU  - Sachs G
FAU - Athmann, C
AU  - Athmann C
LA  - eng
GR  - DK41301/DK/NIDDK NIH HHS/United States
GR  - DK46917/DK/NIDDK NIH HHS/United States
GR  - DK53462/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Exp Eye Res
JT  - Experimental eye research
JID - 0370707
RN  - 0 (Eye Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Western
MH  - Conserved Sequence/*genetics
MH  - Epithelium
MH  - Eye Proteins/*genetics
MH  - Genome
MH  - Humans
MH  - Lens, Crystalline/cytology/growth & development/*metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - RNA, Messenger/metabolism
MH  - Rats
EDAT- 2000/02/03 09:00
MHDA- 2000/03/18 09:00
CRDT- 2000/02/03 09:00
PHST- 2000/02/03 09:00 [pubmed]
PHST- 2000/03/18 09:00 [medline]
PHST- 2000/02/03 09:00 [entrez]
AID - 10.1006/exer.1999.0770 [doi]
AID - S0014-4835(99)90770-4 [pii]
PST - ppublish
SO  - Exp Eye Res. 2000 Feb;70(2):159-68. doi: 10.1006/exer.1999.0770.