PMID- 10654943
OWN - NLM
STAT- MEDLINE
DCOM- 20000310
LR  - 20181223
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 19
IP  - 3
DP  - 2000 Feb 1
TI  - Male sterility and enhanced radiation sensitivity in TLS(-/-) mice.
PG  - 453-62
AB  - TLS (also known as FUS) is an RNA-binding protein that contributes the N-terminal
      half of fusion oncoproteins implicated in the development of human liposarcomas
      and leukemias. Here we report that male mice homozygous for an induced mutation
      in TLS are sterile with a marked increase in the number of unpaired and mispaired
      chromosomal axes in pre-meiotic spermatocytes. Nuclear extracts from TLS(-/-)
      testes lack an activity capable of promoting pairing between homologous DNA
      sequences in vitro, and TLS(-/-) mice and embryonic fibroblasts exhibit increased
      sensitivity to ionizing irradiation. These results are consistent with a role for
      TLS in homologous DNA pairing and recombination.
FAU - Kuroda, M
AU  - Kuroda M
AD  - Skirball Institute of Biomolecular Medicine, the Departments of Medicine, Cell
      Biology and the Kaplan Cancer Center, NYU Medical Center, New York, NY 10016,
      USA.
FAU - Sok, J
AU  - Sok J
FAU - Webb, L
AU  - Webb L
FAU - Baechtold, H
AU  - Baechtold H
FAU - Urano, F
AU  - Urano F
FAU - Yin, Y
AU  - Yin Y
FAU - Chung, P
AU  - Chung P
FAU - de Rooij, D G
AU  - de Rooij DG
FAU - Akhmedov, A
AU  - Akhmedov A
FAU - Ashley, T
AU  - Ashley T
FAU - Ron, D
AU  - Ron D
LA  - eng
GR  - CA60945/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Heterogeneous-Nuclear Ribonucleoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Protein FUS)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (TATA-Binding Protein Associated Factors)
RN  - 0 (Transcription Factors)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - Chromosome Aberrations
MH  - Chromosome Disorders
MH  - Embryo, Mammalian/radiation effects
MH  - Female
MH  - Fibroblasts
MH  - Genitalia/pathology/radiation effects
MH  - Heterogeneous-Nuclear Ribonucleoproteins
MH  - Immunohistochemistry
MH  - Infertility/*genetics
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - RNA/metabolism
MH  - RNA, Messenger/drug effects
MH  - RNA-Binding Protein FUS
MH  - RNA-Binding Proteins/genetics
MH  - Radiation Tolerance/*genetics
MH  - Radiation, Ionizing
MH  - Recombination, Genetic
MH  - Ribonucleoproteins/*genetics/metabolism
MH  - Spermatozoa/pathology/radiation effects
MH  - Synaptonemal Complex/genetics
MH  - *TATA-Binding Protein Associated Factors
MH  - Testis/radiation effects
MH  - Transcription Factors/metabolism
MH  - Ultraviolet Rays
PMC - PMC305582
EDAT- 2000/02/02 09:00
MHDA- 2000/03/18 09:00
CRDT- 2000/02/02 09:00
PHST- 2000/02/02 09:00 [pubmed]
PHST- 2000/03/18 09:00 [medline]
PHST- 2000/02/02 09:00 [entrez]
AID - 10.1093/emboj/19.3.453 [doi]
PST - ppublish
SO  - EMBO J. 2000 Feb 1;19(3):453-62. doi: 10.1093/emboj/19.3.453.