PMID- 10654605
OWN - NLM
STAT- MEDLINE
DCOM- 20000303
LR  - 20220224
IS  - 0950-1991 (Print)
IS  - 0950-1991 (Linking)
VI  - 127
IP  - 1
DP  - 2000 Jan
TI  - SHH-N upregulates Sfrp2 to mediate its competitive interaction with WNT1 and WNT4
      in the somitic mesoderm.
PG  - 109-18
AB  - Dorsoventral polarity of the somitic mesoderm is established by competitive
      signals originating from adjacent tissues. The ventrally located notochord
      provides the ventralizing signals to specify the sclerotome, while the dorsally
      located surface ectoderm and dorsal neural tube provide the dorsalizing signals
      to specify the dermomyotome. Noggin and SHH-N have been implicated as the
      ventralizing signals produced by the notochord. Members of the WNT family of
      proteins, on the other hand, have been implicated as the dorsalizing signals
      derived from the ectoderm and dorsal neural tube. When presomitic explants are
      confronted with cells secreting SHH-N and WNT1 simultaneously, competition to
      specify the sclerotome and dermomyotome domains within the naive mesoderm can be 
      observed. Here, using these explant cultures, we provide evidence that SHH-N
      competes with WNT1, not only by upregulating its own receptor Ptc1, but also by
      upregulating Sfrp2 (Secreted frizzled-related protein 2), which encodes a
      potential WNT antagonist. Among the four known Sfrps, Sfrp2 is the only member
      expressed in the sclerotome and upregulated by SHH-N recombinant protein. We
      further show that SFRP2-expressing cells can reduce the dermomyotome-inducing
      activity of WNT1 and WNT4, but not that of WNT3a. Together, our results support
      the model that SHH-N at least in part employs SFRP2 to reduce WNT1/4 activity in 
      the somitic mesoderm.
FAU - Lee, C S
AU  - Lee CS
AD  - Department of Embryology, Carnegie Institution of Washington, Baltimore, MD
      21210, USA.
FAU - Buttitta, L A
AU  - Buttitta LA
FAU - May, N R
AU  - May NR
FAU - Kispert, A
AU  - Kispert A
FAU - Fan, C M
AU  - Fan CM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Development
JT  - Development (Cambridge, England)
JID - 8701744
RN  - 0 (Bmp4 protein, mouse)
RN  - 0 (Bone Morphogenetic Protein 4)
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Eye Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Hedgehog Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Sfrp2 protein, mouse)
RN  - 0 (Trans-Activators)
RN  - 0 (WD repeat containing planar cell polarity effector)
RN  - 0 (Wnt Proteins)
RN  - 0 (Wnt1 Protein)
RN  - 0 (Wnt1 protein, mouse)
RN  - 0 (Wnt4 Protein)
RN  - 0 (Wnt4 protein, mouse)
RN  - 0 (Zebrafish Proteins)
RN  - 0 (bmp4 protein, zebrafish)
RN  - 148294-77-3 (noggin protein)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Bone Morphogenetic Protein 4
MH  - Bone Morphogenetic Proteins/physiology
MH  - COS Cells
MH  - Carrier Proteins
MH  - Eye Proteins/genetics/*metabolism
MH  - *Glycoproteins
MH  - Hedgehog Proteins
MH  - Intracellular Signaling Peptides and Proteins
MH  - *Membrane Proteins
MH  - Mesoderm/*physiology
MH  - Mice
MH  - Notochord
MH  - Proteins/genetics/*metabolism/physiology
MH  - Proto-Oncogene Proteins/genetics/*metabolism
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - *Trans-Activators
MH  - Up-Regulation
MH  - Wnt Proteins
MH  - Wnt1 Protein
MH  - Wnt4 Protein
MH  - *Zebrafish Proteins
EDAT- 2000/02/02 09:00
MHDA- 2000/03/11 09:00
CRDT- 2000/02/02 09:00
PHST- 2000/02/02 09:00 [pubmed]
PHST- 2000/03/11 09:00 [medline]
PHST- 2000/02/02 09:00 [entrez]
AID - 10.1242/dev.127.1.109 [doi]
PST - ppublish
SO  - Development. 2000 Jan;127(1):109-18. doi: 10.1242/dev.127.1.109.