PMID- 10653592
OWN - NLM
STAT- MEDLINE
DCOM- 20000210
LR  - 20170214
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 48
IP  - 1
DP  - 2000 Jan
TI  - Co-expression of the squamous cell carcinoma antigens 1 and 2 in normal adult
      human tissues and squamous cell carcinomas.
PG  - 113-22
AB  - Squamous cell carcinoma antigen (SCCA) serves as a serological marker for
      advanced squamous cell carcinomas (SCCs) and as an indicator of therapeutic
      response. Recent molecular studies show that the SCCA is transcribed by two
      almost identical tandemly arrayed genes, SCCA1 and SCCA2. These genes are members
      of the high molecular weight serine proteinase inhibitor (serpin) superfamily.
      Although SCCA1 and SCCA2 are 92% identical at the amino acid level, they have
      distinct biochemical properties. Paradoxically, SCCA1 is an inhibitor of
      papain-like cysteine proteinases, such as cathepsins L, S, and K, whereas SCCA2
      inhibits chymotrypsin-like serine proteinases, cathepsin G, and mast cell
      chymase. Using a new set of discriminatory monoclonal antibodies (MAbs) and
      polymerase chain reaction (PCR) assay, we showed that SCCA1 and SCCA2 were
      co-expressed in the suprabasal layers of the stratified squamous epithelium of
      the tongue, tonsil, esophagus, uterine cervix and vagina, Hassall's corpuscles of
      the thymus, and some areas of the skin. SCCA1 and SCCA2 also were detected in the
      pseudo-stratified columnar epithelium of the conducting airways. Examination of
      squamous cell carcinomas of the lung and head and neck showed that SCCA1 and
      SCCA2 were co-expressed in moderately and well-differentiated tumors. Moreover,
      there was no differential expression between these SCCA "isoforms" in normal or
      malignant tissues. In contrast to previous studies, these data indicated that the
      expression of SCCA1 and SCCA2 was not restricted to the squamous epithelium and
      that these serpins may coordinately regulate cysteine and serine proteinase
      activity in both normal and transformed tissues.
FAU - Cataltepe, S
AU  - Cataltepe S
AD  - Division of Newborn Medicine, Children's Hospital, Beth Israel Deaconess Medical 
      Center, Harvard Medical School, Boston, Massachusetts 02115-5737, USA.
FAU - Gornstein, E R
AU  - Gornstein ER
FAU - Schick, C
AU  - Schick C
FAU - Kamachi, Y
AU  - Kamachi Y
FAU - Chatson, K
AU  - Chatson K
FAU - Fries, J
AU  - Fries J
FAU - Silverman, G A
AU  - Silverman GA
FAU - Upton, M P
AU  - Upton MP
LA  - eng
GR  - CA69331/CA/NCI NIH HHS/United States
GR  - CA73031/CA/NCI NIH HHS/United States
GR  - HD28475/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the
      Histochemistry Society
JID - 9815334
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Protein Isoforms)
RN  - 0 (Serpins)
RN  - 0 (squamous cell carcinoma-related antigen)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Antibody Specificity
MH  - Antigens, Neoplasm/genetics/*isolation & purification
MH  - Biomarkers, Tumor/genetics/*isolation & purification
MH  - Carcinoma, Squamous Cell/*chemistry/pathology
MH  - Epithelium/chemistry
MH  - Female
MH  - Humans
MH  - Pregnancy
MH  - Protein Isoforms
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Serpins/genetics/*isolation & purification
MH  - Tissue Distribution
EDAT- 2000/02/01 00:00
MHDA- 2000/02/01 00:01
CRDT- 2000/02/01 00:00
PHST- 2000/02/01 00:00 [pubmed]
PHST- 2000/02/01 00:01 [medline]
PHST- 2000/02/01 00:00 [entrez]
AID - 10.1177/002215540004800112 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 2000 Jan;48(1):113-22. doi: 10.1177/002215540004800112.