PMID- 10652346
OWN - NLM
STAT- MEDLINE
DCOM- 20000302
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 5
DP  - 2000 Feb 4
TI  - OUT, a novel basic helix-loop-helix transcription factor with an Id-like
      inhibitory activity.
PG  - 3510-21
AB  - Transcription factors belonging to the basic helix-loop-helix (bHLH) family are
      involved in various cell differentiation processes. We report the isolation and
      functional characterization of a novel bHLH factor, termed OUT. OUT, structurally
      related to capsulin/epicardin/Pod-1 and ABF-1/musculin/MyoR, is expressed mainly 
      in the adult mouse reproductive organs, such as the ovary, uterus, and testis,
      and is barely detectable in tissues of developing embryos. Physical association
      of OUT with the E protein was predicted from the primary structure of OUT and
      confirmed by co-immunoprecipitation. However, unlike other bHLH factors, this
      novel protein failed to bind E-box or N-box DNA sequences and inhibited DNA
      binding of homo- and heterodimers consisting of E12 and MyoD in gel mobility
      shift assays. In luciferase assays, OUT inhibited the induction of
      E-box-dependent transactivation by MyoD-E12 heterodimers. Deletion studies
      identified the domain responsible for the inhibitory action of OUT in its bHLH
      and C-terminal regions. Moreover, terminal differentiation of C2C12 myoblasts was
      inhibited by exogenous introduction of OUT. These inhibitory functions of OUT
      closely resemble those of the helix-loop-helix inhibitor Id proteins. Based on
      these findings, we propose that this novel protein functions as a negative
      regulator of bHLH factors through the formation of a functionally inactive
      heterodimeric complex.
FAU - Narumi, O
AU  - Narumi O
AD  - Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, 
      Shogoin Kawahara-cho 53, Sakyo-ku, 606-8507 Kyoto, Japan.
FAU - Mori, S
AU  - Mori S
FAU - Boku, S
AU  - Boku S
FAU - Tsuji, Y
AU  - Tsuji Y
FAU - Hashimoto, N
AU  - Hashimoto N
FAU - Nishikawa, S
AU  - Nishikawa S
FAU - Yokota, Y
AU  - Yokota Y
LA  - eng
SI  - GENBANK/AF142405
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (DNA, Complementary)
RN  - 0 (Tcf23 protein, mouse)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Basic Helix-Loop-Helix Transcription Factors
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics/isolation & purification
MH  - Helix-Loop-Helix Motifs/genetics
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Molecular Sequence Data
MH  - Sequence Alignment
MH  - Transcription Factors/*genetics/isolation & purification/metabolism
EDAT- 2000/02/01 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/02/01 09:00
PHST- 2000/02/01 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/02/01 09:00 [entrez]
AID - 10.1074/jbc.275.5.3510 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Feb 4;275(5):3510-21. doi: 10.1074/jbc.275.5.3510.