PMID- 10652336
OWN - NLM
STAT- MEDLINE
DCOM- 20000302
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 5
DP  - 2000 Feb 4
TI  - Identification of CD7 as a cognate of the human K12 (SECTM1) protein.
PG  - 3431-7
AB  - CD7 is a 40-kDa protein found primarily on T, NK, and pre-B cells; the function
      of the CD7 protein in the immune system is largely unknown. The K12 (SECTM1)
      protein was originally identified by its location just upstream of the CD7 locus.
      The K12 gene encodes a transmembrane protein of unknown function. In order to
      clone a K12-binding protein, we generated a soluble version of the human K12
      protein by fusing its extracellular domain to the Fc portion of human IgG(1).
      Flow cytometry experiments showed that the K12-Fc fusion protein bound at high
      levels to both human T and NK cells. Precipitation experiments using K12-Fc on
      (35)S-radiolabeled NK cells lysates indicated that the K12 cognate was an
      approximately 40-kDa protein. A human peripheral blood T cell cDNA expression
      library was screened with the K12-Fc protein, and two independent, positive cDNA 
      clones were identified and sequenced. Both cDNAs encoded the same protein, which 
      was CD7. Thus, K12 and CD7 are cognate proteins that are located next to each
      other on human chromosome 17q25. Additionally, we have cloned the gene encoding
      the mouse homologue of K12, shown that it maps near the mouse CD7 gene on
      chromosome 11, and established that the mouse K12 protein binds to mouse, but not
      human, CD7. Mouse K12-Fc inhibited in a dose-dependent manner concanavalin
      A-induced proliferation, but not anti-TcRalpha/beta induced proliferation, of
      mouse lymph node T cells. Human K12-Fc stimulated the up-regulation of CD25,
      CD54, and CD69 on human NK cells in vitro.
FAU - Lyman, S D
AU  - Lyman SD
AD  - Immunex Corp., Seattle, Washington 98101, USA. slyman@immunex.com
FAU - Escobar, S
AU  - Escobar S
FAU - Rousseau, A M
AU  - Rousseau AM
FAU - Armstrong, A
AU  - Armstrong A
FAU - Fanslow, W C
AU  - Fanslow WC
LA  - eng
SI  - GENBANK/AF210700
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Antigens, CD7)
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Proteins)
RN  - 0 (SECTM1 protein, human)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, CD7/*genetics/metabolism
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 17
MH  - Cloning, Molecular
MH  - DNA, Complementary/analysis/genetics
MH  - Humans
MH  - Membrane Proteins/*genetics/metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Sequence Alignment
MH  - T-Lymphocytes/*metabolism
EDAT- 2000/02/01 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/02/01 09:00
PHST- 2000/02/01 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/02/01 09:00 [entrez]
AID - 10.1074/jbc.275.5.3431 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Feb 4;275(5):3431-7. doi: 10.1074/jbc.275.5.3431.