PMID- 10652313
OWN - NLM
STAT- MEDLINE
DCOM- 20000302
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 5
DP  - 2000 Feb 4
TI  - NMR solution structure of complement-like repeat CR3 from the low density
      lipoprotein receptor-related protein. Evidence for specific binding to the
      receptor binding domain of human alpha(2)-macroglobulin.
PG  - 3264-9
AB  - We have used NMR methods to determine the structure of the calcium complex of
      complement-like repeat 3 (CR3) from the low density lipoprotein receptor-related 
      protein (LRP) and to examine its specific interaction with the receptor binding
      domain of human alpha(2)-macroglobulin. CR3 is one of eight related repeats that 
      constitute a major ligand binding region of LRP. The structure is very similar in
      overall fold to homologous complement-like repeat CR8 from LRP and
      complement-like repeats LB1, LB2, and LB5 from the low density lipoprotein
      receptor and contains a short two-strand antiparallel beta-sheet, a one turn
      alpha-helix, and a high affinity calcium site with coordination from four
      carboxyls and two backbone carbonyls. The surface electrostatics and topography
      are, however, quite distinct from each of these other repeats. Two-dimensional
      (1)H,(15)N-heteronuclear single quantum coherence spectra provide evidence for a 
      specific, though relatively weak (K(d) approximately 140 microM), interaction
      between CR3 and human alpha2-macroglobulin receptor binding domain that involves 
      a contiguous patch of surface residues in the central region of CR3. This
      specific interaction is consistent with a mode of LRP binding to ligands that
      uses contributions from more than one domain to generate a wide array of
      different binding sites, each with overall high affinity.
FAU - Dolmer, K
AU  - Dolmer K
AD  - Department of Biochemistry, College of Medicine, University of Illinois at
      Chicago, Chicago, Illinois 60612-4316, USA.
FAU - Huang, W
AU  - Huang W
FAU - Gettins, P G
AU  - Gettins PG
LA  - eng
SI  - PDB/1 D21
GR  - GM54414/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Low Density Lipoprotein Receptor-Related Protein-1)
RN  - 0 (Macrophage-1 Antigen)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (alpha-Macroglobulins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Binding Sites
MH  - Humans
MH  - Low Density Lipoprotein Receptor-Related Protein-1
MH  - Macrophage-1 Antigen/chemistry
MH  - Magnetic Resonance Spectroscopy
MH  - Molecular Sequence Data
MH  - Receptors, Immunologic/*chemistry/metabolism
MH  - alpha-Macroglobulins/chemistry/metabolism
EDAT- 2000/02/01 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/02/01 09:00
PHST- 2000/02/01 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/02/01 09:00 [entrez]
AID - 10.1074/jbc.275.5.3264 [doi]
AID - S0021-9258(18)30902-5 [pii]
PST - ppublish
SO  - J Biol Chem. 2000 Feb 4;275(5):3264-9. doi: 10.1074/jbc.275.5.3264.