PMID- 10652257
OWN - NLM
STAT- MEDLINE
DCOM- 20000412
LR  - 20161124
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 113 ( Pt 4)
DP  - 2000 Feb
TI  - Tyrosine 766 in the fibroblast growth factor receptor-1 is required for
      FGF-stimulation of phospholipase C, phospholipase D, phospholipase A(2),
      phosphoinositide 3-kinase and cytoskeletal reorganisation in porcine aortic
      endothelial cells.
PG  - 643-51
AB  - Fibroblast growth factor-mediated signalling was studied in porcine aortic
      endothelial cells expressing either wild-type fibroblast growth factor receptor-1
      or a mutant receptor (Y766F) unable to bind phospholipase C-(&ggr;). Stimulation 
      of cells expressing the wild-type receptor resulted in activation of
      phospholipases C, D and A(2) and increased phosphoinositide 3-kinase activity.
      Stimulation of the wild-type receptor also resulted in stress fibre formation and
      a cellular shape change. Cells expressing the Y766F mutant receptor failed to
      stimulate phospholipase C, D and A(2) as well as phosphoinositide 3-kinase.
      Furthermore, no stress fibre formation or shape change was observed. Both the
      wild-type and Y766F receptor mutant activated MAP kinase and elicited
      proliferative responses in the porcine aortic endothelial cells. Thus, fibroblast
      growth factor receptor-1 mediated activation of phospholipases C, D and A(2) and 
      phosphoinositide 3-kinase was dependent on tyrosine 766. Furthermore, whilst
      tyrosine 766 was not required for a proliferative response, it was required for
      fibroblast growth factor receptor-1 mediated cytoskeletal reorganisation.
FAU - Cross, M J
AU  - Cross MJ
AD  - Department of Genetics, Rudbeck Laboratory, S-75185 Uppsala, Sweden.
      michael.cross@genpat.uu.se
FAU - Hodgkin, M N
AU  - Hodgkin MN
FAU - Roberts, S
AU  - Roberts S
FAU - Landgren, E
AU  - Landgren E
FAU - Wakelam, M J
AU  - Wakelam MJ
FAU - Claesson-Welsh, L
AU  - Claesson-Welsh L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Receptors, Fibroblast Growth Factor)
RN  - 42HK56048U (Tyrosine)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor, Fibroblast Growth Factor, Type 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.1.- (Phospholipases)
RN  - EC 3.1.1.32 (Phospholipases A)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.1.4.4 (Phospholipase D)
SB  - IM
MH  - Animals
MH  - Aorta/cytology
MH  - Cell Division/physiology
MH  - Cells, Cultured
MH  - Cytoskeleton/*metabolism
MH  - Endothelium, Vascular/cytology/*enzymology
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Mutagenesis/physiology
MH  - Phosphatidylinositol 3-Kinases/*metabolism
MH  - Phospholipase D/metabolism
MH  - Phospholipases/*metabolism
MH  - Phospholipases A/metabolism
MH  - Phosphorylation
MH  - Receptor Protein-Tyrosine Kinases/*genetics/metabolism
MH  - Receptor, Fibroblast Growth Factor, Type 1
MH  - Receptors, Fibroblast Growth Factor/*genetics/metabolism
MH  - Signal Transduction/physiology
MH  - Swine
MH  - Transfection
MH  - Type C Phospholipases/metabolism
MH  - Tyrosine/genetics
EDAT- 2000/02/01 09:00
MHDA- 2000/04/15 09:00
CRDT- 2000/02/01 09:00
PHST- 2000/02/01 09:00 [pubmed]
PHST- 2000/04/15 09:00 [medline]
PHST- 2000/02/01 09:00 [entrez]
PST - ppublish
SO  - J Cell Sci. 2000 Feb;113 ( Pt 4):643-51.