PMID- 10651948
OWN - NLM
STAT- MEDLINE
DCOM- 20000210
LR  - 20190513
IS  - 0019-2805 (Print)
IS  - 0019-2805 (Linking)
VI  - 99
IP  - 1
DP  - 2000 Jan
TI  - Role of beta1 and beta2 subunits of the interleukin-12 receptor in determining T 
      helper 1/T helper 2 responses in vivo in the rat.
PG  - 109-12
AB  - Interleukin-12 (IL-12) responsiveness, and hence capacity to mount a T helper
      type 1(Th1) immune response, may be regulated via differential expression of the 
      IL-12 receptor beta2 subunit at least in vitro in human and murine cells. To test
      whether a similar phenomenon operates in vivo in the rat we cloned and sequenced 
      partial cDNAs for rat IL-12Rbeta1 and IL-12Rbeta2 subunits and analysed
      expression of these genes in vivo in two rat strains with different Th1/Th2 bias.
      After treatment with mercuric chloride (HgCl2), Brown-Norway rats develop
      Th2-biased autoimmunity whereas Lewis rats do not develop autoimmunity, instead
      becoming resistant to Th1-biased diseases to which they are normally susceptible.
      We report close sequence homology between the segments of the rat IL-12R genes
      sequenced and corresponding mouse genes (95.6% and 92% for IL-12Rbeta1 and
      IL-12Rbeta2, respectively). Both Brown-Norway and Lewis rats express both beta1
      and beta2 subunits of IL-12 receptor in vivo in spleen; Brown-Norway rats express
      the beta2 subunit at a lower level than Lewis rats. After HgCl2 treatment,
      IL-12Rbeta1 expression was not altered but there was down-regulation of
      IL-12Rbeta2 expression in both strains. We conclude that relative
      under-expression of IL-12Rbeta2 by Brown-Norway rats contributes to their Th2
      bias, and that down-regulation of IL-12Rbeta2 after HgCl2 administration in Lewis
      rats underlies subsequent resistance to induction of Th1-biased diseases.
FAU - Gillespie, K M
AU  - Gillespie KM
AD  - Academic Renal Unit, and *Diabetes and Metabolism Unit, University of Bristol,
      Southmead Hospital, Bristol, UK.
FAU - Szeto, C C
AU  - Szeto CC
FAU - Betin, V M
AU  - Betin VM
FAU - Mathieson, P W
AU  - Mathieson PW
LA  - eng
PT  - Journal Article
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (IL12RB1 protein, human)
RN  - 0 (IL12RB2 protein, human)
RN  - 0 (Il12rb1 protein, mouse)
RN  - 0 (Il12rb1 protein, rat)
RN  - 0 (Il12rb2 protein, mouse)
RN  - 0 (Il12rb2 protein, rat)
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-12)
RN  - 187348-17-0 (Interleukin-12)
RN  - 53GH7MZT1R (Mercuric Chloride)
SB  - IM
SB  - X
MH  - Animals
MH  - Autoimmunity
MH  - Base Sequence
MH  - Gene Expression
MH  - Interleukin-12/*metabolism
MH  - Mercuric Chloride/pharmacology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Protein Isoforms/genetics
MH  - Rats
MH  - Rats, Inbred BN
MH  - Rats, Inbred Lew
MH  - Receptors, Interleukin/*genetics
MH  - Receptors, Interleukin-12
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Nucleic Acid
MH  - Spleen/*immunology
MH  - Th1 Cells/*immunology
MH  - Th2 Cells/*immunology
PMC - PMC2327121
EDAT- 2000/01/29 00:00
MHDA- 2000/01/29 00:01
CRDT- 2000/01/29 00:00
PHST- 2000/01/29 00:00 [pubmed]
PHST- 2000/01/29 00:01 [medline]
PHST- 2000/01/29 00:00 [entrez]
AID - imm927 [pii]
AID - 10.1046/j.1365-2567.2000.00927.x [doi]
PST - ppublish
SO  - Immunology. 2000 Jan;99(1):109-12. doi: 10.1046/j.1365-2567.2000.00927.x.