PMID- 10651944
OWN - NLM
STAT- MEDLINE
DCOM- 20000210
LR  - 20190513
IS  - 0019-2805 (Print)
IS  - 0019-2805 (Linking)
VI  - 99
IP  - 1
DP  - 2000 Jan
TI  - Molecular cloning, genomic organization and cell-binding characteristics of mouse
      Spalpha.
PG  - 78-86
AB  - Several group B scavenger receptor cysteine-rich (SRCR) proteins have been shown 
      to function as modulators in the immune response. Recently, we reported the
      cloning of a new member of this family, human Spalpha (hSpalpha). Herein we
      report the cloning and characterization of the mouse homologue of hSpalpha. Like 
      its human counterpart, mouse Spalpha (mSpalpha), is a secreted protein containing
      three SRCR domains. Most lymphoid tissues express RNA transcripts encoding
      mSpalpha. Characterization of a genomic clone encoding the mature mSpalpha
      protein showed that each of the SRCR domains of mSpalpha is encoded by a single
      exon. Comparison of the sequence of mSPalpha with those of other published
      proteins indicates that it is the same as the recently reported protein named AIM
      (apoptosis inhibitor expressed by macrophages). Cell-binding studies with a
      mSpalpha immunoglobulin (mSpalpha-Rgamma) fusion protein indicated that mSpalpha 
      is capable of binding to spleen-derived CD19+ B cells and minimally to peritoneal
      cavity-derived CD19+ B cells but not to peripheral blood-derived B cells.
      Spleen-derived CD3+ T cells also bound mSpalpha-Rgamma; however, no binding was
      observed to either peripheral blood mononuclear cells or peritoneal
      cavity-derived CD3+ T cells. The mSpalpha-Rgamma fusion protein was also shown to
      bind to the mouse cell lines WEHI3 (monocytic) and EL-4 (thymoma, T cell). The
      cloning of cDNA and genomic clones encoding mSpalpha and the identification of
      cells expressing a putative mSpalpha receptor(s) should facilitate in vivo
      studies designed to investigate the function of Spalpha in the immune
      compartment.
FAU - Gebe, J A
AU  - Gebe JA
AD  - The Bristol-Myers Squibb, Pharmaceutical Research Institute, Princeton, NJ
      08543-4000, USA.
FAU - Llewellyn, M
AU  - Llewellyn M
FAU - Hoggatt, H
AU  - Hoggatt H
FAU - Aruffo, A
AU  - Aruffo A
LA  - eng
SI  - GENBANK/AF018268
SI  - GENBANK/AF018269
PT  - Journal Article
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Antigens, CD19)
RN  - 0 (Bacterial Proteins)
RN  - 0 (CD3 Complex)
RN  - 0 (HspX protein, Mycobacterium tuberculosis)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Antigens, Bacterial
MH  - Antigens, CD19
MH  - B-Lymphocytes/metabolism
MH  - Bacterial Proteins/*genetics
MH  - Base Sequence
MH  - CD3 Complex
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Conserved Sequence
MH  - Flow Cytometry
MH  - Humans
MH  - Introns
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - Protein Binding
MH  - Sequence Alignment
MH  - Spleen/immunology
MH  - Thymoma/immunology
PMC - PMC2327131
EDAT- 2000/01/29 00:00
MHDA- 2000/01/29 00:01
CRDT- 2000/01/29 00:00
PHST- 2000/01/29 00:00 [pubmed]
PHST- 2000/01/29 00:01 [medline]
PHST- 2000/01/29 00:00 [entrez]
AID - imm903 [pii]
AID - 10.1046/j.1365-2567.2000.00903.x [doi]
PST - ppublish
SO  - Immunology. 2000 Jan;99(1):78-86. doi: 10.1046/j.1365-2567.2000.00903.x.