PMID- 10651871
OWN - NLM
STAT- MEDLINE
DCOM- 20000316
LR  - 20190815
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 12
IP  - 1
DP  - 2000 Jan
TI  - Different spatiotemporal expression of DOC2 genes in the developing rat brain
      argues for an additional, nonsynaptic role of DOC2B in early development.
PG  - 165-71
AB  - DOC2A and DOC2B are two homologous genes implicated in synaptic vesicle
      exocytosis. Their complementary, nonoverlapping expression patterns in adult rat 
      brain suggest that they exert similar functions in different neurons. We have
      analysed the expression pattern of the two genes in the developing rat brain by
      in situ hybridization. Unexpectedly, we found no parallel expression of the two
      genes during development. DOC2B mRNA was highly expressed as early as embryonic
      day 12 (E12) throughout the neuroepithelium, long before synaptic transmission is
      functional, and the expression remained abundant from E12 onwards. In contrast,
      faint expression of DOC2A transcripts was first detected at E17 in ventral brain 
      areas, and it extended gradually to other brain structures in the sequence of
      their ontology, i.e. structures that had formed first also expressed DOC2A first.
      At postnatal day 3, both genes were highly expressed throughout the brain. This
      overlapping expression diverged to the complementary distribution of the adult
      brain. The temporal and spatial differences in expression point to a functional
      divergence between these homologous genes during brain development: the pattern
      of DOC2A is consistent with its proposed synaptic function, whereas that of DOC2B
      suggests an additional, nonsynaptic role in proliferating cells.
FAU - Korteweg, N
AU  - Korteweg N
AD  - Rudolf Magnus Institute for Neurosciences, Utrecht University, Universiteitsweg
      100, 3584 CG Utrecht, the Netherlands.
FAU - Denekamp, F A
AU  - Denekamp FA
FAU - Verhage, M
AU  - Verhage M
FAU - Burbach, J P
AU  - Burbach JP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Doc2a protein, rat)
RN  - 0 (Doc2b protein, rat)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Aging
MH  - Animals
MH  - Brain/embryology/growth & development/*metabolism
MH  - Calcium-Binding Proteins/*genetics
MH  - *Embryonic and Fetal Development
MH  - Female
MH  - *Gene Expression Regulation, Developmental
MH  - Hippocampus/metabolism
MH  - In Situ Hybridization
MH  - Male
MH  - Neocortex/metabolism
MH  - Nerve Tissue Proteins/*genetics
MH  - Organ Specificity
MH  - Protein Isoforms/genetics
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - *Transcription, Genetic
EDAT- 2000/01/29 09:00
MHDA- 2000/03/18 09:00
CRDT- 2000/01/29 09:00
PHST- 2000/01/29 09:00 [pubmed]
PHST- 2000/03/18 09:00 [medline]
PHST- 2000/01/29 09:00 [entrez]
AID - ejn898 [pii]
AID - 10.1046/j.1460-9568.2000.00898.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2000 Jan;12(1):165-71. doi: 10.1046/j.1460-9568.2000.00898.x.