PMID- 10651863
OWN - NLM
STAT- MEDLINE
DCOM- 20000316
LR  - 20190815
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 12
IP  - 1
DP  - 2000 Jan
TI  - Distinct immunohistochemical localization of two isoforms of
      Ca2+/calmodulin-dependent protein kinase kinases in the adult rat brain.
PG  - 89-99
AB  - Ca2+/calmodulin-dependent protein kinase IV (CaM-KIV) is thought to be involved
      in regulating gene expression by phosphorylating various transcriptional factors.
      CaM-KIV as well as CaM-KI are activated upon phosphorylation by two distinct
      isoforms of Ca2+/calmodulin-dependent protein kinase kinases, CaM-KKs alpha and
      beta. In this study, we raised isoform-specific monoclonal antibodies against
      CaM-KKs and examined the immunohistochemical localization of CaM-KKs in the rat
      brain, compared with that of CaM-KIV. CaM-KK alpha-immunoreactivity was rather
      widely distributed in neurons throughout the brain, except cerebellar cortex. The
      highest levels of CaM-KK alpha-immunoreactivity were observed in the cerebral
      cortex, facial nucleus and motor neurons of the spinal cord. Moderate CaM-KK
      alpha-immunoreactivity was observed in the hippocampal formation, pontine nuclei 
      and various brain stem nuclei including trigeminal, vestibular, cochlear and
      hypoglossal nuclei. In contrast, CaM-KK beta-immunoreactivity was relatively
      restricted in some neuronal populations. The highest levels of CaM-KK
      beta-immunoreactivity were observed in the cerebellar granule cell layer, and
      moderate immunoreactivity was observed in the cerebral cortex, hippocampal
      formation, caudate putamen, pontine nuclei, cochlear nucleus and molecular layer 
      of the cerebellum. In contrast to the prominent nuclear localization of CaM-KIV, 
      both isoforms of CaM-KKs were localized in the perikaryal cytoplasm, dendrites
      and nerve terminals, but not in the cell nuclei. The distinct localization of two
      isoforms of CaM-KKs suggests that the complicated mechanisms for activation of
      CaM-KIV by CaM-KKs may be exerted in region-specific manners as well as
      intracellularly.
FAU - Sakagami, H
AU  - Sakagami H
AD  - Division of Histology, Department of Cell Biology, Graduate School of Medical
      Sciences, Tohoku University, Sendai 980-0782, Japan.
      hsakagam@gonryo.med.tohoku.ac.jp
FAU - Umemiya, M
AU  - Umemiya M
FAU - Saito, S
AU  - Saito S
FAU - Kondo, H
AU  - Kondo H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Isoenzymes)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Kinase)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 4)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.17 (Camk4 protein, mouse)
RN  - EC 2.7.11.17 (Camk4 protein, rat)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Brain/cytology/*enzymology
MH  - COS Cells
MH  - Calcium-Calmodulin-Dependent Protein Kinase Kinase
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 4
MH  - Calcium-Calmodulin-Dependent Protein Kinases/*analysis
MH  - Hybrid Cells
MH  - Immunohistochemistry
MH  - Isoenzymes/analysis
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neurons/*enzymology
MH  - Protein-Serine-Threonine Kinases/*analysis
MH  - Rats
MH  - Rats, Wistar
MH  - Recombinant Proteins/analysis
MH  - Subcellular Fractions/enzymology
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 2000/01/29 09:00
MHDA- 2000/03/18 09:00
CRDT- 2000/01/29 09:00
PHST- 2000/01/29 09:00 [pubmed]
PHST- 2000/03/18 09:00 [medline]
PHST- 2000/01/29 09:00 [entrez]
AID - ejn883 [pii]
AID - 10.1046/j.1460-9568.2000.00883.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2000 Jan;12(1):89-99. doi: 10.1046/j.1460-9568.2000.00883.x.