PMID- 10651831
OWN - NLM
STAT- MEDLINE
DCOM- 20000308
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 267
IP  - 3
DP  - 2000 Feb
TI  - Molecular characterization of a human scavenger receptor, human MARCO.
PG  - 919-26
AB  - Murine MARCO has been identified recently in subsets of macrophages located in
      the peritoneum, marginal zone of the spleen, and the medullary cord of lymph
      nodes, where it has been proposed that it serves as a bacteria-binding receptor. 
      A scavenger receptor family member with an extended collagenous domain, murine
      MARCO has also been demonstrated in atherosclerotic lesions of susceptible mice. 
      We report here the identification, tissue and chromosomal localization, and
      pharmacological characterization of human (h)MARCO. hMARCO was identified from a 
      macrophage cDNA library by electronic screening with the murine MARCO sequence.
      Nucleotide sequence analysis confirmed that the full-length hMARCO clone encoded 
      a 519-amino acid protein sharing 68.5% identity with murine MARCO. RNA blot
      analysis indicated that the hMARCO transcript is 2.0 kb in length and is
      predominantly expressed in human lung, liver, and lymph nodes. Radiation hybrid
      mapping localized hMARCO to chromosome 2q14. Ligand-binding studies of COS cells 
      expressing hMARCO demonstrated significant specific binding of both Escherichia
      coli and Staphylococcus aureus. In contrast, the hMARCO receptor expressed in COS
      cells did not specifically bind the scavenger receptor ligand acetylated
      low-density lipoprotein (LDL), despite its similarity to the elongated
      collagen-like binding domain of the macrophage scavenger receptor. In addition,
      acetylated (Ac)LDL and oxidized (Ox)LDL did not inhibit E. coli binding to
      hMARCO. These data suggest that hMARCO may play an important role in host
      defense, but it has no obvious role in the accumulation of modified lipoproteins 
      during atherogenesis.
FAU - Elshourbagy, N A
AU  - Elshourbagy NA
AD  - Department of Molecular Biology, SmithKline Beecham Pharmaceuticals, King of
      Prussia, PA, USA. Nabil_A_Elshourbagy@sbphrd.com
FAU - Li, X
AU  - Li X
FAU - Terrett, J
AU  - Terrett J
FAU - Vanhorn, S
AU  - Vanhorn S
FAU - Gross, M S
AU  - Gross MS
FAU - Adamou, J E
AU  - Adamou JE
FAU - Anderson, K M
AU  - Anderson KM
FAU - Webb, C L
AU  - Webb CL
FAU - Lysko, P G
AU  - Lysko PG
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (DNA Primers)
RN  - 0 (MARCO protein, human)
RN  - 0 (Marco protein, mouse)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Bacterial Adhesion
MH  - Base Sequence
MH  - COS Cells
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 2/genetics
MH  - Cloning, Molecular
MH  - DNA Primers/genetics
MH  - Escherichia coli/immunology
MH  - Female
MH  - Humans
MH  - Macrophages/immunology/*metabolism/microbiology
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptors, Immunologic/*genetics/*metabolism
MH  - Recombinant Proteins/genetics/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Species Specificity
MH  - Tissue Distribution
EDAT- 2000/01/29 09:00
MHDA- 2000/03/11 09:00
CRDT- 2000/01/29 09:00
PHST- 2000/01/29 09:00 [pubmed]
PHST- 2000/03/11 09:00 [medline]
PHST- 2000/01/29 09:00 [entrez]
AID - ejb1077 [pii]
AID - 10.1046/j.1432-1327.2000.01077.x [doi]
PST - ppublish
SO  - Eur J Biochem. 2000 Feb;267(3):919-26. doi: 10.1046/j.1432-1327.2000.01077.x.