PMID- 10649566
OWN - NLM
STAT- MEDLINE
DCOM- 20000407
LR  - 20131121
IS  - 1097-6256 (Print)
IS  - 1097-6256 (Linking)
VI  - 3
IP  - 2
DP  - 2000 Feb
TI  - Identification and characterization of the high-affinity choline transporter.
PG  - 120-5
AB  - In cholinergic neurons, high-affinity choline uptake in presynaptic terminals is 
      the rate-limiting step in acetylcholine synthesis. Using information provided by 
      the Caenorhabditis elegans Genome Project, we cloned a cDNA encoding the
      high-affinity choline transporter from C. elegans (cho-1). We subsequently used
      this clone to isolate the corresponding cDNA from rat (CHT1). CHT1 is not
      homologous to neurotransmitter transporters, but is homologous to members of the 
      Na+-dependent glucose transporter family. Expression of CHT1 mRNA is restricted
      to cholinergic neurons. The characteristics of CHT1-mediated choline uptake
      essentially match those of high-affinity choline uptake in rat brain
      synaptosomes.
FAU - Okuda, T
AU  - Okuda T
AD  - Department of Neurochemistry, Faculty of Medicine, University of Tokyo and CREST 
      of Japan Science and Technology Corporation, Bunkyo-ku, Tokyo 113-0033, Japan.
      okuda@m.u-tokyo.ac.jp
FAU - Haga, T
AU  - Haga T
FAU - Kanai, Y
AU  - Kanai Y
FAU - Endou, H
AU  - Endou H
FAU - Ishihara, T
AU  - Ishihara T
FAU - Katsura, I
AU  - Katsura I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Nat Neurosci
JT  - Nature neuroscience
JID - 9809671
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Monosaccharide Transport Proteins)
RN  - 0 (Neurotransmitter Uptake Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 0 (choline transporter)
RN  - 312-45-8 (Hemicholinium 3)
RN  - 9NEZ333N27 (Sodium)
RN  - N91BDP6H0X (Choline)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding, Competitive/drug effects
MH  - CHO Cells
MH  - Caenorhabditis elegans
MH  - Carrier Proteins/*chemistry/*genetics/metabolism
MH  - Cell Membrane/metabolism
MH  - Choline/metabolism/pharmacokinetics
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - DNA, Complementary/genetics
MH  - Hemicholinium 3/pharmacology
MH  - *Membrane Transport Proteins
MH  - Molecular Sequence Data
MH  - Monosaccharide Transport Proteins/genetics
MH  - Neurons/metabolism
MH  - Neurotransmitter Uptake Inhibitors/pharmacology
MH  - Oocytes/cytology/metabolism
MH  - Organ Specificity
MH  - Phylogeny
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Sequence Homology, Amino Acid
MH  - Sodium/metabolism
EDAT- 2000/01/29 09:00
MHDA- 2001/03/23 10:01
CRDT- 2000/01/29 09:00
PHST- 2000/01/29 09:00 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 2000/01/29 09:00 [entrez]
AID - 10.1038/72059 [doi]
PST - ppublish
SO  - Nat Neurosci. 2000 Feb;3(2):120-5. doi: 10.1038/72059.