PMID- 10648831
OWN - NLM
STAT- MEDLINE
DCOM- 20000222
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 466
IP  - 1
DP  - 2000 Jan 21
TI  - Isolation and characterisation of the human rab18 gene after stimulation of
      endothelial cells with histamine.
PG  - 148-54
AB  - Here we report the isolation of a cDNA encoding the complete human rab18 protein 
      from histamine-stimulated endothelial cells using differential display. The amino
      acid sequence showed 98% homology with the previously isolated mouse rab18
      protein, which is implicated in apical/basolateral endocytosis. Northern blot
      analysis revealed two transcripts (2.5 kb and 1 kb) ubiquitously expressed in all
      examined organs, as well as in human umbilical vein endothelial and aortic cells.
      In blood cells rab18 transcripts were hardly detectable. The histamine-induced
      time-dependent increase of rab18 mRNA expression in human umbilical vein
      endothelial cells and peripheral blood mononuclear cells indicates for the first 
      time a link between receptor-mediated signal transduction and the regulation of
      rab gene expression. This finding might also imply a role for rab proteins in
      inflammation.
FAU - Schafer, U
AU  - Schafer U
AD  - Biochemical and Experimental Division, II. Department of Surgery, University of
      Cologne, Ostmerheimer Str. 200, 51109, Cologne, Germany. u.schaefer@uni-koeln.de
FAU - Seibold, S
AU  - Seibold S
FAU - Schneider, A
AU  - Schneider A
FAU - Neugebauer, E
AU  - Neugebauer E
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (RAB18 protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (Rab18 protein, mouse)
RN  - 820484N8I3 (Histamine)
RN  - EC 3.6.5.2 (rab GTP-Binding Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - DNA Primers/genetics
MH  - DNA, Complementary/genetics/isolation & purification
MH  - Endothelium, Vascular/drug effects/metabolism
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Histamine/pharmacology
MH  - Humans
MH  - In Vitro Techniques
MH  - Leukocytes, Mononuclear/drug effects/metabolism
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - RNA, Messenger/genetics/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Signal Transduction
MH  - rab GTP-Binding Proteins/*genetics
EDAT- 2000/01/29 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/01/29 09:00
PHST- 2000/01/29 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/01/29 09:00 [entrez]
AID - S0014-5793(99)01778-0 [pii]
AID - 10.1016/s0014-5793(99)01778-0 [doi]
PST - ppublish
SO  - FEBS Lett. 2000 Jan 21;466(1):148-54. doi: 10.1016/s0014-5793(99)01778-0.