PMID- 10648811
OWN - NLM
STAT- MEDLINE
DCOM- 20000222
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 466
IP  - 1
DP  - 2000 Jan 21
TI  - Molecular cloning and functional expression of Ca(v)3.1c, a T-type calcium
      channel from human brain.
PG  - 54-8
AB  - Low voltage-activated T-type calcium channels are encoded by a family of at least
      three genes, with additional diversity created by alternative splicing. This
      study describes the cloning of the human brain alpha1G, which is a novel isoform,
      Ca(v)3.1c. Comparison of this sequence to genomic sequences deposited in the
      GenBank allowed us to identify the intron/exon boundaries of the human CACNA1G
      gene. A full-length cDNA was constructed, then used to generate a
      stably-transfected mammalian cell line. The resulting currents were analyzed for 
      their voltage- and time-dependent properties. These properties identify this gene
      as encoding a T-type Ca(2+) channel.
FAU - Cribbs, L L
AU  - Cribbs LL
AD  - Department of Physiology and the Cardiovascular Institute, Loyola University
      Medical Center, Maywood, IL, USA.
FAU - Gomora, J C
AU  - Gomora JC
FAU - Daud, A N
AU  - Daud AN
FAU - Lee, J H
AU  - Lee JH
FAU - Perez-Reyes, E
AU  - Perez-Reyes E
LA  - eng
GR  - HL58728/HL/NHLBI NIH HHS/United States
GR  - NS38691/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (CACNA1G protein, human)
RN  - 0 (Cacna1g protein, rat)
RN  - 0 (Calcium Channels, T-Type)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
SB  - IM
EIN - FEBS Lett 2000 Mar 31;470(3):378
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Brain/*metabolism
MH  - Calcium Channels, T-Type/*genetics/*metabolism
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA Primers/genetics
MH  - DNA, Complementary/genetics
MH  - Exons
MH  - Gene Expression
MH  - Humans
MH  - Introns
MH  - Kinetics
MH  - Membrane Potentials
MH  - Molecular Sequence Data
MH  - Rats
MH  - Transfection
EDAT- 2000/01/29 09:00
MHDA- 2000/05/29 09:00
CRDT- 2000/01/29 09:00
PHST- 2000/01/29 09:00 [pubmed]
PHST- 2000/05/29 09:00 [medline]
PHST- 2000/01/29 09:00 [entrez]
AID - S0014-5793(99)01756-1 [pii]
AID - 10.1016/s0014-5793(99)01756-1 [doi]
PST - ppublish
SO  - FEBS Lett. 2000 Jan 21;466(1):54-8. doi: 10.1016/s0014-5793(99)01756-1.