PMID- 10648427
OWN - NLM
STAT- MEDLINE
DCOM- 20000224
LR  - 20081121
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 95
IP  - 3
DP  - 2000 Feb 1
TI  - Cloning of the cellular receptor for feline leukemia virus subgroup C (FeLV-C), a
      retrovirus that induces red cell aplasia.
PG  - 1093-9
AB  - Feline leukemia virus-C (FeLV-C) causes red cell aplasia in cats, likely through 
      its interaction with its cell surface receptor. We identified this receptor by
      the functional screening of a library of complementary DNAs (cDNA) from feline T 
      cells. The library, which was cloned into a retroviral vector, was introduced
      into FeLV-C-resistant murine (NIH 3T3) cells. The gene conferring susceptibility 
      to FeLV-C was isolated and reintroduced into the same cell type, as well as into 
      FeLV-C-resistant rat (NRK 52E) cells, to verify its role in viral infection. The 
      receptor cDNA is predicted to encode a protein of 560 amino acids with 12
      membrane-spanning domains, termed FLVCR. FLVCR has significant amino acid
      sequence homology with members of the major facilitator superfamily and
      especially D-glucarate transporters described in bacteria and in C. elegans. As
      FeLV-C impairs the in vivo differentiation of burst-forming unit-erythroid to
      colony-forming unit-erythroid, we hypothesize that this transporter system could 
      have an essential role in early erythropoiesis. In further studies, a 6-kb
      fragment of the human FLVCR gene was amplified by polymerase chain reaction from 
      genomic DNA, using homologous cDNA sequences identified in the human Expressed
      Sequence Tags database. By radiation hybrid mapping, the human gene was localized
      to a 0.5-centiMorgan region on the long arm of chromosome 1 at q31.3.
FAU - Quigley, J G
AU  - Quigley JG
AD  - Division of Hematology, Department of Medicine, University of Washington, Seattle
      98195-7710, USA.
FAU - Burns, C C
AU  - Burns CC
FAU - Anderson, M M
AU  - Anderson MM
FAU - Lynch, E D
AU  - Lynch ED
FAU - Sabo, K M
AU  - Sabo KM
FAU - Overbaugh, J
AU  - Overbaugh J
FAU - Abkowitz, J L
AU  - Abkowitz JL
LA  - eng
GR  - CA51080/CA/NCI NIH HHS/United States
GR  - R01 HL31823/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Helminth Proteins)
RN  - 0 (Receptors, Virus)
RN  - 0 (feline leukemia virus receptor)
SB  - AIM
SB  - IM
EIN - Blood 2000 Jul 1;96(1):8
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Bacterial Proteins/chemistry/genetics
MH  - Caenorhabditis elegans/genetics
MH  - Cats/*genetics
MH  - Cell Line
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 1/genetics
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Erythroid Precursor Cells/pathology
MH  - Erythropoiesis/genetics
MH  - Escherichia coli/genetics
MH  - Expressed Sequence Tags
MH  - Genetic Predisposition to Disease
MH  - Helminth Proteins/chemistry/genetics
MH  - Humans
MH  - Hybrid Cells
MH  - Leukemia Virus, Feline/metabolism/*pathogenicity
MH  - Mice
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Rats
MH  - Receptors, Virus/biosynthesis/*genetics
MH  - Red-Cell Aplasia, Pure/*etiology
MH  - Retroviridae Infections/*complications
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Species Specificity
EDAT- 2000/01/29 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/01/29 09:00
PHST- 2000/01/29 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/01/29 09:00 [entrez]
PST - ppublish
SO  - Blood. 2000 Feb 1;95(3):1093-9.