PMID- 10648410
OWN - NLM
STAT- MEDLINE
DCOM- 20000224
LR  - 20171116
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 95
IP  - 3
DP  - 2000 Feb 1
TI  - Switch in the protein tyrosine phosphatase associated with human CD100 semaphorin
      at terminal B-cell differentiation stage.
PG  - 965-72
AB  - Human CD100, the first semaphorin identified in the immune system, is a
      transmembrane protein involved in T-cell activation. In the present study, we
      showed that activation of peripheral blood or tonsillar B lymphocytes induced the
      expression of CD100 in CD38(+)CD138(-) cell populations, including in CD148(+)
      subpopulations, thus expressing a memory B-cell-like phenotype. Using an in vitro
      enzymatic assay, we found that protein tyrosine phosphatase (PTP) activities were
      immunoprecipitated with CD100 in these cell populations, which were isolated by
      cell sorting, as well as in most B-cell lines representing various stages of
      B-cell differentiation. Immunodepletion and Western blotting experiments
      demonstrated that CD45 was the PTP associated with CD100 in cell lines displaying
      pre-B, activated B, and pre-plasma cell phenotypes. CD45 also accounted for PTP
      activity immunoprecipitated with CD100 in CD38(+)CD138(-) cells sorted after
      activation of peripheral blood or tonsillar B lymphocytes. In contrast, no
      CD100-CD45 association was observed in plasma cell lines corresponding to the
      terminal B-cell differentiation stage. CD148, the other transmembrane PTP known
      to be implicated in lymphocyte signaling pathways, was either only partly
      involved in the CD100-associated PTP activity or not expressed in plasma cell
      lines, indicating the association of CD100 with another main PTP. Our data show
      that CD100 is differentially expressed and can functionally associate with
      distinct PTPs in B cells depending on their activation and maturation state. They
      also provide evidence for a switch in the CD100-associated PTP at terminal stage 
      of B-cell differentiation.
FAU - Billard, C
AU  - Billard C
AD  - Unit 448, INSERM, Faculte de Medecine Henri Mondor, Creteil, France.
FAU - Delaire, S
AU  - Delaire S
FAU - Raffoux, E
AU  - Raffoux E
FAU - Bensussan, A
AU  - Bensussan A
FAU - Boumsell, L
AU  - Boumsell L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (CD100 antigen)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Semaphorins)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
RN  - EC 3.1.3.48 (PTPRJ protein, human)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
RN  - EC 3.1.3.48 (Receptor-Like Protein Tyrosine Phosphatases, Class 3)
RN  - EC 3.2.2.5 (ADP-ribosyl Cyclase)
RN  - EC 3.2.2.5 (CD38 protein, human)
RN  - EC 3.2.2.5 (NAD+ Nucleosidase)
RN  - EC 3.2.2.6 (ADP-ribosyl Cyclase 1)
SB  - AIM
SB  - IM
MH  - ADP-ribosyl Cyclase
MH  - ADP-ribosyl Cyclase 1
MH  - *Antigens, CD
MH  - Antigens, Differentiation/analysis
MH  - B-Lymphocytes/cytology/*metabolism
MH  - Cell Differentiation
MH  - Cell Line
MH  - Gene Expression Regulation, Developmental
MH  - Humans
MH  - Immunologic Memory
MH  - Immunophenotyping
MH  - Leukocyte Common Antigens/genetics/isolation & purification/*metabolism
MH  - Membrane Glycoproteins/*biosynthesis/genetics/isolation & purification
MH  - NAD+ Nucleosidase/analysis
MH  - Palatine Tonsil/cytology
MH  - Phosphorylation
MH  - Protein Processing, Post-Translational
MH  - Protein Tyrosine Phosphatases/genetics/isolation & purification/*metabolism
MH  - Receptor-Like Protein Tyrosine Phosphatases, Class 3
MH  - *Semaphorins
MH  - Signal Transduction
EDAT- 2000/01/29 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/01/29 09:00
PHST- 2000/01/29 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/01/29 09:00 [entrez]
PST - ppublish
SO  - Blood. 2000 Feb 1;95(3):965-72.