PMID- 10648246
OWN - NLM
STAT- MEDLINE
DCOM- 20000317
LR  - 20220215
IS  - 0950-1991 (Print)
IS  - 0950-1991 (Linking)
VI  - 127
IP  - 4
DP  - 2000 Feb
TI  - OVO transcription factors function antagonistically in the Drosophila female 
      germline.
PG  - 881-92
AB  - OVO controls germline and epidermis differentiation in flies and mice. In the 
      Drosophila germline, alternative OVO-B and OVO-A isoforms have a common 
      DNA-binding domain, but different N-termini. We show that these isoforms are 
      transcription factors with opposite regulatory activities. Using yeast one-hybrid 
      assays, we identified a strong activation domain within a common region and a 
      counteracting repression domain within the OVO-A-specific region. In flies, OVO-B 
      positively regulated the ovarian tumor promoter, while OVO-A was a negative 
      regulator of the ovarian tumor and ovo promoters. OVO-B isoforms supplied ovo(+) 
      function in the female germline and epidermis, while OVO-A isoforms had 
      dominant-negative activity in both tissues. Moreover, elevated expression of 
      OVO-A resulted in maternal-effect lethality while the absence of OVO-A resulted 
      in maternal-effect sterility. Our data indicate that tight regulation of 
      antagonistic OVO-B and OVO-A isoforms is critical for germline formation and 
      differentiation.
FAU - Andrews, J
AU  - Andrews J
AD  - Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of 
      Health, Bethesda MD, USA.
FAU - Garcia-Estefania, D
AU  - Garcia-Estefania D
FAU - Delon, I
AU  - Delon I
FAU - Lu, J
AU  - Lu J
FAU - Mevel-Ninio, M
AU  - Mevel-Ninio M
FAU - Spierer, A
AU  - Spierer A
FAU - Payre, F
AU  - Payre F
FAU - Pauli, D
AU  - Pauli D
FAU - Oliver, B
AU  - Oliver B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Development
JT  - Development (Cambridge, England)
JID - 8701744
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (En protein, Drosophila)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Ovo1 protein, mouse)
RN  - 0 (Protein Isoforms)
RN  - 0 (Transcription Factors)
RN  - 0 (ovo protein, Drosophila)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Animals, Genetically Modified
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Drosophila/genetics/*growth & development/*metabolism
MH  - *Drosophila Proteins
MH  - Female
MH  - Genes, Reporter
MH  - Homeodomain Proteins/genetics/metabolism
MH  - Mice
MH  - Models, Biological
MH  - Molecular Sequence Data
MH  - Oogenesis
MH  - Ovary/growth & development/metabolism
MH  - Promoter Regions, Genetic
MH  - Protein Isoforms/genetics/metabolism
MH  - Saccharomyces cerevisiae/genetics
MH  - Transcription Factors/genetics/*metabolism
EDAT- 2000/01/29 09:00
MHDA- 2000/03/25 09:00
CRDT- 2000/01/29 09:00
PHST- 2000/01/29 09:00 [pubmed]
PHST- 2000/03/25 09:00 [medline]
PHST- 2000/01/29 09:00 [entrez]
AID - 10.1242/dev.127.4.881 [doi]
PST - ppublish
SO  - Development. 2000 Feb;127(4):881-92. doi: 10.1242/dev.127.4.881.