PMID- 10648228
OWN - NLM
STAT- MEDLINE
DCOM- 20000317
LR  - 20220215
IS  - 0950-1991 (Print)
IS  - 0950-1991 (Linking)
VI  - 127
IP  - 4
DP  - 2000 Feb
TI  - Generation of neurons by transient expression of neural bHLH proteins in
      mammalian cells.
PG  - 693-702
AB  - Basic helix-loop-helix (bHLH) transcription factors are known to function during 
      mammalian neurogenesis. Here we show that transient transfection of vectors
      expressing neuroD2, MASH1, ngn1 or related neural bHLH proteins, with their
      putative dimerization partner E12, can convert mouse P19 embryonal carcinoma
      cells into differentiated neurons. Transfected cells express numerous
      neuron-specific proteins, adopt a neuronal morphology and are electrically
      excitable. Thus, the expression of neural bHLH proteins is sufficient to confer a
      neuronal fate on uncommitted mammalian cells. Neuronal differentiation of
      transfected cells is preceded by elevated expression of the cyclin-dependent
      kinase inhibitor p27(Kip1) and cell cycle withdrawal. This demonstrates that the 
      bHLH proteins can link neuronal differentiation to withdrawal from the cell
      cycle, possibly by activating the expression of p27(Kip1). The ability to
      generate mammalian neurons by transient expression of neural bHLH proteins should
      create new opportunities for studying neurogenesis and devising neural repair
      strategies.
FAU - Farah, M H
AU  - Farah MH
AD  - Mental Health Research Institute, Neuroscience Program, and Department of
      Biology, University of Michigan, Ann Arbor, MI 48104-1687, USA.
FAU - Olson, J M
AU  - Olson JM
FAU - Sucic, H B
AU  - Sucic HB
FAU - Hume, R I
AU  - Hume RI
FAU - Tapscott, S J
AU  - Tapscott SJ
FAU - Turner, D L
AU  - Turner DL
LA  - eng
GR  - P30 HD28834/HD/NICHD NIH HHS/United States
GR  - R01NS36086/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Development
JT  - Development (Cambridge, England)
JID - 8701744
RN  - 0 (Ascl1 protein, mouse)
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (Cdkn1b protein, mouse)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (NEUROD2 protein, human)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurod2 protein, mouse)
RN  - 0 (Neuropeptides)
RN  - 0 (Transcription Factors)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 147604-94-2 (Cyclin-Dependent Kinase Inhibitor p27)
RN  - 182238-50-2 (Neurog1 protein, mouse)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinases)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Basic Helix-Loop-Helix Transcription Factors
MH  - Cell Cycle
MH  - *Cell Cycle Proteins
MH  - Cell Differentiation
MH  - Cell Line
MH  - Cyclin-Dependent Kinase Inhibitor p27
MH  - Cyclin-Dependent Kinases/antagonists & inhibitors
MH  - DNA Primers/genetics
MH  - DNA-Binding Proteins/genetics
MH  - Gene Expression Regulation, Developmental
MH  - Genetic Vectors
MH  - Helix-Loop-Helix Motifs/*genetics
MH  - Mice
MH  - Microtubule-Associated Proteins/genetics
MH  - Nerve Tissue Proteins/genetics
MH  - Neurons/*cytology/*metabolism
MH  - Neuropeptides/genetics
MH  - Transcription Factors/*genetics
MH  - Transfection
MH  - *Tumor Suppressor Proteins
EDAT- 2000/01/29 09:00
MHDA- 2000/03/25 09:00
CRDT- 2000/01/29 09:00
PHST- 2000/01/29 09:00 [pubmed]
PHST- 2000/03/25 09:00 [medline]
PHST- 2000/01/29 09:00 [entrez]
AID - 10.1242/dev.127.4.693 [doi]
PST - ppublish
SO  - Development. 2000 Feb;127(4):693-702. doi: 10.1242/dev.127.4.693.