PMID- 10647823
OWN - NLM
STAT- MEDLINE
DCOM- 20000327
LR  - 20211203
IS  - 1042-5179 (Print)
IS  - 1026-7913 (Linking)
VI  - 10
IP  - 3
DP  - 1999
TI  - Cloning and characterization of the human novel gene encoding 6.2 kDa protein
      highly expressed upon ultraviolet irradiation in DNA-PK-deleted human glioma
      M059J cells.
PG  - 195-8
AB  - The full length of a gene isolated using a methylated DNA binding column as
      previously reported (Cross et al., 1994) has been cloned. The gene encodes 55
      amino acids (6.2 kDa), and has a transcript of approximately 0.45 kb. It has 2
      exons in the genome. After searching for sequence homology (BLAST), a homology to
      hypothetically 6.3 kDa protein located at cosmid ZK652 (protein P34660; EMBL
      Accession No. L14429) of C. elegans chromosome 3 was noted (51.9% amino acid
      homology). It has 1 site (-SQ-) for DNA-dependent protein kinase (DNA-PK)
      phosphorylation and 2 sites of myristilation. This novel gene is very strongly
      expressed (about 25.6 times) in DNA-PK-deleted human glioma cell line (M059J) 2
      hrs after ultraviolet (UV) irradiation compared to the DNA-PK +/+ cell line
      (M059K) which expresses the gene at a relatively low level (about 7.5 times).
      Therefore, this result suggests that DNA-PK may negatively regulate the
      transcriptional level of this gene in M059K cells upon UV damage (50 J/m2),
      associated with cell toxicity.
FAU - Lee, J S
AU  - Lee JS
AD  - Institute for Molecular Biology and Genetics, Seoul National University, South
      Korea. jslee2@plazal.snu.ac.kr
LA  - eng
SI  - GENBANK/AJ011007
PT  - Journal Article
PL  - England
TA  - DNA Seq
JT  - DNA sequence : the journal of DNA sequencing and mapping
JID - 9107800
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Helminth Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Mitochondrial Precursor Protein Import Complex Proteins)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Proteins)
RN  - 0 (TOMM7 protein, human)
RN  - EC 2.7.11.1 (DNA-Activated Protein Kinase)
RN  - EC 2.7.11.1 (PRKDC protein, human)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cell Survival/radiation effects
MH  - Central Nervous System Neoplasms/*genetics/*radiotherapy
MH  - Cloning, Molecular
MH  - DNA Damage/radiation effects
MH  - DNA Methylation
MH  - DNA-Activated Protein Kinase
MH  - *DNA-Binding Proteins
MH  - Dose-Response Relationship, Radiation
MH  - Gene Expression Regulation, Neoplastic/radiation effects
MH  - Glioma/enzymology/*genetics/*radiotherapy
MH  - Helminth Proteins/genetics/metabolism
MH  - Humans
MH  - Membrane Proteins
MH  - Mitochondrial Precursor Protein Import Complex Proteins
MH  - Mitochondrial Proteins
MH  - Molecular Sequence Data
MH  - Nuclear Proteins
MH  - Phosphorylation
MH  - Protein Serine-Threonine Kinases/genetics/metabolism/radiation effects
MH  - Proteins/*genetics/*metabolism
MH  - Sequence Homology, Amino Acid
MH  - Tumor Cells, Cultured
MH  - Ultraviolet Rays
EDAT- 2000/01/27 09:00
MHDA- 2000/04/01 09:00
CRDT- 2000/01/27 09:00
PHST- 2000/01/27 09:00 [pubmed]
PHST- 2000/04/01 09:00 [medline]
PHST- 2000/01/27 09:00 [entrez]
AID - 10.3109/10425179909033947 [doi]
PST - ppublish
SO  - DNA Seq. 1999;10(3):195-8. doi: 10.3109/10425179909033947.