PMID- 10646860
OWN - NLM
STAT- MEDLINE
DCOM- 20000204
LR  - 20220330
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 60
IP  - 1
DP  - 2000 Jan 1
TI  - APCL, a central nervous system-specific homologue of adenomatous polyposis coli
      tumor suppressor, binds to p53-binding protein 2 and translocates it to the
      perinucleus.
PG  - 101-5
AB  - APCL, a central nervous system-specific sequence homologue of the adenomatous
      polyposis coli tumor suppressor, can regulate the cytoplasmic level of
      beta-catenin as the adenomatous polyposis coli tumor suppressor does, but its
      overall biological function remains unclear. Using a yeast two-hybrid system, we 
      attempted to isolate proteins that might associate with the unique COOH-terminus 
      of APCL. Among 166 cDNA clones isolated from a human fetal-brain cDNA library as 
      candidates for interaction with APCL, 32 encoded parts of p53-binding protein 2
      (53BP2), a molecule that interacts with p53 and Bcl2. An in vitro binding assay
      indicated that the Src-homology-3 domain and the ankyrin-repeat domain of 53BP2
      were both required for binding to the COOH-terminus of APCL. Confocal microscopy 
      showed that APCL and 53BP2 proteins were localized together in the perinuclei of 
      normal mammalian cells, but this was not the case in cells that expressed
      truncated APCL and 53BP2 proteins. These findings suggested that binding of the
      COOH-terminus of APCL to 53BP2 regulates the cytoplasmic location of 53BP2.
      Because 53BP2 also interacts with p53 and Bcl2 and regulates p53 function, our
      results suggest that APCL might be involved in the p53/Bcl2-linked pathway of
      cell-cycle progression and cell death.
FAU - Nakagawa, H
AU  - Nakagawa H
AD  - Biomedical Research Center and the Second Department of Surgery, Osaka University
      Medical School, Japan.
FAU - Koyama, K
AU  - Koyama K
FAU - Murata, Y
AU  - Murata Y
FAU - Morito, M
AU  - Morito M
FAU - Akiyama, T
AU  - Akiyama T
FAU - Nakamura, Y
AU  - Nakamura Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (APC2 protein, human)
RN  - 0 (Adenomatous Polyposis Coli Protein)
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (TP53BP2 protein, human)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Adenomatous Polyposis Coli Protein
MH  - Animals
MH  - Apoptosis Regulatory Proteins
MH  - Blotting, Western
MH  - COS Cells
MH  - Carrier Proteins/genetics/*metabolism
MH  - Cell Nucleus/metabolism
MH  - Cytoskeletal Proteins/genetics/*metabolism
MH  - Genetic Vectors/genetics/metabolism
MH  - Glutathione Transferase/genetics/metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Microscopy, Confocal
MH  - Plasmids/genetics
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Transfection
MH  - beta-Galactosidase/genetics/metabolism
EDAT- 2000/01/26 00:00
MHDA- 2000/01/26 00:01
CRDT- 2000/01/26 00:00
PHST- 2000/01/26 00:00 [pubmed]
PHST- 2000/01/26 00:01 [medline]
PHST- 2000/01/26 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 2000 Jan 1;60(1):101-5.