PMID- 10646847
OWN - NLM
STAT- MEDLINE
DCOM- 20000204
LR  - 20171116
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 60
IP  - 1
DP  - 2000 Jan 1
TI  - Threonine phosphorylation of the MMAC1/PTEN PDZ binding domain both inhibits and 
      stimulates PDZ binding.
PG  - 35-7
AB  - Two-hybrid searches with the tumor suppressor MMAC1/PTEN isolated the proteins
      hDLG and hMAST205. Further two-hybrid analysis and microtiter plate binding
      assays localized the sites of interaction to PDZ domains from hDLG and hMAST205
      and the PDZ binding domain at the COOH terminus of MMAC1/PTEN. A synthetic
      peptide derived from the MMAC1/PTEN PDZ binding domain (MMAC1/PTEN-PDZBD) was
      used to coprecipitate proteins from A431 human cell lysate. The recovered
      proteins were resolved by SDS-PAGE and immobilized on a nitrocellulose membrane. 
      Treatment of this membrane with an anti-hDLG antibody identified a Mr 140,000
      band, consistent with the size of hDLG. Treatment of this membrane with the
      MMAC1/PTEN-PDZBD peptide identified a single prominent band of slightly larger
      than Mr 200,000 (Mr 200,000 kDa). Threonine phosphorylation of the MMAC1/
      PTEN-PDZBD peptide inhibited both microtiter plate binding to the hDLG and
      hMAST205 PDZ domains and coprecipitation of the Mr 140,000 and > 200,000
      proteins, but promoted coprecipitation of proteins of approximately Mr 90,000 and
      Mr 120,000 from A431 cell lysate. This result suggests phosphorylation of the
      MMAC1/PTEN PDZ binding domain can both inhibit and promote PDZ interactions.
FAU - Adey, N B
AU  - Adey NB
AD  - Myriad Genetics Inc., Salt Lake City, Utah 84108, USA. nils@myriad.com
FAU - Huang, L
AU  - Huang L
FAU - Ormonde, P A
AU  - Ormonde PA
FAU - Baumgard, M L
AU  - Baumgard ML
FAU - Pero, R
AU  - Pero R
FAU - Byreddy, D V
AU  - Byreddy DV
FAU - Tavtigian, S V
AU  - Tavtigian SV
FAU - Bartel, P L
AU  - Bartel PL
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (DLG1 protein, human)
RN  - 0 (Discs Large Homolog 1 Protein)
RN  - 0 (Dlg1 protein, mouse)
RN  - 0 (Membrane Proteins)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 2ZD004190S (Threonine)
RN  - EC 2.7.4.8 (Guanylate Kinases)
RN  - EC 3.1.3.2 (Phosphoric Monoester Hydrolases)
RN  - EC 3.1.3.67 (PTEN Phosphohydrolase)
RN  - EC 3.1.3.67 (PTEN protein, human)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Animals
MH  - Carrier Proteins/genetics/*physiology
MH  - Discs Large Homolog 1 Protein
MH  - Guanylate Kinases
MH  - Humans
MH  - Membrane Proteins
MH  - Mice
MH  - PTEN Phosphohydrolase
MH  - Phosphoric Monoester Hydrolases/genetics/*metabolism
MH  - Phosphorylation
MH  - Proteins/*metabolism
MH  - Recombinant Fusion Proteins/genetics/physiology
MH  - Threonine/*metabolism
MH  - Tumor Cells, Cultured
MH  - *Tumor Suppressor Proteins
EDAT- 2000/01/26 00:00
MHDA- 2000/01/26 00:01
CRDT- 2000/01/26 00:00
PHST- 2000/01/26 00:00 [pubmed]
PHST- 2000/01/26 00:01 [medline]
PHST- 2000/01/26 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 2000 Jan 1;60(1):35-7.