PMID- 10646504
OWN - NLM
STAT- MEDLINE
DCOM- 20000210
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 74
IP  - 2
DP  - 2000 Feb
TI  - Selective synthesis of 2',3'-cyclic nucleotide 3'-phosphodiesterase isoform 2 and
      identification of specifically phosphorylated serine residues.
PG  - 540-6
AB  - 2',3'-Cyclic nucleotide 3'-phosphodiesterase (CNP) is a protein found abundantly 
      in the cytoplasmic compartments of CNS myelin. Two isoforms of this protein, CNP1
      and CNP2, are detectable. They differ by a 20-amino acid extension exclusive to
      CNP2. Additionally, CNP2 is essentially the only isoform to be phosphorylated in 
      vivo. In this study, we examine the phosphorylation of CNP2 in transfected cells.
      CNP2 was selectively expressed ectopically in 293T cells and labeled with 32P.
      Immunoprecipitation of labeled CNP2 and tryptic phosphopeptide mapping analyses
      identified serines 9 and 22 as the major sites of phosphorylation. Only serine 22
      was phosphorylated initially in oligodendrocyte-enriched cultures of neonatal rat
      brain glial cells. However, 4beta-phorbol 12,13-dibutyrate (PDB) induced the
      phosphorylation of serine 9, thereby producing the same pattern seen in 293T
      cells. These results suggest that serine 9 is phosphorylated by a PDB-sensitive
      kinase, likely protein kinase C, and that serine 22 appears to be constitutively 
      phosphorylated.
FAU - O'Neill, R C
AU  - O'Neill RC
AD  - Department of Biochemistry, McGill University, Montreal, Quebec, Canada.
FAU - Braun, P E
AU  - Braun PE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Isoenzymes)
RN  - 0 (Phosphopeptides)
RN  - 37558-16-0 (Phorbol 12,13-Dibutyrate)
RN  - 452VLY9402 (Serine)
RN  - EC 3.1.4.- (2',3'-Cyclic-Nucleotide Phosphodiesterases)
RN  - EC 3.1.4.- (Phosphoric Diester Hydrolases)
RN  - EC 3.1.4.37 (2',3'-Cyclic Nucleotide 3'-Phosphodiesterase)
RN  - EC 3.1.4.37 (CNP protein, human)
RN  - EC 3.1.4.37 (Cnp protein, rat)
RN  - EC 3.4.21.4 (Trypsin)
SB  - IM
MH  - 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase
MH  - 2',3'-Cyclic-Nucleotide Phosphodiesterases/*biosynthesis/*chemistry/genetics
MH  - Amino Acid Sequence/genetics
MH  - Animals
MH  - Humans
MH  - Isoenzymes/*biosynthesis/*chemistry/genetics
MH  - Oligodendroglia/metabolism
MH  - *Peptide Mapping
MH  - Phorbol 12,13-Dibutyrate/pharmacology
MH  - Phosphopeptides/genetics/metabolism
MH  - *Phosphoric Diester Hydrolases
MH  - Phosphorylation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Serine/*chemistry/*metabolism
MH  - Stem Cells/metabolism
MH  - Trypsin/metabolism
EDAT- 2000/01/26 00:00
MHDA- 2000/01/26 00:01
CRDT- 2000/01/26 00:00
PHST- 2000/01/26 00:00 [pubmed]
PHST- 2000/01/26 00:01 [medline]
PHST- 2000/01/26 00:00 [entrez]
AID - 10.1046/j.1471-4159.2000.740540.x [doi]
PST - ppublish
SO  - J Neurochem. 2000 Feb;74(2):540-6. doi: 10.1046/j.1471-4159.2000.740540.x.