PMID- 10644855
OWN - NLM
STAT- MEDLINE
DCOM- 20000229
LR  - 20201208
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 81
IP  - Pt 2
DP  - 2000 Feb
TI  - Molecular characterization of the genome of a partitivirus from the basidiomycete
      Rhizoctonia solani.
PG  - 549-55
AB  - The bisegmented genome of a double-stranded (ds) RNA virus from the fungus
      Rhizoctonia solani isolate Rhs 717 was characterized. The larger segment, dsRNA
      1, is 2363 bases long whereas the smaller segment, dsRNA 2, has 2206 bases. The
      5' ends of the coding strands of dsRNA 1 and dsRNA 2 are highly conserved (100%
      identity over 47 bases), and contain inverted repeats capable of forming stable
      stem-loop structures. Analysis of the coding potential of each of the two
      segments showed that dsRNAs 1 and 2 could code for polypeptides of 730 aa (bases 
      86-2275; molecular mass 86 kDa) and 683 aa (bases 79-2130; molecular mass 76
      kDa), respectively. The 86 kDa polypeptide has all the motifs of dsRNA
      RNA-dependent RNA polymerases (RDRP), and has significant homology with putative 
      RDRPs of partitiviruses from Fusarium poae and Atkinsonella hypoxylon. The 76 kDa
      protein shows homology with the putative capsid proteins (CP) of the same
      viruses. Northern blot analysis revealed no subgenomic RNA species, consistent
      with the fact that the long open reading frames encoding the putative RDRP and CP
      cover the entire length of the respective dsRNAs.
FAU - Strauss, E E
AU  - Strauss EE
AD  - Department of Biological Sciences, University of Maine, Orono, ME 04469-0102,
      USA.
FAU - Lakshman, D K
AU  - Lakshman DK
FAU - Tavantzis, S M
AU  - Tavantzis SM
LA  - eng
SI  - GENBANK/AF133290
SI  - GENBANK/AF133291
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (DNA Primers)
RN  - 0 (Viral Proteins)
RN  - EC 2.7.7.48 (RNA-Dependent RNA Polymerase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Basidiomycota/*virology
MH  - Capsid/genetics
MH  - DNA Primers/genetics
MH  - Gene Expression
MH  - *Genome, Viral
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Phylogeny
MH  - RNA Viruses/classification/*genetics/*isolation & purification
MH  - RNA-Dependent RNA Polymerase/genetics
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
MH  - Viral Proteins/chemistry/genetics
EDAT- 2000/01/25 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/01/25 09:00
PHST- 2000/01/25 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/01/25 09:00 [entrez]
AID - 10.1099/0022-1317-81-2-549 [doi]
PST - ppublish
SO  - J Gen Virol. 2000 Feb;81(Pt 2):549-55. doi: 10.1099/0022-1317-81-2-549.