PMID- 10644812
OWN - NLM
STAT- MEDLINE
DCOM- 20000210
LR  - 20191210
IS  - 0041-1132 (Print)
IS  - 0041-1132 (Linking)
VI  - 40
IP  - 1
DP  - 2000 Jan
TI  - Genomic typing of human red cell miltenberger glycophorins in a Taiwanese
      population.
PG  - 54-61
AB  - BACKGROUND: Antigens in the human red cell Miltenberger series are glycophorin
      variants of the MN (MNS) blood group system that are due to the rearrangement of 
      glycophorin A (GPA) and glycophorin B (GPB) genes. STUDY DESIGN AND METHODS:
      Taking advantage of the differences between the GPA and GPB genes, a polymerase
      chain reaction-based method was developed to detect all the Miltenberger
      glycophorin variants and St(a) subtype. GPA- and GPB-specific primers were used
      to amplify the GPA or GPB gene, and the amplified products were used to recognize
      the different hybrid genes after restriction enzyme digestions. RESULTS: Among
      264 Taiwanese subjects studied, Mi.III and St(a) are the most common types of
      Miltenberger variants found. Mi.III was present in 13 (4.92%) of 264, and St(a)
      was found in 8 (3. 03%) of 264; 1 case (0.4%) of Mi.V was also identified from
      the study group. CONCLUSION: This is the first polymerase chain reaction-based
      method of detecting most of the Miltenberger variants and St(a). The genomic
      typing results were confirmed by control DNA of identified Miltenberger
      phenotypes. The prevalence rates of Mi. III and St(a) in this study were also
      consistent with other previous reports using different methods.
FAU - Shih, M C
AU  - Shih MC
AD  - Department of Laboratory Medicine, School of Pharmacy, Taichung, Taiwan
FAU - Yang, L H
AU  - Yang LH
FAU - Wang, N M
AU  - Wang NM
FAU - Chang, J G
AU  - Chang JG
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transfusion
JT  - Transfusion
JID - 0417360
RN  - 0 (Glycophorins)
RN  - 0 (MNSs Blood-Group System)
SB  - IM
MH  - Erythrocytes/chemistry
MH  - Genetic Variation
MH  - Genome
MH  - Glycophorins/*genetics
MH  - Humans
MH  - MNSs Blood-Group System/*genetics
MH  - Taiwan
EDAT- 2000/01/25 00:00
MHDA- 2000/01/25 00:01
CRDT- 2000/01/25 00:00
PHST- 2000/01/25 00:00 [pubmed]
PHST- 2000/01/25 00:01 [medline]
PHST- 2000/01/25 00:00 [entrez]
AID - 10.1046/j.1537-2995.2000.40010054.x [doi]
PST - ppublish
SO  - Transfusion. 2000 Jan;40(1):54-61. doi: 10.1046/j.1537-2995.2000.40010054.x.