PMID- 10644767
OWN - NLM
STAT- MEDLINE
DCOM- 20000229
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 4
DP  - 2000 Jan 28
TI  - Three isoforms of synaptic scaffolding molecule and their characterization.
      Multimerization between the isoforms and their interaction with
      N-methyl-D-aspartate receptors and SAP90/PSD-95-associated protein.
PG  - 2966-72
AB  - The synaptic scaffolding molecule (S-SCAM) has been identified as a protein
      interacting with SAP90/PSD-95-associated protein (SAPAP) (also called guanylate
      kinase-associated protein/hDLG-associated protein). S-SCAM has six PDZ (we have
      numbered them PDZ-0 to -5), two WW, and one guanylate kinase (GK) domains and
      interacts with N-methyl-D-aspartate (NMDA) receptor via PDZ-5 and SAPAP via the
      GK domain. We have identified here shorter isoforms of S-SCAM that start at the
      164th or 224th methionine, and we renamed the original one, S-SCAMalpha, the
      middle one, S-SCAMbeta, and the shortest one, S-SCAM-gamma. S-SCAMbeta and -gamma
      have five PDZ (PDZ-1 to -5), two WW, and one GK domains. S-SCAMalpha interacted
      with S-SCAMbeta and -gamma through the region containing PDZ-4 and -5. The region
      containing both of PDZ-4 and -5 is sufficient for the clustering of NMDA
      receptors and forms a dimer in gel filtration, suggesting that S-SCAM forms
      multimers via the interaction between the C-terminal PDZ domains and assembles
      NMDA receptors into clusters. S-SCAMbeta and -gamma also interacted with SAPAP,
      suggesting that the N-terminal region of the GK domain is not necessary for the
      interaction. Finally, we have identified the interaction of the PDZ domains of
      S-SCAM with the GK domain of PSD-95/SAP90. S-SCAM, PSD-95/SAP90, and SAPAP are
      colocalized at least in some part in brain. Therefore, S-SCAM, PSD-95/SAP90, and 
      SAPAP may form a complex in vivo.
FAU - Hirao, K
AU  - Hirao K
AD  - Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, JCR
      Pharmaceuticals Company Limited, 2-2-10 Murotani, Nishi-ku, Kobe 651-2241, Japan.
FAU - Hata, Y
AU  - Hata Y
FAU - Yao, I
AU  - Yao I
FAU - Deguchi, M
AU  - Deguchi M
FAU - Kawabe, H
AU  - Kawabe H
FAU - Mizoguchi, A
AU  - Mizoguchi A
FAU - Takai, Y
AU  - Takai Y
LA  - eng
SI  - GENBANK/AF130819
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Biopolymers)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (Magi2 protein, rat)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (SAP90-PSD95 Associated Proteins)
RN  - EC 2.7.4.8 (Guanylate Kinases)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Biopolymers
MH  - CHO Cells
MH  - COS Cells
MH  - Carrier Proteins/chemistry/*metabolism
MH  - Cerebellum/metabolism
MH  - Chromatography, Gel
MH  - Cricetinae
MH  - DNA Primers
MH  - Guanylate Kinases
MH  - Microscopy, Fluorescence
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/chemistry/*metabolism
MH  - Protein Binding
MH  - Protein Isoforms/chemistry/*metabolism
MH  - Rats
MH  - Receptors, N-Methyl-D-Aspartate/*metabolism
MH  - Retina/metabolism
MH  - SAP90-PSD95 Associated Proteins
MH  - Sequence Homology, Amino Acid
EDAT- 2000/01/25 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/01/25 09:00
PHST- 2000/01/25 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/01/25 09:00 [entrez]
AID - 10.1074/jbc.275.4.2966 [doi]
AID - S0021-9258(18)31062-7 [pii]
PST - ppublish
SO  - J Biol Chem. 2000 Jan 28;275(4):2966-72. doi: 10.1074/jbc.275.4.2966.