PMID- 10644752
OWN - NLM
STAT- MEDLINE
DCOM- 20000229
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 4
DP  - 2000 Jan 28
TI  - Cloning of factors related to HIV-inducible LBP proteins that regulate
      steroidogenic factor-1-independent human placental transcription of the
      cholesterol side-chain cleavage enzyme, P450scc.
PG  - 2852-8
AB  - The cholesterol side-chain cleavage enzyme, cytochrome P450scc, initiates the
      biosynthesis of all steroid hormones. Adrenal and gonadal strategies for P450scc 
      gene transcription are essentially identical and depend on the orphan nuclear
      receptor steroidogenic factor-1, but the placental strategy for transcription of 
      P450scc employs cis-acting elements different from those used in the adrenal
      strategy and is independent of steroidogenic factor-1. Because placental
      expression of P450scc is required for human pregnancy, we sought factors that
      bind to the -155/-131 region of the human P450scc promoter, which participates in
      its placental but not adrenal or gonadal transcription. A yeast one-hybrid screen
      of 2.4 x 10(6) cDNA clones from human placental JEG-3 cells yielded two unique
      clones; one is the previously described transcription factor LBP-1b, which is
      induced by HIV, type I infection of lymphocytes, and the other is a new factor,
      termed LBP-9, that shares 83% amino acid sequence identity with LBP-1b. When
      expressed in transfected yeast, both factors bound specifically to the -155/-131 
      DNA; antisera to LBP proteins supershifted the LBP-9.DNA complex and inhibited
      formation of the LBP-1b.DNA complex. Reverse transcriptase-polymerase chain
      reaction detected LBP-1b in human placental JEG-3, adrenal NCI-H295A, liver
      HepG2, cervical HeLa, and monkey kidney COS-1 cells, but LBP-9 was detected only 
      in JEG-3 cells. When the -155/-131 fragment was linked to a minimal promoter,
      co-expression of LBP-1b increased transcription 21-fold in a dose-dependent
      fashion, but addition of LBP-9 suppressed the stimulatory effect of LBP-1b. The
      roles of LBP transcription factors in normal human physiology have been unclear. 
      Their modulation of placental but not adrenal P450scc transcription underscores
      the distinctiveness of placental strategies for steroidogenic enzyme gene
      transcription.
FAU - Huang, N
AU  - Huang N
AD  - Department of Pediatrics, University of California, San Francisco, San Francisco,
      California 94143-0978, USA.
FAU - Miller, W L
AU  - Miller WL
LA  - eng
GR  - DK 37922/DK/NIDDK NIH HHS/United States
GR  - DK 42154/DK/NIDDK NIH HHS/United States
GR  - HD 34449/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Fushi Tarazu Transcription Factors)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (NR5A1 protein, human)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Repressor Proteins)
RN  - 0 (Steroidogenic Factor 1)
RN  - 0 (TFCP2L1 protein, human)
RN  - 0 (Tcfcp2l1 protein, mouse)
RN  - 0 (Transcription Factors)
RN  - 0 (steroidogenic factor 1, mouse)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 1.14.15.6 (Cholesterol Side-Chain Cleavage Enzyme)
SB  - IM
SB  - X
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cholesterol/metabolism
MH  - Cholesterol Side-Chain Cleavage Enzyme/*genetics/metabolism
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - DNA-Binding Proteins/*genetics/*metabolism
MH  - Fushi Tarazu Transcription Factors
MH  - *Gene Expression Regulation, Enzymologic
MH  - HIV-1/*physiology
MH  - Homeodomain Proteins
MH  - Humans
MH  - Hydrolysis
MH  - Molecular Sequence Data
MH  - Placenta/*metabolism
MH  - Promoter Regions, Genetic
MH  - Protein Binding
MH  - RNA-Binding Proteins
MH  - Receptors, Cytoplasmic and Nuclear
MH  - *Repressor Proteins
MH  - Sequence Homology, Amino Acid
MH  - Steroidogenic Factor 1
MH  - Transcription Factors/*genetics/*metabolism
MH  - *Transcription, Genetic
EDAT- 2000/01/25 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/01/25 09:00
PHST- 2000/01/25 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/01/25 09:00 [entrez]
AID - 10.1074/jbc.275.4.2852 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Jan 28;275(4):2852-8. doi: 10.1074/jbc.275.4.2852.