PMID- 10644739
OWN - NLM
STAT- MEDLINE
DCOM- 20000229
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 4
DP  - 2000 Jan 28
TI  - Molecular cloning of an N-terminal splice variant of the capsaicin receptor. Loss
      of N-terminal domain suggests functional divergence among capsaicin receptor
      subtypes.
PG  - 2756-62
AB  - Recently a cDNA clone, vanilloid receptor subtype-1 (VR1), was isolated and found
      to encode an ion channel that is activated by both capsaicin, the pain producing 
      compound in chili peppers, and by noxious thermal stimuli. Subsequently, two
      related cDNAs have been isolated, a stretch inactivating channel with
      mechanosensitive properties and a vanilloid receptor-like protein that is
      responsive to high temperatures (52-53 degrees C). Here, we report the isolation 
      of a vanilloid receptor 5'-splice variant (VR.5'sv) which differs from VR1 by
      elimination of the majority of the intracellular N-terminal domain and ankyrin
      repeat elements. Both VR.5'sv and VR1 mRNA were shown to be expressed in tissues 
      reportedly responsive to capsaicin including dorsal root ganglion, brain, and
      peripheral blood mononuclear cells. Functional expression of VR.5'sv in Xenopus
      oocytes and mammalian cells showed no sensitivity to capsaicin, the potent
      vanilloid resiniferatoxin, hydrogen ions (pH 6.2), or noxious thermal stimuli (50
      degrees C). Since VR.5'sv is otherwise identical to VR1 throughout its
      transmembrane spanning domains and C-terminal region, these results support the
      hypothesis that the N-terminal intracellular domain is essential for the
      formation of functional receptors activated by vanilloid compounds and noxious
      thermal stimuli.
FAU - Schumacher, M A
AU  - Schumacher MA
AD  - Department of Anesthesia, University of California, San Francisco, California
      94143, USA. schumac@itsa.ucsf.edu
FAU - Moff, I
AU  - Moff I
FAU - Sudanagunta, S P
AU  - Sudanagunta SP
FAU - Levine, J D
AU  - Levine JD
LA  - eng
SI  - GENBANK/AF158248
GR  - NS21647/NS/NINDS NIH HHS/United States
GR  - NS38737-01/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Drug)
SB  - IM
SB  - S
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Ganglia, Spinal/metabolism
MH  - Molecular Sequence Data
MH  - *RNA Splicing
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Receptors, Drug/chemistry/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Nucleic Acid
MH  - Xenopus
EDAT- 2000/01/25 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/01/25 09:00
PHST- 2000/01/25 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/01/25 09:00 [entrez]
AID - 10.1074/jbc.275.4.2756 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Jan 28;275(4):2756-62. doi: 10.1074/jbc.275.4.2756.