PMID- 10644725
OWN - NLM
STAT- MEDLINE
DCOM- 20000229
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 4
DP  - 2000 Jan 28
TI  - Molecular cloning and characterization of Amida, a novel protein which interacts 
      with a neuron-specific immediate early gene product arc, contains novel nuclear
      localization signals, and causes cell death in cultured cells.
PG  - 2647-53
AB  - Amida was isolated by the yeast two-hybrid system as a novel protein which
      associated with Arc, a non-transcriptional immediate early gene specific to the
      brain. Amida was confirmed to be associated with Arc in vitro and in vivo. Amida 
      shows no homology to known proteins. Amida is ubiquitously expressed, although it
      is abundant in the brain. A transfection study revealed that Amida was localized 
      in the nucleus and after 72 h the transfected cells underwent apoptosis.
      Furthermore, we found two nuclear localization signals and a domain needed for
      interacting with Arc was encompassed by two nuclear localization signals.
      Co-transfection experiment with Amida and Arc suggested that Amida transported
      Arc into the nucleus and negatively regulated Amida-induced cell death. These
      results indicate that Arc together with Amida may modulate cell death in the
      brain.
FAU - Irie, Y
AU  - Irie Y
AD  - Department of Pharmacology, Osaka University Medical School, 2-2 Yamadaoka,
      Suita, Osaka 565-0871, Japan.
FAU - Yamagata, K
AU  - Yamagata K
FAU - Gan, Y
AU  - Gan Y
FAU - Miyamoto, K
AU  - Miyamoto K
FAU - Do, E
AU  - Do E
FAU - Kuo, C H
AU  - Kuo CH
FAU - Taira, E
AU  - Taira E
FAU - Miki, N
AU  - Miki N
LA  - eng
SI  - GENBANK/UNKNOWN
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nuclear Localization Signals)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Tfpt protein, rat)
RN  - 0 (activity regulated cytoskeletal-associated protein)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Apoptosis/*genetics
MH  - Base Sequence
MH  - COS Cells
MH  - Cell Nucleus/metabolism
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - Cytoskeletal Proteins/*metabolism
MH  - DNA, Complementary
MH  - *Genes, Immediate-Early
MH  - Male
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/*metabolism
MH  - Neurons/*metabolism
MH  - *Nuclear Localization Signals
MH  - Nuclear Proteins/*genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Subcellular Fractions/metabolism
MH  - Two-Hybrid System Techniques
EDAT- 2000/01/25 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/01/25 09:00
PHST- 2000/01/25 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/01/25 09:00 [entrez]
AID - 10.1074/jbc.275.4.2647 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Jan 28;275(4):2647-53. doi: 10.1074/jbc.275.4.2647.