PMID- 10644691
OWN - NLM
STAT- MEDLINE
DCOM- 20000229
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 4
DP  - 2000 Jan 28
TI  - Protein phosphatase 2Calpha dephosphorylates axin and activates LEF-1-dependent
      transcription.
PG  - 2399-403
AB  - The Dishevelled (Dvl) gene family encodes cytoplasmic proteins that are necessary
      for Wnt signal transduction. Utilizing the yeast two-hybrid system, we identified
      protein phosphatase 2Calpha (PP2C) as a Dvl-PDZ domain-interacting protein. PP2C 
      exists in a complex with Dvl, beta-catenin, and Axin, a negative regulator of Wnt
      signaling. In a Wnt-responsive LEF-1 reporter gene assay, expression of PP2C
      activates transcription and also elicits a synergistic response with beta-catenin
      and Wnt-1. In addition, PP2C expression relieves Axin-mediated repression of
      LEF-1-dependent transcription. PP2C utilizes Axin as a substrate both in vitro
      and in vivo and decreases its half-life. These results indicate that PP2C is a
      positive regulator of Wnt signal transduction and mediates its effects through
      the dephosphorylation of Axin.
FAU - Strovel, E T
AU  - Strovel ET
AD  - Division of Human Genetics, University of Maryland School of Medicine, Baltimore,
      Maryland 21201, USA.
FAU - Wu, D
AU  - Wu D
FAU - Sussman, D J
AU  - Sussman DJ
LA  - eng
GR  - R01 CA63929/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Axin Protein)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Dishevelled Proteins)
RN  - 0 (Lymphoid Enhancer-Binding Factor 1)
RN  - 0 (Phosphoproteins)
RN  - 0 (Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.1.3.16 (PTC1 protein, S cerevisiae)
RN  - EC 3.1.3.16 (Phosphoprotein Phosphatases)
RN  - EC 3.1.3.16 (Protein Phosphatase 2)
RN  - EC 3.1.3.16 (Protein Phosphatase 2C)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Animals
MH  - Axin Protein
MH  - COS Cells
MH  - DNA-Binding Proteins/*metabolism
MH  - Dishevelled Proteins
MH  - Enzyme Activation
MH  - JNK Mitogen-Activated Protein Kinases
MH  - Lymphoid Enhancer-Binding Factor 1
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Phosphoprotein Phosphatases/*metabolism
MH  - Phosphoproteins/metabolism
MH  - Phosphorylation
MH  - Precipitin Tests
MH  - Protein Binding
MH  - Protein Phosphatase 2
MH  - Protein Phosphatase 2C
MH  - Proteins/*metabolism
MH  - *Repressor Proteins
MH  - *Saccharomyces cerevisiae Proteins
MH  - Signal Transduction
MH  - Transcription Factors/*metabolism
MH  - *Transcriptional Activation
EDAT- 2000/01/25 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/01/25 09:00
PHST- 2000/01/25 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/01/25 09:00 [entrez]
AID - 10.1074/jbc.275.4.2399 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Jan 28;275(4):2399-403. doi: 10.1074/jbc.275.4.2399.